用白炭黑绿和高山试剂鉴别家猫(Felis catus)的不同血液浓度。

IF 0.9 Q3 VETERINARY SCIENCES
Open Veterinary Journal Pub Date : 2024-10-01 Epub Date: 2024-10-31 DOI:10.5455/OVJ.2024.v14.i10.10
Suraida Meisari, Tita Damayanti Lestari, Djoko Legowo, Thomas Valentinus Widiyatno, Eka Pramyrtha Hestianah, Retno Sri Wahjuni, Ratna Damayanti, Aswin Rafif Khairullah, Ikechukwu Benjamin Moses, Ricadonna Raissa
{"title":"用白炭黑绿和高山试剂鉴别家猫(Felis catus)的不同血液浓度。","authors":"Suraida Meisari, Tita Damayanti Lestari, Djoko Legowo, Thomas Valentinus Widiyatno, Eka Pramyrtha Hestianah, Retno Sri Wahjuni, Ratna Damayanti, Aswin Rafif Khairullah, Ikechukwu Benjamin Moses, Ricadonna Raissa","doi":"10.5455/OVJ.2024.v14.i10.10","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Cases of cruelty can occur in wild animal, livestock, and pet animal. Cruelty to cats choose to be the background of this research because many cases of cruelty to cats have not been reported although the cases are still high.</p><p><strong>Aim: </strong>The aim of this research was to know how much dilution of cat blood can still be detected by Leucomalachite Green (LMG) and Takayama reagent.</p><p><strong>Methods: </strong>Three samples of domestic cat blood were diluted with ratios 1:10; 1:100; 1:1,000; 1:10,000; and 1:100,000. Bloodstain is made by dropping each blood dilution on the filter paper for the LMG test and on the object glass for the Takayama test. Bloodstain for the LMG test was done with ten repetitions from each sample and Duplo for the Takayama test. Positive results from the LMG test are presented as a bluish-green discoloration in stains. A positive result from the Takayama test is the formation of hemochromogen crystals under a microscope with 400× magnification.</p><p><strong>Results: </strong>Based on this research, cat bloodstain can be detected with LMG reagent until 1:40,000 dilution, while Takayama reagent only can detect cat bloodstain until 1:1,000.</p><p><strong>Conclusion: </strong>LMG and Takayama reagents are reagents that are often used in human blood spot testing. If there is a case of violence against cats and other animals, these two reagents can be relied on to help with the proof process.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 10","pages":"2609-2617"},"PeriodicalIF":0.9000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11560260/pdf/","citationCount":"0","resultStr":"{\"title\":\"Identification of different blood concentration of domestic cat (<i>Felis catus</i>) with Leucomalachite Green and Takayama reagent.\",\"authors\":\"Suraida Meisari, Tita Damayanti Lestari, Djoko Legowo, Thomas Valentinus Widiyatno, Eka Pramyrtha Hestianah, Retno Sri Wahjuni, Ratna Damayanti, Aswin Rafif Khairullah, Ikechukwu Benjamin Moses, Ricadonna Raissa\",\"doi\":\"10.5455/OVJ.2024.v14.i10.10\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Cases of cruelty can occur in wild animal, livestock, and pet animal. Cruelty to cats choose to be the background of this research because many cases of cruelty to cats have not been reported although the cases are still high.</p><p><strong>Aim: </strong>The aim of this research was to know how much dilution of cat blood can still be detected by Leucomalachite Green (LMG) and Takayama reagent.</p><p><strong>Methods: </strong>Three samples of domestic cat blood were diluted with ratios 1:10; 1:100; 1:1,000; 1:10,000; and 1:100,000. Bloodstain is made by dropping each blood dilution on the filter paper for the LMG test and on the object glass for the Takayama test. Bloodstain for the LMG test was done with ten repetitions from each sample and Duplo for the Takayama test. Positive results from the LMG test are presented as a bluish-green discoloration in stains. A positive result from the Takayama test is the formation of hemochromogen crystals under a microscope with 400× magnification.</p><p><strong>Results: </strong>Based on this research, cat bloodstain can be detected with LMG reagent until 1:40,000 dilution, while Takayama reagent only can detect cat bloodstain until 1:1,000.</p><p><strong>Conclusion: </strong>LMG and Takayama reagents are reagents that are often used in human blood spot testing. If there is a case of violence against cats and other animals, these two reagents can be relied on to help with the proof process.</p>\",\"PeriodicalId\":19531,\"journal\":{\"name\":\"Open Veterinary Journal\",\"volume\":\"14 10\",\"pages\":\"2609-2617\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11560260/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Veterinary Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/OVJ.2024.v14.i10.10\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/31 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/OVJ.2024.v14.i10.10","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/31 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

背景:野生动物、家畜和宠物动物都可能受到虐待。研究目的:本研究旨在了解稀释多少的猫血仍能被白腊石绿(LMG)和高山试剂检测到:方法:将三份家猫血液样本按 1:10、1:100、1:1,000、1:10,000 和 1:100,000 的比例稀释。在进行 LMG 检验时,将每份血液稀释液滴在滤纸上,在进行 Takayama 检验时,将每份血液稀释液滴在物镜上。在 LMG 检验中,每个样本的血迹要重复十次;在高山检验中,每个样本的血迹要重复十次。LMG 检验的阳性结果显示为污渍呈蓝绿色变色。高山试验的阳性结果是在放大 400 倍的显微镜下形成血色素晶体:根据这项研究,LMG 试剂可检测到 1:40,000 稀释度的猫血迹,而高山试剂只能检测到 1:1,000 稀释度的猫血迹:LMG 试剂和高山试剂是人体血迹检测中常用的试剂。如果发生了针对猫和其他动物的暴力案件,这两种试剂可以帮助进行证明。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent.

Background: Cases of cruelty can occur in wild animal, livestock, and pet animal. Cruelty to cats choose to be the background of this research because many cases of cruelty to cats have not been reported although the cases are still high.

Aim: The aim of this research was to know how much dilution of cat blood can still be detected by Leucomalachite Green (LMG) and Takayama reagent.

Methods: Three samples of domestic cat blood were diluted with ratios 1:10; 1:100; 1:1,000; 1:10,000; and 1:100,000. Bloodstain is made by dropping each blood dilution on the filter paper for the LMG test and on the object glass for the Takayama test. Bloodstain for the LMG test was done with ten repetitions from each sample and Duplo for the Takayama test. Positive results from the LMG test are presented as a bluish-green discoloration in stains. A positive result from the Takayama test is the formation of hemochromogen crystals under a microscope with 400× magnification.

Results: Based on this research, cat bloodstain can be detected with LMG reagent until 1:40,000 dilution, while Takayama reagent only can detect cat bloodstain until 1:1,000.

Conclusion: LMG and Takayama reagents are reagents that are often used in human blood spot testing. If there is a case of violence against cats and other animals, these two reagents can be relied on to help with the proof process.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Open Veterinary Journal
Open Veterinary Journal VETERINARY SCIENCES-
CiteScore
1.40
自引率
0.00%
发文量
112
审稿时长
12 weeks
期刊介绍: Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信