防御相关胼胝质合成酶 PMR4 促进拟南芥根毛胼胝质沉积和对磷酸盐缺乏的适应。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
Kentaro Okada, Koei Yachi, Tan Anh Nhi Nguyen, Satomi Kanno, Shigetaka Yasuda, Haruna Tadai, Chika Tateda, Tae-Hong Lee, Uyen Nguyen, Kanako Inoue, Natsuki Tsuchida, Taiga Ishihara, Shunsuke Miyashima, Kei Hiruma, Kyoko Miwa, Takaki Maekawa, Michitaka Notaguchi, Yusuke Saijo
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引用次数: 0

摘要

植物主要以无机磷酸盐(Pi)的形式从土壤中获取磷(P)。在缺磷情况下,植物会产生一系列生理和形态反应,即磷酸盐饥饿反应(PSR),从而提高磷的获取和利用效率。然而,植物适应π缺乏的机制仍有待阐明。在此,我们报告了拟南芥根毛在 Pi 缺乏条件下被诱导沉积一种称为 Callose 的 β-1,3-葡聚糖聚合物,其沉积方式与 PSR 调节 PHR1/PHL1 转录因子和 LPR1/LPR2 铁氧化酶无关。遗传研究发现,PMR4(GSL5)胼胝质合成酶是π缺乏根毛中胼胝质沉积所必需的。PMR4 的缺失也会降低芽中 Pi 的获取以及低 Pi 条件下的植物生长。同时破坏 SID2(介导与防御相关的水杨酸(SA)生物合成)也不会恢复这些缺陷,这就排除了导致观察到的 pmr4 表型的 SA 防御激活的原因。根毛细胞中特异表达 PMR4 的植株的嫁接实验和特征描述表明,细胞类型中的 PMR4 池有助于π缺乏下的芽生长。因此,我们的研究结果表明 PMR4 在植物适应π缺乏的过程中发挥着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Defense-related callose synthase PMR4 promotes root hair callose deposition and adaptation to phosphate deficiency in Arabidopsis thaliana

Defense-related callose synthase PMR4 promotes root hair callose deposition and adaptation to phosphate deficiency in Arabidopsis thaliana

Plants acquire phosphorus (P) primarily as inorganic phosphate (Pi) from the soil. Under Pi deficiency, plants induce an array of physiological and morphological responses, termed phosphate starvation response (PSR), thereby increasing Pi acquisition and use efficiency. However, the mechanisms by which plants adapt to Pi deficiency remain to be elucidated. Here, we report that deposition of a β-1,3-glucan polymer called callose is induced in Arabidopsis thaliana root hairs under Pi deficiency, in a manner independent of PSR-regulating PHR1/PHL1 transcription factors and LPR1/LPR2 ferroxidases. Genetic studies revealed PMR4 (GSL5) callose synthase being required for the callose deposition in Pi-depleted root hairs. Loss of PMR4 also reduces Pi acquisition in shoots and plant growth under low Pi conditions. The defects are not recovered by simultaneous disruption of SID2, mediating defense-associated salicylic acid (SA) biosynthesis, excluding SA defense activation from the cause of the observed pmr4 phenotypes. Grafting experiments and characterization of plants expressing PMR4 specifically in root hair cells suggest that a PMR4 pool in the cell type contributes to shoot growth under Pi deficiency. Our findings thus suggest an important role for PMR4 in plant adaptation to Pi deficiency.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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