Thayná M. Avelino, Samarah V. Harb, Douglas Adamoski, Larissa C. M. Oliveira, Cintia D. S. Horinouchi, Rafael J. de Azevedo, Rafael A. Azoubel, Vanessa K. Thomaz, Fernanda A. H. Batista, Marcos Akira d’Ávila, Pedro L. Granja, Ana Carolina M. Figueira
{"title":"揭示真皮下层对基因表达的影响,推动生物打印全厚三维皮肤模型的发展。","authors":"Thayná M. Avelino, Samarah V. Harb, Douglas Adamoski, Larissa C. M. Oliveira, Cintia D. S. Horinouchi, Rafael J. de Azevedo, Rafael A. Azoubel, Vanessa K. Thomaz, Fernanda A. H. Batista, Marcos Akira d’Ávila, Pedro L. Granja, Ana Carolina M. Figueira","doi":"10.1038/s42003-024-07106-4","DOIUrl":null,"url":null,"abstract":"3D skin models have been explored as an alternative method to the use of animals in research and development. Usually, human skin equivalents comprise only epidermis or epidermis/dermis layers. Herein, we leverage 3D bioprinting technology to fabricate a full-thickness human skin equivalent with hypodermis (HSEH). The collagen hydrogel-based structure provides a mimetic environment for skin cells to adhere, proliferate and differentiate. The effective incorporation of the hypodermis layer is evidenced by scanning electron microscopy, immunofluorescence, and hematoxylin and eosin staining. The transcriptome results underscore the pivotal role of the hypodermis in orchestrating the genetic expression of a multitude of genes vital for skin functionality, including hydration, development and differentiation. Accordingly, we evidence the paramount significance of full-thickness human skin equivalents with hypodermis layer to provide an accurate in vitro platform for disease modeling and toxicology studies. We used 3D bioprinting to create a full-thickness human skin model with a hypodermis layer, offering an in vitro tool for disease and toxicology studies. This model replicates skin function and highlights the hypodermis’ role in tissue development.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":" ","pages":"1-13"},"PeriodicalIF":5.2000,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11555214/pdf/","citationCount":"0","resultStr":"{\"title\":\"Unveiling the impact of hypodermis on gene expression for advancing bioprinted full-thickness 3D skin models\",\"authors\":\"Thayná M. Avelino, Samarah V. Harb, Douglas Adamoski, Larissa C. M. Oliveira, Cintia D. S. Horinouchi, Rafael J. de Azevedo, Rafael A. Azoubel, Vanessa K. Thomaz, Fernanda A. H. Batista, Marcos Akira d’Ávila, Pedro L. Granja, Ana Carolina M. Figueira\",\"doi\":\"10.1038/s42003-024-07106-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"3D skin models have been explored as an alternative method to the use of animals in research and development. Usually, human skin equivalents comprise only epidermis or epidermis/dermis layers. Herein, we leverage 3D bioprinting technology to fabricate a full-thickness human skin equivalent with hypodermis (HSEH). The collagen hydrogel-based structure provides a mimetic environment for skin cells to adhere, proliferate and differentiate. The effective incorporation of the hypodermis layer is evidenced by scanning electron microscopy, immunofluorescence, and hematoxylin and eosin staining. The transcriptome results underscore the pivotal role of the hypodermis in orchestrating the genetic expression of a multitude of genes vital for skin functionality, including hydration, development and differentiation. Accordingly, we evidence the paramount significance of full-thickness human skin equivalents with hypodermis layer to provide an accurate in vitro platform for disease modeling and toxicology studies. We used 3D bioprinting to create a full-thickness human skin model with a hypodermis layer, offering an in vitro tool for disease and toxicology studies. This model replicates skin function and highlights the hypodermis’ role in tissue development.\",\"PeriodicalId\":10552,\"journal\":{\"name\":\"Communications Biology\",\"volume\":\" \",\"pages\":\"1-13\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2024-11-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11555214/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Communications Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.nature.com/articles/s42003-024-07106-4\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Communications Biology","FirstCategoryId":"99","ListUrlMain":"https://www.nature.com/articles/s42003-024-07106-4","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOLOGY","Score":null,"Total":0}
Unveiling the impact of hypodermis on gene expression for advancing bioprinted full-thickness 3D skin models
3D skin models have been explored as an alternative method to the use of animals in research and development. Usually, human skin equivalents comprise only epidermis or epidermis/dermis layers. Herein, we leverage 3D bioprinting technology to fabricate a full-thickness human skin equivalent with hypodermis (HSEH). The collagen hydrogel-based structure provides a mimetic environment for skin cells to adhere, proliferate and differentiate. The effective incorporation of the hypodermis layer is evidenced by scanning electron microscopy, immunofluorescence, and hematoxylin and eosin staining. The transcriptome results underscore the pivotal role of the hypodermis in orchestrating the genetic expression of a multitude of genes vital for skin functionality, including hydration, development and differentiation. Accordingly, we evidence the paramount significance of full-thickness human skin equivalents with hypodermis layer to provide an accurate in vitro platform for disease modeling and toxicology studies. We used 3D bioprinting to create a full-thickness human skin model with a hypodermis layer, offering an in vitro tool for disease and toxicology studies. This model replicates skin function and highlights the hypodermis’ role in tissue development.
期刊介绍:
Communications Biology is an open access journal from Nature Research publishing high-quality research, reviews and commentary in all areas of the biological sciences. Research papers published by the journal represent significant advances bringing new biological insight to a specialized area of research.