在体外培养中添加天然盐溴碘矿泉水可增强人体原生皮肤成纤维细胞的增殖。

IF 2.1 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in Clinical and Experimental Medicine Pub Date : 2024-11-12 DOI:10.17219/acem/192227

Marco Saler, Ottavia Ferraro, Angela Faga, Debora Sansotta, Simona Villani, Giovanni Nicoletti

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引用次数: 0

摘要

背景：一些矿泉水对伤口愈合的有利再生作用早已得到经验证明：本实验研究的目的是调查一种意大利盐溴碘矿泉水（意大利里瓦纳扎诺）对体外人类原生成纤维细胞培养模型的影响，以确定任何潜在的再生作用：- 用蒸馏水重新配制的杜氏改良老鹰培养基（DMEM）（对照组）； - 用从泉水中收集的过滤矿泉水重新配制的杜氏改良老鹰培养基（DMEM）； - 用从浴疗设施中收集的过滤矿泉水重新配制的杜氏改良老鹰培养基（DMEM）； - 用从浴疗设施中收集的过滤加热矿泉水重新配制的杜氏改良老鹰培养基（DMEM）；- 部分用从泉水中收集的不同浓度（10%、20%、30%、40%、50%）的过滤矿泉水代替 DMEM； - 部分用从浴疗设施中收集的不同浓度（10%、20%、30%、40%、50%）的过滤加热矿泉水代替 DMEM； - 部分用从浴疗设施中收集的不同浓度（10%、20%、30%、40%、50%）的过滤矿泉水代替 DMEM。用 XTT 显微培养四氮唑检测法染色后，用分光光度法评估细胞的增殖和活力。在 24、48 和 72 小时内对每种实验条件进行统计分析：结果：用部分从泉水中收集的过滤矿泉水代替 DMEM 培养成纤维细胞，结果最好，范围在 20-50% 之间：我们的研究结果表明，里瓦纳扎诺盐溴碘矿泉水对体外培养的人体原生成纤维细胞有刺激作用。从泉水中采集的矿泉水的这种活性最为明显，而从浴疗设施中采集的矿泉水的这种活性则有所下降。这些发现为伤口愈合和浴疗法的临床应用奠定了基础。

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Enhancement of human native skin fibroblast proliferation in natural salso-bromo-iodic mineral water added to in vitro culture.

Background: The favorable regenerative effects of some mineral waters on wound healing have long been empirically demonstrated.

Objectives: The aim of this experimental study is to investigate the effects of an Italian salso-bromo-iodic mineral water (Rivanazzano, Italy) on an in vitro human native fibroblast culture model to identify any potential regenerative actions.

Material and methods: Human native fibroblasts were cultured under different experimental conditions: - Dulbecco's modified Eagle's medium (DMEM) reconstituted with distilled water (control); - DMEM reconstituted with filtered mineral water collected from the spring; - DMEM reconstituted with filtered mineral water collected at the balneotherapy facility; - DMEM reconstituted with filtered, heated mineral water collected at the balneotherapy facility; - DMEM partially replaced with filtered mineral water collected from the spring at different concentrations (10%, 20%, 30%, 40%, 50%); - DMEM partially replaced with filtered, heated mineral water collected at the balneotherapy facility at different concentrations (10%, 20%, 30%, 40%, 50%); - DMEM partially replaced with filtered mineral water collected at the balneotherapy facility at different concentrations (10%, 20%, 30%, 40%, 50%). Cell proliferation and viability were evaluated using spectrophotometric analysis following staining with the XTT Microculture Tetrazolium Assay. Statistical analyses were performed for each experimental condition at 24, 48 and 72 h.

Results: The best outcomes were observed in fibroblasts cultured with DMEM partially replaced with filtered mineral water collected from the spring, within the range of 20-50%.

Conclusions: Our research results showed that Rivanazzano salso-bromo-iodic mineral water has a stimulating effect on in vitro human native fibroblast cultures. This activity was most pronounced with water collected from the spring, and it decreased with water collected at the balneotherapy facilities. These findings could form the basis for clinical applications in wound healing and balneotherapy.

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来源期刊

Advances in Clinical and Experimental Medicine MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

3.70

自引率

4.80%

发文量

153

审稿时长

6-12 weeks

期刊介绍： Advances in Clinical and Experimental Medicine has been published by the Wroclaw Medical University since 1992. Establishing the medical journal was the idea of Prof. Bogumił Halawa, Chair of the Department of Cardiology, and was fully supported by the Rector of Wroclaw Medical University, Prof. Zbigniew Knapik. Prof. Halawa was also the first editor-in-chief, between 1992-1997. The journal, then entitled "Postępy Medycyny Klinicznej i Doświadczalnej", appeared quarterly. Prof. Leszek Paradowski was editor-in-chief from 1997-1999. In 1998 he initiated alterations in the profile and cover design of the journal which were accepted by the Editorial Board. The title was changed to Advances in Clinical and Experimental Medicine. Articles in English were welcomed. A number of outstanding representatives of medical science from Poland and abroad were invited to participate in the newly established International Editorial Staff. Prof. Antonina Harłozińska-Szmyrka was editor-in-chief in years 2000-2005, in years 2006-2007 once again prof. Leszek Paradowski and prof. Maria Podolak-Dawidziak was editor-in-chief in years 2008-2016. Since 2017 the editor-in chief is prof. Maciej Bagłaj. Since July 2005, original papers have been published only in English. Case reports are no longer accepted. The manuscripts are reviewed by two independent reviewers and a statistical reviewer, and English texts are proofread by a native speaker. The journal has been indexed in several databases: Scopus, Ulrich’sTM International Periodicals Directory, Index Copernicus and since 2007 in Thomson Reuters databases: Science Citation Index Expanded i Journal Citation Reports/Science Edition. In 2010 the journal obtained Impact Factor which is now 1.179 pts. Articles published in the journal are worth 15 points among Polish journals according to the Polish Committee for Scientific Research and 169.43 points according to the Index Copernicus. Since November 7, 2012, Advances in Clinical and Experimental Medicine has been indexed and included in National Library of Medicine’s MEDLINE database. English abstracts printed in the journal are included and searchable using PubMed http://www.ncbi.nlm.nih.gov/pubmed.