Li Wang, Hongyu Wu, Caihong Wang, Yunfei Ma, Zheng Xiang
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Triple-template surface imprinted magnetic polymers for wide-coverage extraction of steroid hormones from human serum.
In this study, novel triple-template magnetic nanospheres by surface imprinting, called triple-template magnetic molecularly imprinted polymers (tri-MMIPs), were prepared for the detection of steroid hormones in human serum. The polymers were constructed by Fe3O4 as support, estrone (E1), progesterone (PROG), and estradiol (E2) as triple templates, acrylic acid (AA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The resulting tri-MMIPs were further characterized and applied as sorbents in human serum pretreatment. Coupled with ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-MS/MS) profiling after derivatization, 15 steroids (E1, 6-ketoE1, 4-OHE1, 16α-OHE1, DHEA, 4-MeOE1, PROG, PREG, 17-OHPROG, 7-DHE2, E2, 2-OHE2, 16-epiE3, E3, 2-MeOE2) were successfully quantified. The developed method exhibited a good recovery (78.9-114%) and low limit of quantitation (LLOQ, 5-10 pg/mL), which indicates a great potential for application in the analysis of multiple steroid hormones in complex biological samples.
期刊介绍：
Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods.
This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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