使用 MEA 和尖锐电极技术研究 hPSC 衍生的三维心脏器官组织的电生理特性。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-11-07 DOI:10.1016/j.xpro.2024.103406

Ravichandra Venkateshappa, Zehra Yildirim, Shane R Zhao, Matthew A Wu, Francesca Vacante, Oscar J Abilez, Joseph C Wu

{"title":"使用 MEA 和尖锐电极技术研究 hPSC 衍生的三维心脏器官组织的电生理特性。","authors":"Ravichandra Venkateshappa, Zehra Yildirim, Shane R Zhao, Matthew A Wu, Francesca Vacante, Oscar J Abilez, Joseph C Wu","doi":"10.1016/j.xpro.2024.103406","DOIUrl":null,"url":null,"abstract":"Continuing advancements in human pluripotent stem cell (hPSC)-derived complex three-dimensional (3D) cardiac tissues require the development of novel technologies or adaptation of existing technologies to understand the physiology of the derived 3D cardiac tissues. In this protocol, we describe the use of multielectrode array (MEA) and sharp electrode electrophysiology techniques to investigate the electrical properties of 3D cardiac organoids. This protocol deciphers the electrical behavior of 3D cardiac organoids at both the single-cell level and tissue level.","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103406"},"PeriodicalIF":1.3000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584947/pdf/","citationCount":"0","resultStr":"{\"title\":\"Protocol to study electrophysiological properties of hPSC-derived 3D cardiac organoids using MEA and sharp electrode techniques.\",\"authors\":\"Ravichandra Venkateshappa, Zehra Yildirim, Shane R Zhao, Matthew A Wu, Francesca Vacante, Oscar J Abilez, Joseph C Wu\",\"doi\":\"10.1016/j.xpro.2024.103406\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Continuing advancements in human pluripotent stem cell (hPSC)-derived complex three-dimensional (3D) cardiac tissues require the development of novel technologies or adaptation of existing technologies to understand the physiology of the derived 3D cardiac tissues. In this protocol, we describe the use of multielectrode array (MEA) and sharp electrode electrophysiology techniques to investigate the electrical properties of 3D cardiac organoids. This protocol deciphers the electrical behavior of 3D cardiac organoids at both the single-cell level and tissue level.\",\"PeriodicalId\":34214,\"journal\":{\"name\":\"STAR Protocols\",\"volume\":\"5 4\",\"pages\":\"103406\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584947/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"STAR Protocols\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.xpro.2024.103406\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/11/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"STAR Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xpro.2024.103406","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/7 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

人类多能干细胞（hPSC）衍生的复杂三维（3D）心脏组织的不断进步需要开发新技术或调整现有技术，以了解衍生的三维心脏组织的生理学。在本方案中，我们介绍了使用多电极阵列（MEA）和尖锐电极电生理学技术来研究三维心脏器官组织的电特性。该方案在单细胞水平和组织水平上解密了三维心脏器官组织的电行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Protocol to study electrophysiological properties of hPSC-derived 3D cardiac organoids using MEA and sharp electrode techniques.

Continuing advancements in human pluripotent stem cell (hPSC)-derived complex three-dimensional (3D) cardiac tissues require the development of novel technologies or adaptation of existing technologies to understand the physiology of the derived 3D cardiac tissues. In this protocol, we describe the use of multielectrode array (MEA) and sharp electrode electrophysiology techniques to investigate the electrical properties of 3D cardiac organoids. This protocol deciphers the electrical behavior of 3D cardiac organoids at both the single-cell level and tissue level.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