组织安全的低温等离子处理技术,用于有效处理钛表面成熟的种植体周围炎生物膜。

IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS
Beatriz H D Panariello, Giovanna C Denucci, Caroline C Tonon, George J Eckert, Lukasz Witek, Vasudev V Nayak, Paulo G Coelho, Simone Duarte
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引用次数: 0

摘要

种植体独特的螺纹设计和微观结构给机械清创去除生物膜带来了挑战。与单个浮游细胞相比,生物膜对抗菌剂的耐药性更强,因此在治疗种植体周围炎的牙周治疗过程中需要有效清除生物膜。为了解决这个问题,我们的团队评估了低温等离子体(LTP)对被与种植体周围炎相关的多菌种生物膜污染的钛盘进行消毒的效果,特别关注成熟 14 天和 21 天的生物膜,以及在 StraumannⓇ Ti-SLA 种植体上形成 21 天的生物膜。这些生物膜包括在厌氧条件下生长的奈氏放线菌、牙龈卟啉单胞菌、口腔链球菌和Veillonella dispar。对这些生物膜进行 1、3 和 5 分钟的 LTP 处理,LTP 喷嘴与样品的距离为 3 或 10 毫米。对照组包括在钛盘或植入物上形成的生物膜,这些生物膜未接受任何处理;在 3 或 10 毫米的距离内暴露于氩气流 1、3 或 5 分钟;使用 14 μg/mL 阿莫西林、140 μg/mL 甲硝唑或两者的混合物 1 分钟;使用 0.12% 洗必泰 (CHX) 处理 1 分钟。对于种植体,用 0.12% CHX 0.12% 处理 21 天的生物膜 1 分钟,然后用 LTP 处理 1 分钟,每个象限的距离为 3 毫米。通过细菌计数和共聚焦激光扫描显微镜评估生物膜的活力。研究了 LTP 对受牙龈脓胞污染的重建口腔上皮(ROE)的影响,评估了细胞毒性、细胞活力和组织学。结果表明,与未经处理的对照组相比,在距离为 3 毫米或 10 毫米处接触 LTP 1 分钟可显著降低种植体和牙盘上的细菌数量(p < 0.017)。与未处理的污染对照组相比,LTP 污染 12 小时后产生的细胞毒性水平较低(p = 0.038),细胞存活率不受 LTP 影响(p ≥ 0.05);因此,经 LTP 处理的样品可安全用于组织应用,处理后细胞毒性较低,细胞存活率较高,组织学定性分析也验证了这些结果。总之,研究结果表明,暴露 1 分钟的 LTP 可以成功消毒钛盘和种植体上成熟的种植体周围炎多菌种生物膜。此外,研究还验证了 LTP 对 ROE 的安全性,表明它有可能成为种植体周围炎的辅助治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tissue-Safe Low-Temperature Plasma Treatment for Effective Management of Mature Peri-Implantitis Biofilms on Titanium Surfaces.

The unique screw-shape design and microstructure of implants pose a challenge for mechanical debridement in removing biofilms. Biofilms exhibit increased resistance to antimicrobials relative to single planktonic cells, emphasizing the need for effective biofilm removal during periodontal therapy for peri-implantitis treatment. To tackle this issue, our team evaluated the effectiveness of low-temperature plasma (LTP) for disinfecting titanium discs contaminated with multispecies biofilms associated with peri-implantitis, specifically focusing on biofilms matured for 14 and 21 days as well as biofilms that had formed on Straumann Ti-SLA implants for 21 days. The biofilms included Actinomyces naeslundii, Porphyromonas gingivalis, Streptococcus oralis, and Veillonella dispar, which were grown in anaerobic conditions. These biofilms were subjected to LTP treatment for 1, 3, and 5 min, using distances of 3 or 10 mm from the LTP nozzle to the samples. Control groups included biofilms formed on Ti discs or implants that received no treatment, exposure to argon flow at 3 or 10 mm of distance for 1, 3, or 5 min, application for 1 min of 14 μg/mL amoxicillin, 140 μg/mL metronidazole, or a blend of both, and treatment with 0.12% chlorhexidine (CHX) for 1 min. For the implants, 21-day-old biofilms were treated with 0.12% CHX 0.12% for 1 min and LTP for 1 min at a distance of 3 mm for each quadrant. Biofilm viability was assessed through bacterial counting and confocal laser scanning microscopy. The impact of LTP was investigated on reconstituted oral epithelia (ROE) contaminated with P. gingivalis, evaluating cytotoxicity, cell viability, and histology. The results showed that a 1 min exposure to LTP at distances of 3 or 10 mm significantly lowered bacterial counts on implants and discs compared to the untreated controls (p < 0.017). LTP exposure yielded lower levels of cytotoxicity relative to the untreated contaminated control after 12 h of contamination (p = 0.038), and cell viability was not affected by LTP (p ≥ 0.05); thus, LTP-treated samples were shown to be safe for tissue applications, with low cytotoxicity and elevated cell viability post-treatment, and these results were validated by qualitative histological analysis. In conclusion, the study's results support the effectiveness of 1 min LTP exposure in successfully disinfecting mature peri-implantitis multispecies biofilms on titanium discs and implants. Moreover, it validated the safety of LTP on ROE, suggesting its potential as an adjunctive treatment for peri-implantitis.

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来源期刊
ACS Biomaterials Science & Engineering
ACS Biomaterials Science & Engineering Materials Science-Biomaterials
CiteScore
10.30
自引率
3.40%
发文量
413
期刊介绍: ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture
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