乌干达北部帕德尔地区用于治疗念珠菌病的药用植物的体外抗真菌活性。

IF 3.6 Q1 TROPICAL MEDICINE
Betty Akwongo, Esezah K Kakudidi, Anthony M Nsubuga, Morgan Andama, Mary Namaganda, Patience Tugume, Savina Asiimwe, Godwin Anywar, Esther Katuura
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Extracts and their combinations, positive controls (amphotericin B, and fluconazole) and negative control (80% dimethyl sulfoxide, verified to be tolerable concentration to the tested Candida species) were screened and verified for their antifungal activity against Candida albicans (ATCC: American Type Culture Collection reference strain 10231, ATCC 90028, 0770a and 0796), C. glabrata (VVc 004, ATCC 2950) and C. tropicalis (ATCC 750 and 0210) using agar well diffusion and broth micro-dilution, respectively.</p><p><strong>Results: </strong>Aqueous extract (24.4 °C) of M. rubrostipulata (ZOI: 18.00 ± 1.00 to 38.33 ± 0.17; MIC: 3.13 ± 0.00 to 20.83 ± 4.17; MFC: 12.50 ± 0.00 to 200.00 ± 0.00), methanol extract of K. anthotheca (10.11 ± 0.31 to 15.11 ± 0.65; 1.04 ± 0.26 to 12.50 ± 0.00; 12.50 ± 0.00 to 100.00 ± 0.00), and combination of aqueous extract (60 °C) of D. dissectus + methanol extract of K. anthotheca (7.89 ± 0.26 to 19.67 ± 0.37; 0.78 ± 0.00 to 50.00 ± 0.00; 12.50 ± 0.00 to 200.00 ± 0.00) exhibited broad spectrum antifungal activities and were fungistatic against all tested Candida species, which comprised 8 clinical/control and susceptible/resistant strains. 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引用次数: 0

摘要

背景:由于出现了对现有药物具有多重耐药性的念珠菌,全球范围内对草药的使用重新产生了兴趣。本研究科学地验证了帕德尔地区五种常用植物对部分致病念珠菌菌株的抗真菌效果:方法:分别使用石油醚和甲醇,以及 24.4 °C(浸泡)、60 °C(煎煮)和 87 °C(热水浸泡)的全水萃取法,对 Momordica foetida、Sansevieria dawei 和 Distimake dissectus 的粉末根茎,以及 Khaya anthotheca 和 Mitragyna rubrostipulata 的茎皮进行提取。筛选并验证了提取物及其组合、阳性对照(两性霉素 B 和氟康唑)和阴性对照(80% 的二甲亚砜,经证实为受试念珠菌的耐受浓度)对白色念珠菌(ATCC:美国模式培养物保藏中心参考菌株 10231、ATCC 90028、0770a 和 0796)、C.分别使用琼脂井扩散法和肉汤微量稀释法对白色念珠菌(ATCC:美国类型培养物保藏中心参考菌株 10231、ATCC 90028、0770a 和 0796)、白色念珠菌(VVc 004、ATCC 2950)和热带念珠菌(ATCC 750 和 0210)进行抗菌:结果:M. rubrostipulata 的水提取物(24.4 °C)(ZOI:18.00 ± 1.00 至 38.33 ± 0.17;MIC:3.13 ± 0.00 至 20.83 ± 4.17;MFC:12.50 ± 0.00 至 200.00 ± 0.00)、K.anthotheca的甲醇提取物(10.11 ± 0.31 至 15.11 ± 0.65;1.04 ± 0.26 至 12.50 ± 0.00;12.50 ± 0.00 至 100.00 ± 0.00),以及 D. dissectus的水提取物(60 °C)+甲醇提取物的组合。D. dissectus + K. anthotheca 的甲醇提取物(7.89 ± 0.26 至 19.67 ± 0.37;0.78 ± 0.00 至 50.00 ± 0.00;12.50 ± 0.00 至 200.00 ± 0.00)的组合具有广谱抗真菌活性,对所有测试的念珠菌菌种(包括 8 个临床/对照菌株和易感/耐药菌株)均有杀真菌作用。在所有测试的念珠菌种类/菌株中,没有一种常规药物具有广谱抗真菌活性:结论:K. anthotheca 的甲醇提取物、M. rubrostipulata 的水提取物(24.4 °C)以及 D. dissectus 的水提取物(60 °C)+ K. anthotheca 的甲醇提取物的组合可有效治疗念珠菌病。与氟康唑和两性霉素 B 相比,它们对不同种类和菌株的念珠菌具有潜在的广谱抗真菌活性。它们的杀真菌性表明了它们抑制真菌生长的能力。因此,如果对这些提取物/提取物组合进行标准化,并将其活性治疗化合物分离出来制成抗真菌药物,就能为念珠菌病提供更好的治疗选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro antifungal activities of medicinal plants used for treatment of candidiasis in Pader district, Northern Uganda.

