啮齿动物血浆中巴氯芬定量的新型分析方法。

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-01-01 Epub Date: 2024-11-13 DOI:10.1002/bmc.6038

Bindu Dhiman, Mithlesh Yadav, Anju Dhiman, Saurabh Satija

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引用次数: 0

摘要

建立了一种简便、准确、稳健的反相高效液相色谱（RP-HPLC）方法，用于定量检测大鼠血浆中的巴氯芬。该方法在 10-50 μg/mL 浓度范围内线性关系良好（r2 = 0.9936）。根据 ICH 指南评估了该方法的精密度、准确度、检出限（LOD）、定量限（LOQ）和稳健性。检测限和定量限分别为 0.076197 和 0.23090 μg/mL。该方法为血浆中巴氯芬的定量提供了一种有效的方法，适用于药代动力学和生物利用度研究。这项研究的新颖之处在于优化了其在实验室中的常规使用，确保了在不同条件下和不同分析人员之间的最小变异的重现性。

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Novel analytical approach for baclofen quantification in rodent plasma.

A simple, accurate, and robust reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the quantification of Baclofen in rat plasma. The method demonstrated high degree of linearity (r² = 0.9936) across a concentration range of 10-50 μg/mL. Precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and robustness were evaluated according to ICH guidelines. The LOD and LOQ were found to be 0.076197 and 0.23090 μg/mL, respectively. This method provides an efficient approach for Baclofen quantification in plasma, making it suitable for pharmacokinetic and bioavailability studies. The novelty of this study lies in its optimization for routine use in laboratories, ensuring reproducibility with minimal variations across different conditions and analysts.

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.