Tuany R Schmidt, Belkiss C Mármora, Fernanda T Brochado, Lucas Gonçalves, Paloma S Campos, Marcelo L Lamers, Aurigena A de Araújo, Caroline A C X de Medeiros, Susana B Ribeiro, Marco A T Martins, Emily F S Pilar, Manoela D Martins, Vivian P Wagner
{"title":"红色发光二极管对皮肤愈合的影响：体外和体内实验研究。","authors":"Tuany R Schmidt, Belkiss C Mármora, Fernanda T Brochado, Lucas Gonçalves, Paloma S Campos, Marcelo L Lamers, Aurigena A de Araújo, Caroline A C X de Medeiros, Susana B Ribeiro, Marco A T Martins, Emily F S Pilar, Manoela D Martins, Vivian P Wagner","doi":"10.1016/j.abd.2024.02.008","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The clinical advantages of light-emitting diode (LED) therapy in skin healing and its underlying mechanism remain subjects of ongoing debate.</p><p><strong>Objective: </strong>This study aims to explore the impact of LED therapy on normal skin keratinocytes (HaCaT) and in the repair of full-thickness dorsal wounds in Wistar rats.</p><p><strong>Methods: </strong>HaCaT cell viability (SRB assay) and migration (scratch assay) were assessed under LED therapy, comparing stress conditions (2.5% FBS) with sham irradiation and optimal conditions (10% FBS). In vivo, 50 rats with induced wounds were divided into Sham and LED (daily treatment) groups. Euthanasia occurred at 3, 5, 10, 14, and 21 days for clinical, morphological, oxidative stress (MDA, SOD, and GSH), and cytokine analyses (IL-1β, IL-10, TNF-α).</p><p><strong>Results: </strong>LED therapy significantly enhanced keratinocytes viability compared to sham irradiation, with minimal impact on cell migration. Clinical benefits were prominent on day 10, influencing inflammation progression and resolution on days 3 and 10. Re-epithelization remained unaffected. Reduced MDA and increased GSH levels were observed throughout, while SOD levels varied temporally. Notably, on day 10, LED significantly decreased IL-1β, IL-10, and TNF-α.</p><p><strong>Study limitations: </strong>Although translational, clinical trial confirmation of observed benefits is warranted.</p><p><strong>Conclusions: </strong>LED therapy expedites cutaneous healing in the experimental model, primarily modulating inflammation and enhancing antioxidant activity.</p>","PeriodicalId":7787,"journal":{"name":"Anais brasileiros de dermatologia","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Red light-emitting diode on skin healing: an in vitro and in vivo experimental study.\",\"authors\":\"Tuany R Schmidt, Belkiss C Mármora, Fernanda T Brochado, Lucas Gonçalves, Paloma S Campos, Marcelo L Lamers, Aurigena A de Araújo, Caroline A C X de Medeiros, Susana B Ribeiro, Marco A T Martins, Emily F S Pilar, Manoela D Martins, Vivian P Wagner\",\"doi\":\"10.1016/j.abd.2024.02.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The clinical advantages of light-emitting diode (LED) therapy in skin healing and its underlying mechanism remain subjects of ongoing debate.</p><p><strong>Objective: </strong>This study aims to explore the impact of LED therapy on normal skin keratinocytes (HaCaT) and in the repair of full-thickness dorsal wounds in Wistar rats.</p><p><strong>Methods: </strong>HaCaT cell viability (SRB assay) and migration (scratch assay) were assessed under LED therapy, comparing stress conditions (2.5% FBS) with sham irradiation and optimal conditions (10% FBS). In vivo, 50 rats with induced wounds were divided into Sham and LED (daily treatment) groups. Euthanasia occurred at 3, 5, 10, 14, and 21 days for clinical, morphological, oxidative stress (MDA, SOD, and GSH), and cytokine analyses (IL-1β, IL-10, TNF-α).</p><p><strong>Results: </strong>LED therapy significantly enhanced keratinocytes viability compared to sham irradiation, with minimal impact on cell migration. Clinical benefits were prominent on day 10, influencing inflammation progression and resolution on days 3 and 10. Re-epithelization remained unaffected. Reduced MDA and increased GSH levels were observed throughout, while SOD levels varied temporally. Notably, on day 10, LED significantly decreased IL-1β, IL-10, and TNF-α.</p><p><strong>Study limitations: </strong>Although translational, clinical trial confirmation of observed benefits is warranted.</p><p><strong>Conclusions: </strong>LED therapy expedites cutaneous healing in the experimental model, primarily modulating inflammation and enhancing antioxidant activity.</p>\",\"PeriodicalId\":7787,\"journal\":{\"name\":\"Anais brasileiros de dermatologia\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-11-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anais brasileiros de dermatologia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.abd.2024.02.008\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anais brasileiros de dermatologia","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.abd.2024.02.008","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}
Red light-emitting diode on skin healing: an in vitro and in vivo experimental study.
Background: The clinical advantages of light-emitting diode (LED) therapy in skin healing and its underlying mechanism remain subjects of ongoing debate.
Objective: This study aims to explore the impact of LED therapy on normal skin keratinocytes (HaCaT) and in the repair of full-thickness dorsal wounds in Wistar rats.
Methods: HaCaT cell viability (SRB assay) and migration (scratch assay) were assessed under LED therapy, comparing stress conditions (2.5% FBS) with sham irradiation and optimal conditions (10% FBS). In vivo, 50 rats with induced wounds were divided into Sham and LED (daily treatment) groups. Euthanasia occurred at 3, 5, 10, 14, and 21 days for clinical, morphological, oxidative stress (MDA, SOD, and GSH), and cytokine analyses (IL-1β, IL-10, TNF-α).
Results: LED therapy significantly enhanced keratinocytes viability compared to sham irradiation, with minimal impact on cell migration. Clinical benefits were prominent on day 10, influencing inflammation progression and resolution on days 3 and 10. Re-epithelization remained unaffected. Reduced MDA and increased GSH levels were observed throughout, while SOD levels varied temporally. Notably, on day 10, LED significantly decreased IL-1β, IL-10, and TNF-α.
Study limitations: Although translational, clinical trial confirmation of observed benefits is warranted.
Conclusions: LED therapy expedites cutaneous healing in the experimental model, primarily modulating inflammation and enhancing antioxidant activity.
期刊介绍：
The journal is published bimonthly and is devoted to the dissemination of original, unpublished technical-scientific study, resulting from research or reviews of dermatological topics and related matters. Exchanges with other publications may be accepted.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
