通过长期实时成像揭示果蝇造血的新特征。

IF 2.5 3区 生物学 Q2 DEVELOPMENTAL BIOLOGY
Kevin Y.L. Ho , Annie Y.J. Ou , Nicholas Samuelson , Guy Tanentzapf
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引用次数: 0

摘要

干细胞受到持续调控,以确保干细胞分化和自我更新之间保持正确平衡。干细胞调控的动态性和持续性,以及干细胞通常所处的复杂信号微环境,意味着在内源性环境中实时研究干细胞比静态固定样本方法具有多种优势。我们最近描述了一种对果蝇幼虫造血器官淋巴腺(LG)中的血液祖细胞进行长期、体外、活体成像的方法。这种方法使我们能够实时分析果蝇造血的多个方面,这是以前无法实现的。在这里,我们描述了从我们的定量实时成像方法中获得的新见解。这些见解包括在祖细胞中发现大量丝状体,并描述其形态和动态;通过同时追踪数千个含有内化多莫受体的囊泡,对祖细胞中的 JAK/STAT 信号进行可视化和定量分析;定量分析血液祖细胞中线粒体的位置、形态和动态;长期跟踪 LG 中成熟血细胞的细胞分裂和迁移模式;长期跟踪远端承诺祖细胞的多种细胞行为;分析 LG 次级叶中血液祖细胞的 Ca2+ 信号传导。这些观察结果共同说明了对蝇造血过程进行实时成像的能力,并确定了 LG 中许多以前未曾描述过的过程和行为,这些过程和行为可能在祖细胞分化和自我更新的调控中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Novel features of Drosophila hematopoiesis uncovered by long-term live imaging

Novel features of Drosophila hematopoiesis uncovered by long-term live imaging
Stem cells are subject to continuous regulation to ensure that the correct balance between stem cell differentiation and self-renewal is maintained. The dynamic and ongoing nature of stem cell regulation, as well as the complex signaling microenvironment in which stem cells are typically found, means that studying them in their endogenous environment in real time has multiple advantages over static fixed-sample approaches. We recently described a method for long-term, ex-vivo, live imaging of the blood progenitors in the Drosophila larval hematopoietic organ, the Lymph Gland (LG). This methodology has allowed us to analyze multiple aspects of fly hematopoiesis, in real time, in a manner that could not be carried out previously. Here, we describe novel insights derived from our quantitative live imaging approach. These insights include: the identification of extensive filopodia in the progenitors and description of their morphology and dynamics; visualization and quantitative analysis of JAK/STAT signaling in progenitors by the simultaneous tracking of thousands of vesicles containing internalized Domeless receptors; quantitative analysis of the location, morphology, and dynamics of mitochondria in blood progenitors; long-term tracking of patterns of cell division and migration of mature blood cell in the LG; long-term tracking of multiple cell behaviors in the distal committed progenitors; analysis of Ca2+ signaling of blood progenitors in the secondary lobes of the LG. Together, these observations illustrate the power of imaging fly hematopoiesis in real time and identify many previously undescribed processes and behaviors in the LG that are likely to play important roles in the regulation of progenitor differentiation and self-renewal.
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来源期刊
Developmental biology
Developmental biology 生物-发育生物学
CiteScore
5.30
自引率
3.70%
发文量
182
审稿时长
1.5 months
期刊介绍: Developmental Biology (DB) publishes original research on mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, genetic and evolutionary levels. Areas of particular emphasis include transcriptional control mechanisms, embryonic patterning, cell-cell interactions, growth factors and signal transduction, and regulatory hierarchies in developing plants and animals.
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