用于快速发现真菌粗提取物中氮杂天然产物的质量标记胺化探针

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2024-12-03 DOI:10.1039/d4cc05122j

Victor Flon , Coralie Pavesi , Samuel Oger , Stéphane Leleu , Pascal Retailleau , Laurence K. Jennings , Soizic Prado , Xavier Franck

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引用次数: 0

摘要

基于反应性的筛选（RBS）被用来筛选真菌粗提取物中新的唑类天然产物。我们设计了一种由亲核伯胺和同位素质量标签组成的探针，其对萘类化合物具有反应活性。将探针加入到真菌复合物的粗提取物中，再配合去复制分析工具（如卤素检测器和分子网络），就能轻松检测出萘类化合物。利用这一 RBS 方案，分离出了新的萘甲内酯并确定了其结构特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Mass-tagged aminated probes for rapid discovery of azaphilic natural products in fungal crude extracts†

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Mass-tagged aminated probes for rapid discovery of azaphilic natural products in fungal crude extracts†

Reactivity-based screening (RBS) was used to screen fungal crude extracts for the presence of new azaphilic natural products. A probe composed of a nucleophilic primary amine and an isotopic mass tag was designed for its reactivity towards azaphilic compounds. Addition of the probe to crude extracts of fungal complexes, together with analytical tools for dereplication such as haloseeker and molecular networking, allowed easy detection of azaphilic compounds. The exploitation of this RBS protocol led to the isolation and structural characterization of new azaphilones.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.