计算点扩散函数:方法、陷阱和解决方案。

IF 3.2 2区 物理与天体物理 Q2 OPTICS
Optics express Pub Date : 2024-07-29 DOI:10.1364/OE.523532
Ratsimandresy Holinirina Dina Miora, Erich Rohwer, Martin Kielhorn, Colin Sheppard, Gurthwin Bosman, Rainer Heintzmann
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引用次数: 0

摘要

了解光学系统点扩散函数(PSF)的确切结构,可以在荧光显微镜中重建高质量的图像。精确的 PSF 模型考虑了光的矢量性质以及相位和振幅修正。现有的大多数基于实空间的 PSF 模型都会在中心位置附近陷入采样误区,从而违反能量守恒。在这项工作中,我们提出了一种新颖的、据我们所知基于傅立叶的矢量 PSF 计算技术,并将其与最先进的技术进行了比较。我们的方法符合成像过程的物理条件。这些方法具有可重复性、计算效率高、易于实现、易于修改以表示各种成像模式。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Calculating point spread functions: methods, pitfalls, and solutions.

The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the center position, yielding to the violation of energy conservation. In this work, we present a novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, easy to implement, and easy to modify to represent various imaging modalities.

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来源期刊
Optics express
Optics express 物理-光学
CiteScore
6.60
自引率
15.80%
发文量
5182
审稿时长
2.1 months
期刊介绍: Optics Express is the all-electronic, open access journal for optics providing rapid publication for peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.
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