Background: The emergence of multidrug resistant Candida species to available drugs has led to renewed interest in the use of herbal medicines globally. This study scientifically verified antifungal effectiveness of five commonly used plant species in Pader district, against selected pathogenic candida strains.

Methods: Powdered roots of Momordica foetida, Sansevieria dawei and Distimake dissectus; and stem barks of Khaya anthotheca and Mitragyna rubrostipulata were extracted sequentially using petroleum ether and methanol, respectively; and total water extraction at 24.4 °C (maceration), 60 °C (decoction) and boiling water at 87 °C (hot water infusion). Extracts and their combinations, positive controls (amphotericin B, and fluconazole) and negative control (80% dimethyl sulfoxide, verified to be tolerable concentration to the tested Candida species) were screened and verified for their antifungal activity against Candida albicans (ATCC: American Type Culture Collection reference strain 10231, ATCC 90028, 0770a and 0796), C. glabrata (VVc 004, ATCC 2950) and C. tropicalis (ATCC 750 and 0210) using agar well diffusion and broth micro-dilution, respectively.

Results: Aqueous extract (24.4 °C) of M. rubrostipulata (ZOI: 18.00 ± 1.00 to 38.33 ± 0.17; MIC: 3.13 ± 0.00 to 20.83 ± 4.17; MFC: 12.50 ± 0.00 to 200.00 ± 0.00), methanol extract of K. anthotheca (10.11 ± 0.31 to 15.11 ± 0.65; 1.04 ± 0.26 to 12.50 ± 0.00; 12.50 ± 0.00 to 100.00 ± 0.00), and combination of aqueous extract (60 °C) of D. dissectus + methanol extract of K. anthotheca (7.89 ± 0.26 to 19.67 ± 0.37; 0.78 ± 0.00 to 50.00 ± 0.00; 12.50 ± 0.00 to 200.00 ± 0.00) exhibited broad spectrum antifungal activities and were fungistatic against all tested Candida species, which comprised 8 clinical/control and susceptible/resistant strains. None of the conventional drugs used demonstrated broad spectrum antifungal activity across all tested Candida species/strains.

Conclusion: Methanol extract of K. anthotheca, aqueous extract (24.4 °C) of M. rubrostipulata, and combination of aqueous extract (60 °C) of D. dissectus + methanol extract of K. anthotheca could be effective in the treatment of candidiasis. They demonstrated potential broad spectrum antifungal activity against different species and strains of tested Candida than the fluconazole and amphotericin B drugs. Their fungistatic nature showed their ability to inhibit fungal growth. Hence, these extracts/extract combination can offer better treatment option for candidiasis if they are standardized and also their active curative compounds isolated and made into antifungal drugs.

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来源期刊
Tropical Medicine and Health
Tropical Medicine and Health TROPICAL MEDICINE-
CiteScore
7.00
自引率
2.20%
发文量
90
审稿时长
11 weeks
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