{"title":"Akkermansia muciniphila 促进早期断奶仔猪 SIgA 的产生并改变其对共生细菌的反应性","authors":"Qin Jiang, Xiaoyan Zhu, Lingling Sun, Chunlin Xie, Xinkai Wang, Libao Ma, Xianghua Yan","doi":"10.1016/j.tjnut.2024.11.002","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Secretory IgA (SIgA) is the first line of defense in protecting the intestinal epithelium against pathogenic bacteria, regulating gut microbiota composition, and maintaining intestinal homeostasis. Early weaning strategies may disrupt SIgA levels in piglet intestines, causing a decline in immune response and early weaning stress. However, the specific microbial mechanisms modulating SIgA in early-weaned piglets are not well understood.</p><p><strong>Objective: </strong>We hypothesized that Akkermansia muciniphila increases intestinal SIgA production in the early-weaned piglets.</p><p><strong>Methods: </strong>Fecal SIgA levels, SIgA-coated bacteria abundance, and fecal metagenomes were compared between 6 Huanjiang miniature (HM) and 6 Duroc×Landrace×Yorkshire (DLY) early-weaned piglets to identify bacterial species involved in SIgA modulation. 4 bacterial species were investigated using 5 groups (Control, A. muciniphila, L. amylovorus, L. crispatus, L. acidophilus) of male SPF C57BL/6J mice, weaned 3 weeks post-birth (n=8/group). Subsequently, 10-day-old Landrace×Yorkshire (LY) piglets were randomly assigned to three groups (Control, 10<sup>9</sup>A. muciniphila, 10<sup>8</sup>A. muciniphila) (n=10/group) to evaluate the effect of orally administered A. muciniphila on intestinal SIgA production and microbial composition.</p><p><strong>Results: </strong>HM early-weaned piglets showed significantly higher SIgA levels (7.59 μg/mg, 95% CI: 3.2, 12, P = 0.002) and SIgA-coated bacteria abundance (8.64%, 95% CI: 3.2, 14, P = 0.014) than DLY piglets. In the mouse model, administration of A. muciniphila significantly increased SIgA levels (3.50 μg/mg, 95% CI: 0.59, 6.4, P = 0.018), SIgA-coated bacteria abundance (9.06%, 95% CI: 4, 14, P =0.018), and IgA<sup>+</sup> plasma cell counts (6.1%, 95% CI: 4.3, 8, P = 0.005). In the pig experiments, oral administration of A. muciniphila to LY piglets significantly enhanced intestinal SIgA concentrations (4.22μg/mg, 95% CI: 0.37, 8.5, P = 0.034) and altered the SIgA-coated bacterial landscape.</p><p><strong>Conclusion: </strong>Early intervention with A. muciniphila in nursing piglets can increases intestinal SIgA productionand alter the reactivity towards commensal bacteria upon early weaning.</p>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":" ","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Akkermansia muciniphila Promotes SIgA Production and Alters the Reactivity Towards Commensal Bacteria in Early-Weaned Piglets.\",\"authors\":\"Qin Jiang, Xiaoyan Zhu, Lingling Sun, Chunlin Xie, Xinkai Wang, Libao Ma, Xianghua Yan\",\"doi\":\"10.1016/j.tjnut.2024.11.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Secretory IgA (SIgA) is the first line of defense in protecting the intestinal epithelium against pathogenic bacteria, regulating gut microbiota composition, and maintaining intestinal homeostasis. Early weaning strategies may disrupt SIgA levels in piglet intestines, causing a decline in immune response and early weaning stress. However, the specific microbial mechanisms modulating SIgA in early-weaned piglets are not well understood.</p><p><strong>Objective: </strong>We hypothesized that Akkermansia muciniphila increases intestinal SIgA production in the early-weaned piglets.</p><p><strong>Methods: </strong>Fecal SIgA levels, SIgA-coated bacteria abundance, and fecal metagenomes were compared between 6 Huanjiang miniature (HM) and 6 Duroc×Landrace×Yorkshire (DLY) early-weaned piglets to identify bacterial species involved in SIgA modulation. 4 bacterial species were investigated using 5 groups (Control, A. muciniphila, L. amylovorus, L. crispatus, L. acidophilus) of male SPF C57BL/6J mice, weaned 3 weeks post-birth (n=8/group). Subsequently, 10-day-old Landrace×Yorkshire (LY) piglets were randomly assigned to three groups (Control, 10<sup>9</sup>A. muciniphila, 10<sup>8</sup>A. muciniphila) (n=10/group) to evaluate the effect of orally administered A. muciniphila on intestinal SIgA production and microbial composition.