小鼠骨骼肌再生和衰老的免疫环境。

IF 7.5 1区 生物学 Q1 CELL BIOLOGY
Neuza S Sousa, Marta Bica, Margarida F Brás, Ana C Sousa, Inês B Antunes, Isabel A Encarnação, Tiago M Costa, Inês B Martins, Nuno L Barbosa-Morais, Pedro Sousa-Victor, Joana Neves
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引用次数: 0

摘要

与年龄相关的免疫系统改变开始成为导致老化器官受损的关键因素。再生能力下降是组织衰老的标志;然而,人们刚刚开始探索免疫衰老对再生失败的影响。在这里,我们采用了一种将单细胞 RNA 测序与流式细胞术、组织学分析和功能测定相结合的策略,以单细胞分辨率对损伤后时间进程中的衰老再生骨骼肌的免疫环境进行了全面分析。我们的研究结果表明,骨骼肌内的免疫群体具有意想不到的复杂性和功能异质性,而这些免疫群体一直被认为是同质的。此外,我们还发现髓系和淋巴系在衰老过程中都发生了深刻的重塑。这些发现挑战了关于骨骼肌再生的免疫调节的既有观念,为改善骨骼肌健康和衰老过程中的再生能力提供了一系列潜在靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The immune landscape of murine skeletal muscle regeneration and aging.

Age-related alterations in the immune system are starting to emerge as key contributors to impairments found in aged organs. A decline in regenerative capacity is a hallmark of tissue aging; however, the contribution of immune aging to regenerative failure is just starting to be explored. Here, we apply a strategy combining single-cell RNA sequencing with flow cytometry, histological analysis, and functional assays to perform a complete analysis of the immune environment of the aged regenerating skeletal muscle on a time course following injury with single-cell resolution. Our results reveal an unanticipated complexity and functional heterogeneity in immune populations within the skeletal muscle that have been regarded as homogeneous. Furthermore, we uncover a profound remodeling of both myeloid and lymphoid compartments in aging. These discoveries challenge established notions on immune regulation of skeletal muscle regeneration, providing a set of potential targets to improve skeletal muscle health and regenerative capacity in aging.

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来源期刊
Cell reports
Cell reports CELL BIOLOGY-
CiteScore
13.80
自引率
1.10%
发文量
1305
审稿时长
77 days
期刊介绍: Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.
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