PtobZIP55-PtoMYB170模块调节杨树在适应低氮可用性过程中的木材解剖和化学特性。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jiangting Wu, Shurong Deng, Yang Wang, Chenlin Jia, Jia Wei, Mengyan Zhou, Dongyue Zhu, Zhuorong Li, Payam Fayyaz, Zhi-Bin Luo, Jing Zhou, Wenguang Shi
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引用次数: 0

摘要

杨树种植园通常建在贫氮土地上,杨树的生长和木材形成受到低氮(LN)可用性的限制。然而,特定基因在适应低氮可用性过程中调控木材形成的分子机制仍不清楚。在这里,我们报告了一个以前未被发现的模块,即基本区/亮氨酸拉链 55(PtobZIP55)-PtoMYB170,它能调节杨树在适应低氮可用性时的木材形成。PtobZIP55 在杨木中高表达,并由 LN 诱导。在过表达 PtobZIP55 的杨树中发现了木材解剖性质的改变和木质化的增加,而在 PtobZIP55 基因敲除的杨树中则发现了相反的结果。分子和转基因分析表明,PtobZIP55 直接与 PtoMYB170 的启动子序列结合,激活其转录。在 LN 条件下,PtoMYB170 转基因杨树的表型与 PtobZIP55 转基因杨树相似。进一步的分子分析表明,PtoMYB170 直接结合了木质素生物合成基因的启动子序列,激活了这些基因的转录,从而增加了 LN 处理的杨木中木质素的浓度。这些结果表明,PtobZIP55 能激活 PtoMYB170 的转录,而 PtoMYB170 又能正向调节木质素生物合成基因,从而在适应 LN 的情况下增加托木杨木材中的木质素沉积。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The PtobZIP55-PtoMYB170 module regulates the wood anatomical and chemical properties of Populus tomentosa in acclimation to low nitrogen availability.

Poplar plantations are often established on nitrogen-poor land, and poplar growth and wood formation are constrained by low nitrogen (LN) availability. However, the molecular mechanisms by which specific genes regulate wood formation in acclimation to LN availability remain unclear. Here, we report a previously unrecognized module, basic region/leucine zipper 55 (PtobZIP55)-PtoMYB170, which regulates the wood formation of Populus tomentosa in acclimation to LN availability. PtobZIP55 was highly expressed in poplar wood and induced by LN. Altered wood anatomical properties and increased lignification were detected in PtobZIP55-overexpressing poplars, whereas the opposite results were detected in PtobZIP55-knockout poplars. Molecular and transgenic analyses revealed that PtobZIP55 directly binds to the promoter sequence of PtoMYB170 to activate its transcription. The phenotypes of PtoMYB170 transgenic poplars were similar to those of PtobZIP55 transgenic poplars under LN conditions. Further molecular analyses revealed that PtoMYB170 directly bound the promoter sequences of lignin biosynthetic genes to activate their transcription to increase lignin concentrations in LN-treated poplar wood. These results suggest that PtobZIP55 activates PtoMYB170 transcription, which in turn positively regulates lignin biosynthetic genes, increasing lignin deposition in the wood of P. tomentosa in the context of acclimation to LN availability.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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