Nancy Ferrentino, Taha Behroozi Kohlan, Shokoufeh Mehrtashfar, Anna Finne-Wistrand, Daniela Pappalardo
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引用次数: 0
摘要
刺激响应型聚合物纳米粒子(NPs)可作为智能给药系统(DDSs),在外部或内部刺激下触发药物释放。本研究合成了一种由三嵌段聚(ε-己内酯)-聚(乙二醇)-聚(ε-己内酯)(PCL-SS-PEG-SS-PCL)共聚物组成的双响应 DDS,PCL 和 PEG 之间存在二硫键。该共聚物被香豆素官能化,对近红外(NIR)光照射敏感,S-S 键可被 GSH(10 mM)裂解。通过核磁共振、尺寸排阻色谱和傅立叶变换红外分析对其进行了表征。通过共聚物在水中的自组装制备了负载尼罗红(NR)的 NPs,并通过动态光散射和场发射扫描电子显微镜进行了分析。荧光光谱法监测了紫外线/近红外光照射以及 GSH 浓度对 NR 释放的影响,而同时照射紫外线/近红外光和细胞内 GSH 浓度会导致 NR 释放更快。AlamarBlue 分析表明负载 NR 的 NPs 具有令人满意的细胞活力,而荧光显微镜和荧光发射测量则研究了 NPs 在人真皮成纤维细胞中的细胞吸收情况。
Dual-Responsive Nanoparticles for Smart Drug Delivery: A NIR Light-Sensitive and Redox-Reactive PEG-PCL-Based System.
Stimuli-responsive polymeric nanoparticles (NPs) can serve as smart drug delivery systems (DDSs) by triggering drug release upon external or internal stimuli. A dual-responsive DDS made of a triblock poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-SS-PEG-SS-PCL) copolymer, bearing disulfide bonds between PCL and PEG, was synthesized. The copolymer was functionalized with coumarin and sensitive to near-infrared (NIR) light irradiation, while the S-S bonds could be cleaved by GSH (10 mM). Characterization was achieved by nuclear magnetic resonance, size exclusion chromatography, and Fourier transform infrared analyses. Nile Red (NR)-loaded NPs were prepared through self-assembly of the copolymer in water and analyzed by dynamic light scattering and field-emission scanning electron microscopy. The NR release upon ultraviolet (UV)/NIR light irradiation as well as by GSH concentrations was monitored by using fluorescence spectroscopy, while simultaneous exposure to UV/NIR light and intracellular GSH concentration led to faster NR release. AlamarBlue assay showed satisfactory cell viability of the NR-loaded NPs, while their cellular uptake in human dermal fibroblast cells was investigated by fluorescence microscopy and fluorescence emission measurements.
期刊介绍:
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