接受NOTES保胆手术治疗的慢性胆囊炎患者肠道微生物群的有益变化

IF 2 4区 医学 Q3 GASTROENTEROLOGY & HEPATOLOGY
Gastroenterology Research and Practice Pub Date : 2024-11-07 eCollection Date: 2024-01-01 DOI:10.1155/2024/9327118
Lixin Deng, Xinzhi Lv, Taotao Wang, Xishun Huang, Qingrong Huang, Xianli Li, Chunhong Wen, Li Chen, Huidi Chen, Mingqing Zhang
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引用次数: 0

摘要

目的:NOTES胆囊保留手术(N-GPS)被誉为慢性胆囊炎患者微创手术的新范式。本研究旨在评估 N-GPS 对患者肠道微生物群的影响。研究方法研究选择了术前 1 周内(BG_DPR 阶段)、术后随访 1 年以上(BG_YPO 阶段)的良性胆囊疾病患者(BG 组),并选择了性别、年龄和体重指数与 BG_YPO 阶段患者相匹配的健康对照组(HC 组)。因此,收集了健康对照组和两期良性胆囊疾病患者的粪便样本,并将其中的部分样本送至 Illumina MiSeq 平台进行 16S rDNA 测序,然后将合并样本送至 GC-MS 平台进行短链脂肪酸(SCFA)分析。结果香农指数的α多样性结果表明,BG组与HC组两个阶段的差异无统计学意义,而基于加权UniFrac距离的β多样性结果表明,BG组在YPO阶段的肠道微生物群结构更接近HC组。LEFSe分析表明,BG_YPO阶段富集了Enterocloster和Hungatella_A_128155等改善胆汁酸代谢的菌属。与 BG_DPR 阶段相比,BG_YPO 阶段和 HC 组富集了粪杆菌和 Roseburia,但减少了链球菌,同时粪便 SCFA 浓度增加。结论良性胆囊疾病和慢性胆囊炎患者经过一年以上的 N-GPS 治疗后,肠道微生物群落结构得到改善。随着胆汁酸代谢的改善,SCFA产生菌增加,病原菌减少,这有助于YPO阶段的BG组肠道微生物群落结构恢复并接近HC组。试验注册中国临床试验注册中心编号:ChiCTR1900028267:ChiCTR1900028267。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Beneficial Alterations of Intestinal Microbiota in Chronic Cholecystitis Patients Treated With NOTES Gallbladder-Preserving Surgery.

Objective: NOTES gallbladder-preserving surgery (N-GPS) has been heralded as a new paradigm shift in minimally invasive surgery for chronic cholecystitis patients. The objective of this research was to evaluate the impact of N-GPS on the intestinal microbiota of patients. Methods: The study selected patients with benign gallbladder disease (BG group) within 1 week preoperative (BG_DPR stage) and followed up over 1 year postoperative (BG_YPO stage) and selected healthy controls (HC group) whose sex, age, and BMI index matched with patients at BG_YPO stage, too. Accordingly, stool samples from healthy controls and two stages of patients with benign gallbladder disease were collected; among them, the selected samples were sent for 16S rDNA sequencing with Illumina MiSeq platform, and then, the combined samples were sent for short-chain fatty acid (SCFA) analysis with GC-MS platform. Results: The result of alpha diversity of Shannon index showed that the difference among the two stages of BG group and HC group wasn't statistically significant, while the result of beta diversity based on the weighted UniFrac distance suggested that the structure of intestinal microbiota of BG group at YPO stage was closer to HC group. LEfSe analysis suggested that BG_YPO stage enriched genus, such as Enterocloster and Hungatella_A_128155, which improved bile acid metabolism. Compared with BG_DPR stage, BG_YPO stage and HC group enriched Faecalibacterium and Roseburia, but depleted Streptococcus, while fecal SCFA concentrations increased. Conclusion: Patients with benign gallbladder disease and chronic cholecystitis after N-GPS treatment for over 1 year improved gut microbial community structure. With the improving bile acid metabolism, SCFA-producing bacteria increased and pathobionts decreased, which helped the intestinal microbiota structure of BG group at YPO stage restore and close to HC group. Trial Registration: Chinese Clinical Trial Registry identifier: ChiCTR1900028267.

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来源期刊
Gastroenterology Research and Practice
Gastroenterology Research and Practice GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
4.40
自引率
0.00%
发文量
91
审稿时长
1 months
期刊介绍: Gastroenterology Research and Practice is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on all areas of gastroenterology, hepatology, pancreas and biliary, and related cancers. The journal welcomes submissions on the physiology, pathophysiology, etiology, diagnosis and therapy of gastrointestinal diseases. The aim of the journal is to provide cutting edge research related to the field of gastroenterology, as well as digestive diseases and disorders. Topics of interest include: Management of pancreatic diseases Third space endoscopy Endoscopic resection Therapeutic endoscopy Therapeutic endosonography.
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