Study on Extraction and Bioactivity of Polysaccharides from Viburnum sargentii Koehne with Deep Eutectic Solvent.

The fruits of Viburnum sargentii Koehne (Vsk) are rich in bioactive polysaccharides and polyphenols, which exhibit anti-inflammatory, antioxidant, and hypoglycemic properties. This study explored the use of deep eutectic solvents (DES), specifically urea-choline chloride, as a green extraction medium to maximize polysaccharide yield from Vsk polysaccharides. Extraction conditions, including solvent composition, temperature, and time, were optimized using response surface methodology, achieving significantly higher yields and purity compared to hot water extraction. The polysaccharides extracted through DES showed a more homogeneous molecular structure, with reduced protein and pigment impurities. Bioactivity assays confirmed potent antioxidant and hypoglycemic effects, with DES-extracted polysaccharides displaying enhanced reducing power, free radical scavenging capabilities, and notable α-amylase inhibition compared to those obtained by conventional methods. These findings highlight DES extraction as an effective approach not only to improve yield and purity but also to enhance the bioactivity of extracted compounds. Consequently, DES-extracted Vsk polysaccharides show potential for use in developing functional foods and nutraceuticals. This study advances sustainable extraction technologies, positioning Vsk polysaccharides as valuable bioactive compounds with promising health applications.