二烯丙基二硫化物通过减轻氧化应激、细胞凋亡、TLR-4/NF-κB和JAK1/STAT3信号传导以及上调SIRT1，预防镉对大鼠睾丸的损伤。

IF 3.6 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Trace Elements in Medicine and Biology Pub Date : 2024-11-12 DOI:10.1016/j.jtemb.2024.127560

Emad H.M. Hassanein , Mohammed F. Alotaibi , Reem S. Alruhaimi , Omnia A.M. Abd El-Ghafar , Mostafa K. Mohammad , Ahmed M. Atwa , Ayman M. Mahmoud

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引用次数: 0

摘要

背景：镉（Cd）是一种可导致严重健康问题的重金属环境污染物。镉可导致睾丸结构改变，接触这种重金属与精子丧失和男性不育有关。人们已经认识到氧化应激和炎症在镉毒性中的作用。二烯丙基二硫化物（DADS）是大蒜中的一种有机硫化合物，具有抗氧化、抗炎和细胞保护作用。本研究评估了 DADS 对雄性大鼠镉生殖毒性的保护作用，强调了氧化还原失衡、TLR-4/NF-κB 和 JAK1/STAT3 信号以及 SIRT1 的参与：方法：给大鼠口服 DADS（10 毫克/千克体重）14 天，并在第 7 天腹腔注射单剂量镉（1.2 毫克/千克）。采集血液和睾丸样本进行分析：结果：镉造成的睾丸损伤表现为多种组织病理变化和曲细精管中精子的丧失。服用镉的大鼠体内促性腺激素和睾酮的循环水平下降。DADS 可预防镉引起的睾丸损伤，并改善血清中促性腺激素和睾酮的水平。镉增加了睾丸的活性氧（ROS）和丙二醛（MDA），并上调了TLR-4、NF-κB、促炎细胞因子、JAK1和STAT3磷酸化、Bax和caspase-3，同时降低了抗氧化剂和Bcl-2。DADS 能有效降低 ROS 和 MDA，下调 TLR-4、NF-κB、JAK1、STAT3、促炎细胞因子和促细胞凋亡标志物。此外，DADS 还能增强镉注射大鼠睾丸中的抗氧化剂、Bcl-2、SIRT1 和细胞色素：结论：DADS可通过减轻氧化应激、细胞凋亡、TLR-4/NF-κB和JAK1/STAT3信号传导，以及上调SIRT1和抗氧化剂来预防镉诱导的睾丸损伤。

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Diallyl disulfide prevents cadmium-induced testicular injury by attenuating oxidative stress, apoptosis, and TLR-4/NF-κB and JAK1/STAT3 signaling and upregulating SIRT1 in rats

Background

Cadmium (Cd) is a heavy metal environmental pollutant that can cause serious health problems. Cd can cause structural changes in the testes and exposure to this heavy metal is associated with the loss of sperms and male infertility. The role of oxidative stress and inflammation in Cd toxicity has been acknowledged. Diallyl disulfide (DADS), an organo-sulfur compound found in garlic, possesses antioxidant, anti-inflammatory, and cytoprotective effects. This study evaluated the protective effect of DADS against Cd reproductive toxicity in male rats, emphasizing the involvement of redox imbalance, TLR-4/NF-κB and JAK1/STAT3 signaling, and SIRT1.

Methods

DADS (10 mg/kg body weight) was administered orally to rats for 14 days and a single dose of Cd (1.2 mg/kg) was injected intraperitoneally on day 7. Blood and samples from the testes were collected for analysis.

Results

Cd caused testicular injury manifested by multiple histopathological changes and loss of sperms from seminiferous tubules. Circulating levels of gonadotropins and testosterone were decreased in Cd-administered rats. DADS prevented Cd-induced testicular injury and ameliorated serum levels of gonadotropins and testosterone. Cd increased testicular reactive oxygen species (ROS) and malondialdehyde (MDA) and upregulated TLR-4, NF-κB, pro-inflammatory cytokines, JAK1 and STAT3 phosphorylation, Bax and caspase-3, while decreased antioxidants and Bcl-2. DADS effectively decreased ROS and MDA, downregulated TLR-4, NF-κB, JAK1, STAT3, pro-inflammatory cytokines and pro-apoptosis markers in Cd-administered rats. In addition, DADS enhanced antioxidants, Bcl-2, SIRT1 and cytoglobin in the testis of Cd-administered rats.

Conclusion

DADS prevents Cd-induced testicular injury by attenuating oxidative stress, apoptosis, and TLR-4/NF-κB and JAK1/STAT3 signaling, and upregulating SIRT1 and antioxidants.

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来源期刊

Journal of Trace Elements in Medicine and Biology 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.90%

发文量

202

审稿时长

85 days

期刊介绍： The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.