利用 HPLC-ELSD 和 HPLC-MS 对瘘管属精液中的甾体皂苷进行定量测定

IF 2.3 3区化学 Q3 CHEMISTRY, ANALYTICAL

Journal of Analytical Methods in Chemistry Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI:10.1155/2024/1872918

Zhancai Zheng, Deduo Xu, Wenquan Lu, Wansheng Chen, Yingbo Yang, Zhijun Wu

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引用次数: 0

摘要

本研究采用高效液相色谱-蒸发光散射检测法（HPLC-ELSD）和高效液相色谱-质谱法（HPLC-MS）对苁蓉中的甾体皂苷进行了定量分析。HPLC-ELSD方法简便、准确、重复性好，回收率为97.91%∼99.77%。同样，HPLC-MS 方法也具有快速、灵敏、准确和重复性好的特点，在对照样品中的回收率为 95.61%～99.70%。HPLC-MS 方法的灵敏度是 HPLC-ELSD 方法的数倍。这些结果共同表明，这些方法适用于苁蓉甾体皂苷含量的测定及其植物来源的鉴定。

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Quantitative Determination of Steroidal Saponins of Semen Allii Fistulosi Using HPLC-ELSD and HPLC-MS.

In this study, high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and HPLC-mass spectrometry (HPLC-MS) were employed for the quantitative analysis of steroidal saponins of Semen Allii Fistulosi. The HPLC-ELSD method was simple, accurate, and repeatable, and the recoveries were 97.91%∼99.77%. Similarly, the HPLC-MS method was rapid, sensitive, accurate, and reproducible, and the recoveries ranging from 95.61% to 99.70% in the control samples. The sensitivity of the HPLC-MS method was several times higher than that of the HPLC-ELSD method. These results collectively demonstrated that these methods are suitable for the content determination of steroidal saponins of Semen Allii Fistulosi and the identification of its plant source.

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来源期刊

Journal of Analytical Methods in Chemistry CHEMISTRY, ANALYTICAL-ENGINEERING, CIVIL

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Journal of Analytical Methods in Chemistry publishes papers reporting methods and instrumentation for chemical analysis, and their application to real-world problems. Articles may be either practical or theoretical. Subject areas include (but are by no means limited to): Separation Spectroscopy Mass spectrometry Chromatography Analytical Sample Preparation Electrochemical analysis Hyphenated techniques Data processing As well as original research, Journal of Analytical Methods in Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.