利用气液界面模型评估无定形纳米塑料在气道和肺上皮细胞中的毒性。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere Pub Date : 2024-11-01 DOI:10.1016/j.chemosphere.2024.143702

I.F. Gosselink , F.J. van Schooten , M.J. Drittij , E.M. Höppener , P. Leonhardt , E. Moschini , T. Serchi , A.C. Gutleb , I.M. Kooter , A.H. Remels

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引用次数: 0

摘要

背景：吸入是纳米塑料的主要接触途径之一。有关纳米塑料对气道和肺上皮细胞毒理影响的知识十分有限，而且几乎完全基于使用球形聚苯乙烯（PS）颗粒的浸没体外模型：方法：将人类支气管和肺泡上皮细胞的单培养物和高级（共）培养物（均为气液界面（ALI）培养物）暴露于纳米塑料和参考纳米粒子。肺泡模型包括 A549 单培养物和与内皮细胞（Ea.hy926）和巨噬细胞样细胞（分化的 THP-1）共培养的 A549 细胞。支气管模型包括 BEAS-2B 细胞和分化的原发性支气管上皮细胞（PBEC）。培养物暴露于 PS、氧化铜（II）（CuO）或二氧化钛（TiO2）纳米颗粒（50 nm）。此外，BEAS-2B 细胞还接触了特性良好的无定形聚氯乙烯（PVC）、聚丙烯（PP）或聚酰胺（PA）纳米塑料。暴露 24 小时后，对细胞毒性和炎症（IL-8 分泌和 IL-8 转录水平）进行评估：结果：在所有模型中，细胞活力不受所有暴露的影响。与 PS 和 TiO2 不同，CuO 暴露剂量依赖性地诱导 IL-8 蛋白分泌和 mRNA 水平。虽然不同模型分泌 IL-8 的程度不同，但在单培养基和高级（共）培养基中，对 CuO 的相对反应相似。与环境相关的纳米塑料（PVC、PA 或 PP）都不会影响 BEAS-2B ALI 培养物中的炎症或细胞活力：结论：尽管氧化铜会诱发炎症，但 PS 在我们的任何模型中都未能引起炎症反应。我们首次证明，在 BEAS-2B ALI 模型中，PVC、PA 和 PP 不会诱导细胞死亡或炎症反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Assessing toxicity of amorphous nanoplastics in airway- and lung epithelial cells using air-liquid interface models

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Assessing toxicity of amorphous nanoplastics in airway- and lung epithelial cells using air-liquid interface models

Background

Inhalation is one of the main exposure routes to nanoplastics. Knowledge of the toxicological impact of nanoplastics on the airway- and lung epithelium is limited and almost exclusively based on submerged in vitro models using spherical polystyrene (PS) particles.

Methods

Mono-cultures and advanced (co-)cultures of human bronchial- and alveolar epithelial cells, all air-liquid interface (ALI) cultures, were exposed to nanoplastics and reference nanoparticles. Alveolar models included A549 mono-cultures and A549 cells co-cultured with endothelial cells (Ea.hy926) and macrophage-like cells (differentiated THP-1). Bronchial models included BEAS-2B cells and differentiated primary bronchial epithelial cells (PBEC). Cultures were exposed to PS, copper(II) oxide (CuO) or titanium dioxide (TiO₂) nanoparticles (50 nm). Additionally, BEAS-2B cells were exposed to well-characterised, amorphous polyvinyl chloride (PVC), polypropylene (PP), or polyamide (PA) nanoplastics. Cytotoxicity and inflammation (IL-8 secretion and IL-8 transcript levels) were assessed after 24 h of exposure.

Results

Cell viability remained unaffected by all exposures in all models. Unlike PS and TiO₂, CuO exposure dose-dependently induced IL-8 protein secretion and mRNA levels. Although the extent of IL-8 secretion differed between models, the relative response to CuO was similar in both mono-cultures and advanced (co-)cultures. None of the environmentally relevant nanoplastics (PVC, PA or PP) impacted inflammation or cell viability in BEAS-2B ALI cultures.

Conclusion

Although CuO induced inflammation, PS failed to elicit an inflammatory response in any of our models. For the first time, we show that PVC, PA and PP do not induce cell death or inflammation in a BEAS-2B ALI model.

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来源期刊

Chemosphere 环境科学-环境科学

CiteScore

15.80

自引率

8.00%

发文量

4975

审稿时长

3.4 months

期刊介绍： Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.