利用硅烷包裹的磁性纳米颗粒分离 B 细胞

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
International Journal of Biomaterials Pub Date : 2024-10-30 eCollection Date: 2024-01-01 DOI:10.1155/2024/8286525
Amir Hossein Haghighi, Abolfazl Ghaderian, Esmaeil Mirzaei
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引用次数: 0

摘要

涂层纳米粒子在生物应用中最重要的优势和应用之一是用于分离不同类型的细胞,以诊断和治疗各种疾病。因此,在这项研究工作中,研究了使用磁性氧化铁纳米粒子分离和富集 B 细胞的可能性。为此,首先在磁性纳米粒子上涂覆(3-氨基丙基)三乙氧基硅烷,使其具有亲水性并防止结块,然后与 FITC 抗人 CD20 抗体反应并使其具有生物相容性。这些含有抗体的纳米颗粒已被用于从淋巴细胞中分离 B 细胞。透射电子显微镜(TEM)和振动样品磁力计(VSM)测试用于检测纳米粒子的磁性和涂层。流式细胞仪和荧光显微镜测试用于检查抗体与纳米颗粒的结合情况。此外,还使用流式细胞仪检测细胞分离的程度。结果表明,与 450 μL 抗体(T450)反应的纳米粒子在分离 B 细胞方面的表现优于其他纳米粒子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation of B Cells Using Silane-Coated Magnetic Nanoparticles.

One of the most important advantages and applications of coated nanoparticles in biological applications is their use in isolating different types of cells to diagnose and treat all types of diseases. Therefore, in this research work, the possibility of isolation and enrichment of B cells using magnetic iron oxide nanoparticles have been investigated. In this regard, magnetic nanoparticles are first coated with (3-aminopropyl)triethoxysilane to make them hydrophilic and prevent their clumping, then reacted with and rendered biocompatible by FITC anti-human CD20 antibody. These nanoparticles containing antibodies have been used to isolate B cells from the lymphatic cells. Transmission electron microscopy (TEM) and vibrating-sample magnetometry (VSM) tests were used to check the magnetic properties and coating of nanoparticles. The flow cytometry and fluorescent microscopy tests are used to check antibody binding to nanoparticles. Moreover, flow cytometry tests were used to check the extent of cell separation. Results show that nanoparticles reacted with 450 μL of antibody (T450) performed better than other nanoparticles in isolating B cells.

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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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