利用聚呋喃乙烯可溶性支架为 PEF 酶的定向进化建立酶基准

IF 3.7 3区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Mikel Dolz, Dianelis T. Monterrey, Felice Quartinello, Patricia Gomez de Santos, Ivan Mateljak, Alessandro Pellis, Georg Guebitz, Javier Viña-González* and Miguel Alcalde*, 
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引用次数: 0

摘要

塑料垃圾是工业化世界的一大威胁,也是生物塑料研究的动力。作为化石来源的聚对苯二甲酸乙二醇酯(PET)的可行替代品,生物基聚呋喃乙烯酯(PEF)的成功与否将取决于能否设计出有效的酶来分解它,从而帮助其循环利用。在这里,一组真菌和细菌角叉菜胶酶在基于酿酒酵母和糊粉菌的串联酵母表达系统中进行了功能表达。用可溶性 PEF 模型支架测试了酶组的活性,观察到其与真实 PEF 粉末降解的相关性。基于 PEF 支架的呋喃-2,5-二甲酸二乙酯开发了一种高通量比色筛选测定法,为未来 PEF 酶的定向进化活动奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enzyme Benchmarking with Polyethylene Furanoate Soluble Scaffolds for Directed Evolution of PEFases

Plastic waste is a major threat in our industrialized world and is driving research into bioplastics. The success of biobased polyethylene furanoate (PEF) as a viable alternative to polyethylene terephthalate (PET) of fossil origin will depend on designing effective enzymes to break it down, aiding its recycling. Here, a panel of fungal and bacterial cutinases were functionally expressed in a tandem yeast expression system based on Saccharomyces cerevisiae and Pichia pastoris. The activity of the enzyme panel was tested with soluble PEF model scaffolds, observing a correlation with the degradation of real PEF powder. A high-throughput colorimetric screening assay based on the PEF scaffold diethyl furan-2,5-dicarboxylate was developed, establishing the basis for future directed evolution campaigns of PEFases.

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来源期刊
ACS Omega
ACS Omega Chemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍: ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.
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