OLP 病变中的 HSP90 复合物通过激活树突状细胞诱导 T 细胞极化

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2024-11-12 DOI:10.1111/odi.15195

Lei Pan, Junjun Chen, Yirao Lai, Guanhuan Du, Haiyan Wang, Lei Sun, Guoyao Tang, Yufeng Wang

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引用次数: 0

摘要

研究目的研究口腔扁平苔藓（OLP）病变组织中的热休克蛋白90（HSP90）复合物对树突状细胞（DC）活化和幼稚T细胞极化的影响：方法：通过单细胞 RNA 序列、IHC、qRT-PCR 和免疫印迹法评估 HSP90 在 OLP 病变和健康对照（HC）粘膜中的表达。通过免疫沉淀从口腔黏膜中提取 HSP90 复合物作为 DCs 的激动剂。流式细胞术检测 IFN-α 和 MHC 的表达。与预刺激的 DCs 共培养后，通过细胞因子分析研究了幼稚 T 细胞的极化：结果：HSP90在OLP病变的固有层中明显升高，且与淋巴细胞浸润密切相关。OLP 病变的 HSP90 复合物通过 TLR9 激活 DCs，增加其 IFN-α 分泌和 MHC II 表达。预刺激的DC增加了Th17细胞的比例：结论：从OLP病变中分离出的HSP90复合物可激活DCs的TLR9/IFN-α，并进一步促进幼稚T细胞向Th17免疫极化。

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HSP90 Complex From OLP Lesion Induces T-Cell Polarization via Activation of Dendritic Cells.

Objectives: To investigate the effects of the heat shock protein 90 (HSP90) complex from oral lichen planus (OLP) lesion tissues on dendritic cell (DC) activation and the polarization of naïve T cells.

Methods: Expression of HSP90 in OLP lesions and healthy control (HC) mucosa was evaluated by single-cell RNA sequence, IHC, qRT-PCR, and immunoblotting. HSP90 complex was extracted by immunoprecipitation from oral mucosa as the agonist of DCs. Expression of IFN-α and MHC was detected by flow cytometry. After cocultured with pre-stimulated DCs, polarization of naïve T cells was investigated by cytokine analysis.

Results: HSP90 was significantly higher in the lamina propria of OLP lesion and closely related to lymphocyte infiltration. HSP90 complex of OLP lesion activated DCs via TLR9 and increased their IFN-α secretion and MHC II expression. Pre-stimulated DCs increased the proportion of Th17 cells.

Conclusions: HSP90 complex isolated from OLP lesion activated TLR9/IFN-α of DCs and further promoted the polarization of naïve T cells toward Th17 immunity.

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.