Shuk-Ching Wong, Jonathan Hon-Kwan Chen, Pui-Hing Chau, Wai-On Tam, Germaine Kit-Ming Lam, Lithia Lai-Ha Yuen, Wan-Mui Chan, Allen Wing-Ho Chu, Jonathan Daniel Ip, Hoi-Wah Tsoi, Simon Yung-Chun So, Kelvin Hei-Yeung Chiu, Kwok-Yung Yuen, Kelvin Kai-Wang To, Vincent Chi-Chung Cheng
{"title":"追踪空气中的 SARS-CoV-2 RNA：从医务室爆发的 COVID-19 事件中汲取的教训。","authors":"Shuk-Ching Wong, Jonathan Hon-Kwan Chen, Pui-Hing Chau, Wai-On Tam, Germaine Kit-Ming Lam, Lithia Lai-Ha Yuen, Wan-Mui Chan, Allen Wing-Ho Chu, Jonathan Daniel Ip, Hoi-Wah Tsoi, Simon Yung-Chun So, Kelvin Hei-Yeung Chiu, Kwok-Yung Yuen, Kelvin Kai-Wang To, Vincent Chi-Chung Cheng","doi":"10.1016/j.ajic.2024.10.035","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The duration and magnitude of SARS-CoV-2 air dispersal during nosocomial outbreaks remain uncertain. This study evaluates the impact of mobile modular high-efficiency-particulate-air filter units (MMHUs) on SARS-CoV-2 air dispersal.</p><p><strong>Methods: </strong>We investigated a nosocomial COVID-19 outbreak in an infirmary unit. The viral load (VL) of SARS-CoV-2 in air samples was correlated with the VL in nasopharyngeal swabs (NPS) with or without MMHU. These samples underwent whole genome sequencing (WGS) and phylogenetic analysis.</p><p><strong>Results: </strong>Upon outbreak declaration (2 August 2024, Day 0), 44 (69.8%) of 63 patients acquired COVID-19 in Ward 2B (19 male) and 2C (25 female) by Day 4. The VL of SARS-CoV-2 remained detectable in air until Day 11 (2B) and Day 20 (2C). The VL of air samples was significantly correlated with the VL in NPS collected on Days 5,7,10, and 13 in 2C (r= -0.975, p=0.004). Using MMHU, the mean daily ratio of SARS-CoV-2 RNA (copies/1,000L of air/patient) in 2B was five times lower than in 2C from Days 5 to 10. WGS revealed all 41 tested strains belonged to the Omicron variant, KP.3.1.1, phylogenetically related to the prevailing community strains.</p><p><strong>Conclusion: </strong>Using MMHU mitigates the duration and magnitude of SARS-CoV-2 air dispersal during nosocomial outbreak.</p>","PeriodicalId":7621,"journal":{"name":"American journal of infection control","volume":" ","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tracking SARS-CoV-2 RNA in the Air: Lessons from a COVID-19 Outbreak in an Infirmary Unit.\",\"authors\":\"Shuk-Ching Wong, Jonathan Hon-Kwan Chen, Pui-Hing Chau, Wai-On Tam, Germaine Kit-Ming Lam, Lithia Lai-Ha Yuen, Wan-Mui Chan, Allen Wing-Ho Chu, Jonathan Daniel Ip, Hoi-Wah Tsoi, Simon Yung-Chun So, Kelvin Hei-Yeung Chiu, Kwok-Yung Yuen, Kelvin Kai-Wang To, Vincent Chi-Chung Cheng\",\"doi\":\"10.1016/j.ajic.2024.10.035\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The duration and magnitude of SARS-CoV-2 air dispersal during nosocomial outbreaks remain uncertain. This study evaluates the impact of mobile modular high-efficiency-particulate-air filter units (MMHUs) on SARS-CoV-2 air dispersal.</p><p><strong>Methods: </strong>We investigated a nosocomial COVID-19 outbreak in an infirmary unit. The viral load (VL) of SARS-CoV-2 in air samples was correlated with the VL in nasopharyngeal swabs (NPS) with or without MMHU. These samples underwent whole genome sequencing (WGS) and phylogenetic analysis.</p><p><strong>Results: </strong>Upon outbreak declaration (2 August 2024, Day 0), 44 (69.8%) of 63 patients acquired COVID-19 in Ward 2B (19 male) and 2C (25 female) by Day 4. The VL of SARS-CoV-2 remained detectable in air until Day 11 (2B) and Day 20 (2C). The VL of air samples was significantly correlated with the VL in NPS collected on Days 5,7,10, and 13 in 2C (r= -0.975, p=0.004). Using MMHU, the mean daily ratio of SARS-CoV-2 RNA (copies/1,000L of air/patient) in 2B was five times lower than in 2C from Days 5 to 10. WGS revealed all 41 tested strains belonged to the Omicron variant, KP.3.1.1, phylogenetically related to the prevailing community strains.</p><p><strong>Conclusion: </strong>Using MMHU mitigates the duration and magnitude of SARS-CoV-2 air dispersal during nosocomial outbreak.</p>\",\"PeriodicalId\":7621,\"journal\":{\"name\":\"American journal of infection control\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-11-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American journal of infection control\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ajic.2024.10.035\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of infection control","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ajic.2024.10.035","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Tracking SARS-CoV-2 RNA in the Air: Lessons from a COVID-19 Outbreak in an Infirmary Unit.
Background: The duration and magnitude of SARS-CoV-2 air dispersal during nosocomial outbreaks remain uncertain. This study evaluates the impact of mobile modular high-efficiency-particulate-air filter units (MMHUs) on SARS-CoV-2 air dispersal.
Methods: We investigated a nosocomial COVID-19 outbreak in an infirmary unit. The viral load (VL) of SARS-CoV-2 in air samples was correlated with the VL in nasopharyngeal swabs (NPS) with or without MMHU. These samples underwent whole genome sequencing (WGS) and phylogenetic analysis.
Results: Upon outbreak declaration (2 August 2024, Day 0), 44 (69.8%) of 63 patients acquired COVID-19 in Ward 2B (19 male) and 2C (25 female) by Day 4. The VL of SARS-CoV-2 remained detectable in air until Day 11 (2B) and Day 20 (2C). The VL of air samples was significantly correlated with the VL in NPS collected on Days 5,7,10, and 13 in 2C (r= -0.975, p=0.004). Using MMHU, the mean daily ratio of SARS-CoV-2 RNA (copies/1,000L of air/patient) in 2B was five times lower than in 2C from Days 5 to 10. WGS revealed all 41 tested strains belonged to the Omicron variant, KP.3.1.1, phylogenetically related to the prevailing community strains.
Conclusion: Using MMHU mitigates the duration and magnitude of SARS-CoV-2 air dispersal during nosocomial outbreak.
期刊介绍：
AJIC covers key topics and issues in infection control and epidemiology. Infection control professionals, including physicians, nurses, and epidemiologists, rely on AJIC for peer-reviewed articles covering clinical topics as well as original research. As the official publication of the Association for Professionals in Infection Control and Epidemiology (APIC)
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