编码蒽酸合成酶 α-亚基的水稻早期叶裂和感光 1(ELS1)发生突变,通过激活水稻色氨酸合成途径诱导 ROS 积累和细胞死亡。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
Wenhao Li, Weimin Cheng, Hongrui Jiang, Cheng Fang, Lingling Peng, Liangzhi Tao, Yue Zhan, Xianzhong Huang, Bojun Ma, Xifeng Chen, Yuejin Wu, Binmei Liu, Xiangdong Fu, Kun Wu, Yafeng Ye
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引用次数: 0

摘要

病变模拟突变体(LMMs)是揭示植物细胞程序性死亡(PCD)分子机制的宝贵资源。尽管研究范围广泛,但各种 LMM 中 PCD 和病变形成的调控机制仍有待全面阐明。在本研究中,我们发现了一种名为早期叶片病变和衰老 1(els1)的水稻 LMM,通过基于图谱的克隆克隆了其致病基因,并通过互补证实了其功能。ELS1 编码参与花青素生物合成的花青素合成酶 α-亚基。它主要定位于叶绿体,并主要在受光组织中表达。ELS1 基因突变会通过遗传补偿反应引发其同源基因 ASA1 的上调,从而激活色氨酸(Trp)合成途径和氨基酸代谢。异常 Trp 衍生的中间代谢产物的积累导致活性氧(ROS)产生,els1 突变体的 PCD 异常,最终造成叶片病变表型。els1突变体还表现出叶绿素含量降低、叶绿体降解和叶片衰老相关基因上调以及光合蛋白活性降低,表明 ELS1 在叶绿体发育过程中发挥作用。这些因素共同导致了在els1突变体中观察到的叶片过早衰老。我们的研究结果揭示了 ELS1 在调节水稻 ROS 积累和 PCD 中的作用,为研究叶片病变和衰老的分子机制提供了进一步的遗传学见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mutation of rice EARLY LEAF LESION AND SENESCENCE 1 (ELS1), which encodes an anthranilate synthase α-subunit, induces ROS accumulation and cell death through activating the tryptophan synthesis pathway in rice

Lesion-mimic mutants (LMMs) serve as valuable resources for uncovering the molecular mechanisms that govern programmed cell death (PCD) in plants. Despite extensive research, the regulatory mechanisms of PCD and lesion formation in various LMMs remain to be fully elucidated. In this study, we identified a rice LMM named early leaf lesion and senescence 1 (els1), cloned the causal gene through map-based cloning, and confirmed its function through complementation. ELS1 encodes an anthranilate synthase α-subunit involved in anthranilate biosynthesis. It is predominantly localized in chloroplasts and is primarily expressed in light-exposed tissues. Mutation of ELS1 triggers upregulation of its homologous gene, ASA1, via a genetic compensation response, leading to the activation of the tryptophan (Trp) synthesis pathway and amino acid metabolism. The accumulation of abnormal Trp-derived intermediate metabolites results in reactive oxygen species (ROS) production and abnormal PCD in the els1 mutant, ultimately causing the leaf lesion phenotype. The els1 mutant also exhibits reduced chlorophyll content, upregulation of genes related to chloroplast degradation and leaf senescence, and decreased activity of photosynthetic proteins, indicating that ELS1 plays a role in chloroplast development. These factors collectively contribute to the premature leaf senescence observed in the els1 mutant. Our findings shed light on the role of ELS1 in regulating ROS accumulation and PCD in rice, providing further genetic insights into the molecular mechanisms governing leaf lesions and senescence.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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