破译李斯特菌对过氧乙酸反应的分子机制。

IF 2.1 4区农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of food protection Pub Date : 2024-11-07 DOI:10.1016/j.jfp.2024.100401

Marianna Arvaniti , Ahmed Gaballa , Renato H. Orsi , Panagiotis Skandamis , Martin Wiedmann

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引用次数: 0

摘要

过氧乙酸（PAA）是一种强氧化剂，由于不会产生有害的氯化副产物，因此被广泛用作食品加工过程中的消毒剂。本研究利用 RNA 序列分析（RNA-seq）评估了单核细胞增生李斯特菌对亚致死浓度 PAA（2.5 ppm）的转录反应。与对照样本相比，我们的分析发现 PAA 处理样本中有 12 个蛋白编码基因表达不同，其中 9 个基因上调（ohrR、ohrA、rpsN、lmo0637、lmo1973、fur、lmo2492、zurM 和 lmo1007），3 个基因下调（argG、lmo0604 和 lmo2156）。此外，还发现一个非编码小 RNA 基因（rli32）被下调。具体而言，单核细胞增多性乳酸菌暴露于 PAA 时，有机过氧化物毒性保护（OhrA-OhrR）系统、金属稳态基因 fur 和 zurM、SbrE 调控的 lmo0636-lmo0637 操作子和碳水化合物磷酸转移酶系统（PTS）操作子成分被诱导。因此，这项研究确定了单核细胞增多性乳酸菌对 PAA 引起的氧化应激的主要反应所涉及的关键因素，包括过氧化物解毒系统的表达和细胞中氧化还原活性金属水平的微调。研究单核细胞增生梭菌对 PAA 反应的分子机制对食品工业至关重要，因为残留 PAA 可导致病原体的应激耐受性。

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Deciphering the Molecular Mechanism of Peracetic Acid Response in Listeria monocytogenes

Peracetic acid (PAA), a strong oxidizing agent, has been widely used as a disinfectant in food processing settings as it does not produce harmful chlorinated by-products. In the present study, the transcriptional response of Listeria monocytogenes to a sub-lethal concentration of PAA (2.5 ppm) was assessed using RNA-sequencing (RNA-seq). Our analysis revealed 12 differentially expressed protein-coding genes, of which nine were upregulated (ohrR, ohrA, rpsN, lmo0637, lmo1973, fur, lmo2492, zurM, and lmo1007), and three were down-regulated (argG, lmo0604 and lmo2156) in PAA-treated samples compared to the control samples. A non-coding small RNA gene (rli32) was also found to be down-regulated. In detail, the organic peroxide toxicity protection (OhrA-OhrR) system, the metal homeostasis genes fur and zurM, the SbrE-regulated lmo0636-lmo0637 operon and a carbohydrate phosphotransferase system (PTS) operon component were induced under exposure of L. monocytogenes to PAA. Hence, this study identified key elements involved in the primary response of L. monocytogenes to oxidative stress caused by PAA, including the expression of the peroxide detoxification system and fine-tuning the levels of redox-active metals in the cell. The investigation of the molecular mechanism of PAA response in L. monocytogenes is of utmost importance for the food industry, as residual PAA can lead to stress tolerance in pathogens.

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来源期刊

Journal of food protection 工程技术-生物工程与应用微生物

CiteScore

4.20

自引率

5.00%

发文量

296

审稿时长

2.5 months

期刊介绍： The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with: Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain; Microbiological food quality and traditional/novel methods to assay microbiological food quality; Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation; Food fermentations and food-related probiotics; Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers; Risk assessments for food-related hazards; Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods; Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.