C F D S Andrade, M N Souza, I I D S Dantas, A S K Fonseca, N Ikuta, D Kipper, V R Lunge
{"title":"用快速等温法对家禽样本中的肠炎沙门氏菌和海德堡血清型进行特异性分子检测。","authors":"C F D S Andrade, M N Souza, I I D S Dantas, A S K Fonseca, N Ikuta, D Kipper, V R Lunge","doi":"10.1080/00071668.2024.2419623","DOIUrl":null,"url":null,"abstract":"<p><p>1. Loop-mediated isothermal amplification (LAMP) assays were developed to detect <i>Salmonella enterica</i> subspecies <i>enterica</i> serotypes Enteritidis and Heidelberg in poultry farms. These serotype-specific methods were evaluated in comparison with PCR in the analysis of different <i>Salmonella</i> spp. serotypes from a culture collection and poultry farm samples.2. The results demonstrated the specific amplification of the genetic targets <i>saf</i>A in all <i>S</i>. Enteritidis (<i>n</i> = 10) and <i>ACF69659</i> in all <i>S</i>. Heidelberg (<i>n</i> = 36) isolates from the culture collection. The remaining isolates from other <i>Salmonella</i> spp. serotypes (<i>n</i> = 84) and bacterial species (<i>n</i> = 8) were negative in both LAMP assays.3. The methods detected DNAs from <i>S</i>. Enteritidis and <i>S</i>. Heidelberg after a single-step pre-enrichment in buffered peptone water of the poultry samples, which agreed with previously developed PCR methods to detect these same two serotypes.4. In conclusion, LAMP assays were useful for rapid serotype-specific detection, being suitable for surveillance purposes in resource-limited environments such as poultry farms.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-7"},"PeriodicalIF":1.6000,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Salmonella enterica</i> Enteritidis and Heidelberg serotype-specific molecular detection in poultry samples by a rapid isothermal method.\",\"authors\":\"C F D S Andrade, M N Souza, I I D S Dantas, A S K Fonseca, N Ikuta, D Kipper, V R Lunge\",\"doi\":\"10.1080/00071668.2024.2419623\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>1. Loop-mediated isothermal amplification (LAMP) assays were developed to detect <i>Salmonella enterica</i> subspecies <i>enterica</i> serotypes Enteritidis and Heidelberg in poultry farms. These serotype-specific methods were evaluated in comparison with PCR in the analysis of different <i>Salmonella</i> spp. serotypes from a culture collection and poultry farm samples.2. The results demonstrated the specific amplification of the genetic targets <i>saf</i>A in all <i>S</i>. Enteritidis (<i>n</i> = 10) and <i>ACF69659</i> in all <i>S</i>. Heidelberg (<i>n</i> = 36) isolates from the culture collection. The remaining isolates from other <i>Salmonella</i> spp. serotypes (<i>n</i> = 84) and bacterial species (<i>n</i> = 8) were negative in both LAMP assays.3. The methods detected DNAs from <i>S</i>. Enteritidis and <i>S</i>. Heidelberg after a single-step pre-enrichment in buffered peptone water of the poultry samples, which agreed with previously developed PCR methods to detect these same two serotypes.4. In conclusion, LAMP assays were useful for rapid serotype-specific detection, being suitable for surveillance purposes in resource-limited environments such as poultry farms.</p>\",\"PeriodicalId\":9322,\"journal\":{\"name\":\"British Poultry Science\",\"volume\":\" \",\"pages\":\"1-7\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-11-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"British Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/00071668.2024.2419623\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"British Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/00071668.2024.2419623","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Salmonella enterica Enteritidis and Heidelberg serotype-specific molecular detection in poultry samples by a rapid isothermal method.
1. Loop-mediated isothermal amplification (LAMP) assays were developed to detect Salmonella enterica subspecies enterica serotypes Enteritidis and Heidelberg in poultry farms. These serotype-specific methods were evaluated in comparison with PCR in the analysis of different Salmonella spp. serotypes from a culture collection and poultry farm samples.2. The results demonstrated the specific amplification of the genetic targets safA in all S. Enteritidis (n = 10) and ACF69659 in all S. Heidelberg (n = 36) isolates from the culture collection. The remaining isolates from other Salmonella spp. serotypes (n = 84) and bacterial species (n = 8) were negative in both LAMP assays.3. The methods detected DNAs from S. Enteritidis and S. Heidelberg after a single-step pre-enrichment in buffered peptone water of the poultry samples, which agreed with previously developed PCR methods to detect these same two serotypes.4. In conclusion, LAMP assays were useful for rapid serotype-specific detection, being suitable for surveillance purposes in resource-limited environments such as poultry farms.
期刊介绍：
From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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