</p><p><strong>Results: </strong>HM early-weaned piglets showed significantly higher SIgA levels (7.59 μg/mg, 95% CI: 3.2, 12, P = 0.002) and SIgA-coated bacteria abundance (8.64%, 95% CI: 3.2, 14, P = 0.014) than DLY piglets. In the mouse model, administration of A. muciniphila significantly increased SIgA levels (3.50 μg/mg, 95% CI: 0.59, 6.4, P = 0.018), SIgA-coated bacteria abundance (9.06%, 95% CI: 4, 14, P =0.018), and IgA<sup>+</sup> plasma cell counts (6.1%, 95% CI: 4.3, 8, P = 0.005). In the pig experiments, oral administration of A. muciniphila to LY piglets significantly enhanced intestinal SIgA concentrations (4.22μg/mg, 95% CI: 0.37, 8.5, P = 0.034) and altered the SIgA-coated bacterial landscape.</p><p><strong>Conclusion: </strong>Early intervention with A. muciniphila in nursing piglets can increases intestinal SIgA productionand alter the reactivity towards commensal bacteria upon early weaning.</p>\",\"PeriodicalId\":16620,\"journal\":{\"name\":\"Journal of Nutrition\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-11-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nutrition\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.tjnut.2024.11.002\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutrition","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.tjnut.2024.11.002","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
Akkermansia muciniphila Promotes SIgA Production and Alters the Reactivity Towards Commensal Bacteria in Early-Weaned Piglets.
Background: Secretory IgA (SIgA) is the first line of defense in protecting the intestinal epithelium against pathogenic bacteria, regulating gut microbiota composition, and maintaining intestinal homeostasis. Early weaning strategies may disrupt SIgA levels in piglet intestines, causing a decline in immune response and early weaning stress. However, the specific microbial mechanisms modulating SIgA in early-weaned piglets are not well understood.
Objective: We hypothesized that Akkermansia muciniphila increases intestinal SIgA production in the early-weaned piglets.
Methods: Fecal SIgA levels, SIgA-coated bacteria abundance, and fecal metagenomes were compared between 6 Huanjiang miniature (HM) and 6 Duroc×Landrace×Yorkshire (DLY) early-weaned piglets to identify bacterial species involved in SIgA modulation. 4 bacterial species were investigated using 5 groups (Control, A. muciniphila, L. amylovorus, L. crispatus, L. acidophilus) of male SPF C57BL/6J mice, weaned 3 weeks post-birth (n=8/group). Subsequently, 10-day-old Landrace×Yorkshire (LY) piglets were randomly assigned to three groups (Control, 109A. muciniphila, 108A. muciniphila) (n=10/group) to evaluate the effect of orally administered A. muciniphila on intestinal SIgA production and microbial composition.
Results: HM early-weaned piglets showed significantly higher SIgA levels (7.59 μg/mg, 95% CI: 3.2, 12, P = 0.002) and SIgA-coated bacteria abundance (8.64%, 95% CI: 3.2, 14, P = 0.014) than DLY piglets. In the mouse model, administration of A. muciniphila significantly increased SIgA levels (3.50 μg/mg, 95% CI: 0.59, 6.4, P = 0.018), SIgA-coated bacteria abundance (9.06%, 95% CI: 4, 14, P =0.018), and IgA+ plasma cell counts (6.1%, 95% CI: 4.3, 8, P = 0.005). In the pig experiments, oral administration of A. muciniphila to LY piglets significantly enhanced intestinal SIgA concentrations (4.22μg/mg, 95% CI: 0.37, 8.5, P = 0.034) and altered the SIgA-coated bacterial landscape.
Conclusion: Early intervention with A. muciniphila in nursing piglets can increases intestinal SIgA productionand alter the reactivity towards commensal bacteria upon early weaning.
期刊介绍：
The Journal of Nutrition (JN/J Nutr) publishes peer-reviewed original research papers covering all aspects of experimental nutrition in humans and other animal species; special articles such as reviews and biographies of prominent nutrition scientists; and issues, opinions, and commentaries on controversial issues in nutrition. Supplements are frequently published to provide extended discussion of topics of special interest.
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