Qianwen Zhang, Yue Liu, Chunli Zhang, Dawei Xu, Ana L. Medina-Fraga, Baoguo Wu, Chenyang Guo, MeLongying Wangzha, Guoqian Yang, Danmeng Zhu, David Weiss, Carlos L. Ballaré, Li Lin, Ruohe Yin
{"title":"SlSPA3调节番茄中SlUVR8的核丰度。","authors":"Qianwen Zhang, Yue Liu, Chunli Zhang, Dawei Xu, Ana L. Medina-Fraga, Baoguo Wu, Chenyang Guo, MeLongying Wangzha, Guoqian Yang, Danmeng Zhu, David Weiss, Carlos L. Ballaré, Li Lin, Ruohe Yin","doi":"10.1111/tpj.17135","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Tomato (<i>Solanum lycopersicum</i> L.) is an important model plant species in photomorphogenesis research. Ultraviolet B (UV-B) induces the dissociation of homodimers of the photoreceptor UV RESISTANCE LOCUS8 (UVR8) into monomers, which translocate into the nucleus. Nuclear accumulation of UVR8 is a prerequisite for its signaling function. Previous studies have reported that SUPPRESSOR OF PHYTOCHROME A-105 (SPA) family members may regulate UV-B signaling in Arabidopsis (<i>Arabidopsis thaliana</i>); however, the underlying mechanism is unknown. Here, we show that the tomato genome encodes four SPA (SlSPA) orthologs. Genome-edited <i>Slspa3</i> mutants exhibited enhanced photomorphogenic responses in white light, suggesting that SlSPA3 inhibits general photomorphogenesis. By contrast, UVR8-mediated gene expression in response to UV-B was compromised in <i>Slspa3</i> mutants, suggesting that SlSPA3 promotes UV-B signaling. UV-B-induced nuclear accumulation of UVR8, which is essential for UV-B signaling, was reduced in the <i>Slspa3</i> mutants. Moreover, UV-B-induced nuclear accumulation of UVR8 was also reduced in the Arabidopsis <i>spa1 spa2 spa3</i> and <i>spa1 spa2 spa4</i> triple mutants, indicating a conserved mechanism in these two species. Notably, <i>spa1 spa2 spa4</i> exhibited normal UV-B-induced interaction between UVR8 and the plant morphogenesis repressor CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1). This suggests that the well-established mechanisms of UVR8 nuclear retention remained unaffected in <i>spa1 spa2 spa4</i>. Thus, our work uncovered a potentially unrecognized mechanism by which SPA proteins regulate UV-B signaling through the promotion of UVR8 nuclear abundance in land plants.</p>\n </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2656-2667"},"PeriodicalIF":6.2000,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato\",\"authors\":\"Qianwen Zhang, Yue Liu, Chunli Zhang, Dawei Xu, Ana L. Medina-Fraga, Baoguo Wu, Chenyang Guo, MeLongying Wangzha, Guoqian Yang, Danmeng Zhu, David Weiss, Carlos L. Ballaré, Li Lin, Ruohe Yin\",\"doi\":\"10.1111/tpj.17135\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Tomato (<i>Solanum lycopersicum</i> L.) is an important model plant species in photomorphogenesis research. Ultraviolet B (UV-B) induces the dissociation of homodimers of the photoreceptor UV RESISTANCE LOCUS8 (UVR8) into monomers, which translocate into the nucleus. Nuclear accumulation of UVR8 is a prerequisite for its signaling function. Previous studies have reported that SUPPRESSOR OF PHYTOCHROME A-105 (SPA) family members may regulate UV-B signaling in Arabidopsis (<i>Arabidopsis thaliana</i>); however, the underlying mechanism is unknown. Here, we show that the tomato genome encodes four SPA (SlSPA) orthologs. Genome-edited <i>Slspa3</i> mutants exhibited enhanced photomorphogenic responses in white light, suggesting that SlSPA3 inhibits general photomorphogenesis. By contrast, UVR8-mediated gene expression in response to UV-B was compromised in <i>Slspa3</i> mutants, suggesting that SlSPA3 promotes UV-B signaling. UV-B-induced nuclear accumulation of UVR8, which is essential for UV-B signaling, was reduced in the <i>Slspa3</i> mutants. Moreover, UV-B-induced nuclear accumulation of UVR8 was also reduced in the Arabidopsis <i>spa1 spa2 spa3</i> and <i>spa1 spa2 spa4</i> triple mutants, indicating a conserved mechanism in these two species. Notably, <i>spa1 spa2 spa4</i> exhibited normal UV-B-induced interaction between UVR8 and the plant morphogenesis repressor CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1). This suggests that the well-established mechanisms of UVR8 nuclear retention remained unaffected in <i>spa1 spa2 spa4</i>. Thus, our work uncovered a potentially unrecognized mechanism by which SPA proteins regulate UV-B signaling through the promotion of UVR8 nuclear abundance in land plants.</p>\\n </div>\",\"PeriodicalId\":233,\"journal\":{\"name\":\"The Plant Journal\",\"volume\":\"120 6\",\"pages\":\"2656-2667\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2024-11-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Plant Journal\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/tpj.17135\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tpj.17135","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato
Tomato (Solanum lycopersicum L.) is an important model plant species in photomorphogenesis research. Ultraviolet B (UV-B) induces the dissociation of homodimers of the photoreceptor UV RESISTANCE LOCUS8 (UVR8) into monomers, which translocate into the nucleus. Nuclear accumulation of UVR8 is a prerequisite for its signaling function. Previous studies have reported that SUPPRESSOR OF PHYTOCHROME A-105 (SPA) family members may regulate UV-B signaling in Arabidopsis (Arabidopsis thaliana); however, the underlying mechanism is unknown. Here, we show that the tomato genome encodes four SPA (SlSPA) orthologs. Genome-edited Slspa3 mutants exhibited enhanced photomorphogenic responses in white light, suggesting that SlSPA3 inhibits general photomorphogenesis. By contrast, UVR8-mediated gene expression in response to UV-B was compromised in Slspa3 mutants, suggesting that SlSPA3 promotes UV-B signaling. UV-B-induced nuclear accumulation of UVR8, which is essential for UV-B signaling, was reduced in the Slspa3 mutants. Moreover, UV-B-induced nuclear accumulation of UVR8 was also reduced in the Arabidopsis spa1 spa2 spa3 and spa1 spa2 spa4 triple mutants, indicating a conserved mechanism in these two species. Notably, spa1 spa2 spa4 exhibited normal UV-B-induced interaction between UVR8 and the plant morphogenesis repressor CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1). This suggests that the well-established mechanisms of UVR8 nuclear retention remained unaffected in spa1 spa2 spa4. Thus, our work uncovered a potentially unrecognized mechanism by which SPA proteins regulate UV-B signaling through the promotion of UVR8 nuclear abundance in land plants.
期刊介绍:
Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community.
Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.