22 和 50 kHz 大鼠超声波发声重放显示了行为和与情感处理相关的脑区 cFos 的性别差异。

IF 2.6 3区心理学 Q2 BEHAVIORAL SCIENCES

Behavioural Brain Research Pub Date : 2024-11-08 DOI:10.1016/j.bbr.2024.115326

Sydney M. Bonauto, Kaya A. Patel, Jennifer A. Honeycutt

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引用次数: 0

摘要

成年大鼠使用超声波发声（USV）频率进行交流，表示消极（22 kHz）或积极（50 kHz）的情感状态。回放 USV 可作为一种伦理学转化方法，用于研究对社会交流状态做出反应的情感处理。然而，很少有研究同时对雄性和雌性大鼠的行为和神经效应进行研究。在这里，成年雄性和雌性 Sprague-Dawley 大鼠经历了 20 分钟的开阔地测试（OFT），测试过程中会听到安静、22 kHz 或 50 kHz 的 USV 回放录音。对中心探索和运动活动进行了分析，以确定重放效应的性别差异。结果表明，与雄性相比，雌性在该范例中对USV重放的频率特异性效应表现出更高的敏感性。50 kHz USV回放会立即增加雌性的中心探索和运动活动，这表明50 kHz USV具有食欲性。与 50 千赫的USV相比，22 千赫的USV播放最初会抑制雌性在OFT中的中心探索。然而，在长时间播放后，雌性对 22 kHz 的行为策略有所改变。OFT后，通过即时早期基因cFos对神经活动进行了量化。cFos 定量结果显示，在与情感处理相关的大脑区域，包括前额叶皮层、杏仁核、纹状体末端床核和凹凸核，神经招募存在性别和区域特异性差异。总之，这项研究为了解性别如何影响对 USV 重放的行为和神经反应提供了一个标准基线，可以利用它来研究焦虑、交流和与伦理相关的情感。

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22 and 50 kHz rat ultrasonic vocalization playback reveals sex differences in behavior and cFos in brain regions associated with affective processing

Adult rats communicate using ultrasonic vocalization (USV) frequencies indicating negative (22 kHz) or positive (50 kHz) affective states. Playback of USVs can serve as an ethologically translational method to study affective processing in response to socially communicated states. However, few studies have examined behavioral and neural effects of USV playback in both male and female rats. Here, adult male and female Sprague-Dawley rats experienced a 20-min open field test (OFT) with either silence, 22 kHz, or 50 kHz recorded USV playback. Center exploration and locomotor activity were analyzed to characterize sex differences in playback effects. Results suggest that females display greater sensitivity to frequency-specific effects of USV playback in this paradigm compared to males. 50 kHz USV playback evoked an immediate increase in center exploration and locomotor activity in females, indicating the appetitive nature of 50 kHz USVs. Initially, 22 kHz playback inhibited center exploration in the OFT compared to 50 kHz. However, females exhibited a switch in behavioral strategy in response to 22 kHz following prolonged playback. Following OFT, neural activity was quantified via the immediate early gene cFos. Results from cFos quantification showed sex- and region-specific differences in neural recruitment in areas of the brain associated with affective processing, including the prefrontal cortex, amygdala, bed nucleus of the stria terminalis, and nucleus accumbens. Taken together, this work provides a normative baseline for understanding how sex influences behavioral and neural responses to USV playback, which can be leveraged to study anxiety, communication, and affect in an ethologically relevant assay.

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来源期刊

Behavioural Brain Research 医学-行为科学

CiteScore

5.60

自引率

0.00%

发文量

383

审稿时长

61 days

期刊介绍： Behavioural Brain Research is an international, interdisciplinary journal dedicated to the publication of articles in the field of behavioural neuroscience, broadly defined. Contributions from the entire range of disciplines that comprise the neurosciences, behavioural sciences or cognitive sciences are appropriate, as long as the goal is to delineate the neural mechanisms underlying behaviour. Thus, studies may range from neurophysiological, neuroanatomical, neurochemical or neuropharmacological analysis of brain-behaviour relations, including the use of molecular genetic or behavioural genetic approaches, to studies that involve the use of brain imaging techniques, to neuroethological studies. Reports of original research, of major methodological advances, or of novel conceptual approaches are all encouraged. The journal will also consider critical reviews on selected topics.