利用多重荧光免疫组化染色法测量反复种植失败妇女的四种子宫 NK 细胞亚型

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Lu Chen, Loucia Kit Ying Chan, Stacey Chui Yiu Wong, Joshua Jing Xi Li, Jacqueline Pui Wah Chung, Chi Chiu Wang, Mingqing Li, Tao Zhang
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引用次数: 0

摘要

免疫组化(IHC)在生物研究和临床诊断中发挥着至关重要的作用,是鉴定和观察组织抗原最常用的方法。然而,传统的 IHC 染色方法在区分各种亚型免疫细胞方面存在局限性。这一挑战促使科学家们探索新的技术和方法来精确鉴定和区分免疫细胞亚型。近年来,多重 IHC 已成为一种解决方案,它可以同时检测多种抗原,并在同一组织样本中将其可视化。子宫自然杀伤(uNK)细胞在早期妊娠过程中起着关键作用,包括蜕膜化、子宫螺旋动脉重塑和胚胎植入。不同亚型的uNK细胞表现出不同的功能,使它们能够协调各种生物事件,从而使胚胎发育和妊娠成功。因此,深入研究uNK细胞亚型对于阐明孕期免疫调节机制至关重要。这些研究为解决不孕症和复发性生殖失败等相关问题提供了有价值的见解和新方法。本文介绍了一种详细的多重 IHC 染色方案,用于研究植入窗口期(WOI)子宫内膜标本中四种亚型 uNK 细胞的密度。该方案包括样本制备、亚型标记优化、显微成像和数据分析。这种多重 IHC 染色方案具有高特异性和高灵敏度,能同时检测不同的 uNK 细胞亚型,从而为研究人员探索孕期免疫调节的复杂性和机制提供了强有力的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Measurement of Four Uterine NK Cell Subtypes Using Multiplexed Fluorescent Immunohistochemical Staining in Women with Repeated Implantation Failure.

Immunohistochemistry (IHC) plays a crucial role in biological research and clinical diagnosis, serving as the most commonly used method for identifying and visualizing tissue antigens. However, traditional IHC staining methods have limitations in distinguishing various subtypes of immune cells. This challenge has driven scientists to explore new technologies and methodologies for precise identification and differentiation of immune cell subtypes. In recent years, multiplex IHC has emerged as a solution, enabling the simultaneous detection of multiple antigens and their visualization within the same tissue sample. Uterine natural killer (uNK) cells play a pivotal role in early pregnancy processes, including decidualization, remodeling of uterine spiral arteries, and embryo implantation. Different subtypes of uNK cells exhibit different functions, allowing them to coordinate various biological events for successful embryo development and pregnancy. Therefore, in-depth research on uNK cell subtypes is essential for elucidating immune regulation mechanisms during pregnancy. Such studies provide valuable insights and novel approaches for addressing related conditions such as infertility and recurrent reproductive failure. This paper introduces a detailed multiplex IHC staining protocol for studying the density of four subtypes of uNK cells in endometrial specimens during the window of implantation (WOI). The protocol includes sample preparation, optimization of subtype markers, microscopic imaging, and data analyses. This multiplex IHC staining protocol offers high specificity and sensitivity, enabling simultaneous detection of different uNK cell subtypes, thus providing researchers with a powerful tool to explore the intricacies and mechanisms of immune regulation during pregnancy.

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来源期刊
Jove-Journal of Visualized Experiments
Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-
CiteScore
2.10
自引率
0.00%
发文量
992
期刊介绍: JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.
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