普通感冒冠状病毒 229E 在慢性鼻炎伴息肉患者的人鼻上皮细胞中诱导更高的干扰素刺激基因反应

IF 2.5 3区 医学 Q1 OTORHINOLARYNGOLOGY
Elizabeth A Sell, Li Hui Tan, David M Renner, Jennifer Douglas, Robert J Lee, Michael A Kohanski, John V Bosso, David W Kennedy, James N Palmer, Nithin D Adappa, Susan R Weiss, Noam A Cohen
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引用次数: 0

摘要

背景:长期以来,病毒感染一直与慢性鼻炎伴鼻息肉(CRSwNP)的发病有关。鉴于普通感冒冠状病毒 229E(HCoV229E)的广泛暴露,我们试图研究 CRSwNP 患者的气液界面(ALI)培养物中 HCoV-229E 如何被清除并刺激干扰素途径:本研究旨在确定与对照组相比,CRSwNP 患者供体的 ALI 培养物中病毒清除率和 ISG 表达量是否有所不同:方法:与无 CRS 患者(对照组)相比,采用斑块检测法对来自 CRSwNP 患者的受感染气液界面(ALI)培养物释放的传染性病毒进行量化。此外,还通过 RT-qPCR 定量了感染 HCoV-229E 后干扰素刺激基因(ISGs)mRNA 的模拟和诱导水平:结果:通过斑块检测对感染性病毒进行定量发现,CRSwNP ALI 培养物同样易受 HCoV-229E 感染,而且令人惊讶的是,病毒滴度的下降速度明显快于对照 ALI 培养物。我们进一步证明,与对照 ALIs 相比,CRSwNP ALIs 感染病毒后,病毒清除速度加快与至少 4 种 ISGs 的 mRNA 表达增加有关:这项研究自相矛盾地证明,与对照组相比,CRSwNP 患者的 ALI 培养物清除普通感冒 HCoV-229E 病毒的效率更高。我们还证明,CRSwNP 感染 HCoV-229E 病毒后,ISG mRNA 的表达明显增加。这些发现要求我们进一步研究干扰素信号传导未受损对 CRSwNP 患者 2 型炎症环境的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Common Cold Coronavirus 229E Induces Higher Interferon Stimulating Gene Responses in Human Nasal Epithelial Cells from Patients with Chronic Rhinosinusitis with Polyposis.

Background: Viral infections have long been implicated in the development of chronic rhinosinusitis with nasal polyps (CRSwNP). Given widespread exposure to the common cold coronavirus 229E (HCoV229E), we sought to investigate how HCoV-229E is cleared and stimulates interferon pathways in air-liquid interface (ALI) cultures from patients with CRSwNP.

Objective: The objective of this study was to identify whether viral clearance and ISG expression is different in ALI cultures from donors with CRSwNP compared with controls.

Methods: Plaque assays were used to quantify infectious virus released by infected air-liquid interface (ALI) cultures derived from patients with CRSwNP compared to patients without CRS (controls). Additionally, mock and induced levels of Interferon Stimulated Genes (ISGs) mRNA following HCoV-229E infection were quantified by RT-qPCR.

Results: Quantification of infectious virus by plaque assay reveals that CRSwNP ALI cultures were equally susceptible to HCoV-229E infection, and surprisingly viral titers dropped significantly faster than in the control ALI cultures. We further demonstrate that this accelerated viral clearance correlates with increased mRNA expression of at least 4 ISGs following viral infection in the CRSwNP ALIs compared to the control ALIs.

Conclusion: This study paradoxically demonstrates that ALI cultures from patients with CRSwNP are more efficient at clearing the common cold HCoV-229E virus compared to controls. We also demonstrate significantly increased ISG mRNA expression following HCoV-229E infection in CRSwNP. These findings call for further investigation into the effect of unimpaired interferon signaling on the type 2 inflammatory environment in patients with CRSwNP.

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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
82
审稿时长
4-8 weeks
期刊介绍: The American Journal of Rhinology & Allergy is a peer-reviewed, scientific publication committed to expanding knowledge and publishing the best clinical and basic research within the fields of Rhinology & Allergy. Its focus is to publish information which contributes to improved quality of care for patients with nasal and sinus disorders. Its primary readership consists of otolaryngologists, allergists, and plastic surgeons. Published material includes peer-reviewed original research, clinical trials, and review articles.
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