比较强迫运动和自愿运动对雄性大鼠大脑单胺类水平、焦虑样行为以及明暗阶段生理指标的影响。

IF 2.6 3区心理学 Q2 BEHAVIORAL SCIENCES

Behavioural Brain Research Pub Date : 2024-11-06 DOI:10.1016/j.bbr.2024.115321

Daisuke Matsunaga , Hikaru Nakagawa , Takayuki Ishiwata

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引用次数: 0

摘要

目的：体育锻炼可改善身心健康；然而，自愿锻炼和强制锻炼方案之间的差异尚不清楚。此外，有关测试时间差异（如明暗阶段）对运动类型的影响的知识也很有限。我们研究了长期强迫和自愿车轮跑对大脑单胺类物质（5-HT：5-羟色胺；DA：多巴胺；NA：去甲肾上腺素）水平变化、焦虑样行为以及明暗阶段生理应激反应的影响：将成年雄性 Wistar 大鼠平均随机分配到四组：静坐对照组、自愿运动组（在车轮上自由奔跑，V-EX）、自愿限制运动组（每天只能使用车轮 1 小时，VL-EX）和强迫运动组（在电动车轮上奔跑，F-EX）。每组大鼠又分为暗实验条件组和光实验条件组。4 周后，大鼠接受开放场地测试。在大脑中与行为、认知、焦虑和压力有关的主要神经细胞体和投射区测量了单胺类物质及其代谢物的水平：结果：在表现出焦虑样行为的 F-EX 大鼠中观察到肾上腺肥大和体温升高（运动期除外）。与 VL-EX 组和其他组的大鼠相比，V-EX 组大鼠特定区域的单胺及其代谢物，特别是 5-HT 能和 DA 能系统的水平发生了显著变化。这些差异仅在黑暗阶段观察到：结果表明，在黑暗阶段，V-EX 主要刺激 5-HTergic 和 DAergic 系统，而 F-EX 则诱发生理应激并增加焦虑样行为。这项研究强调了在行为神经科学实验中考虑运动类型和明暗阶段的重要性。

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Comparison of forced and voluntary exercise types on male rat brain monoamine levels, anxiety-like behaviour, and physiological indexes under light and dark phases

Purpose

Physical exercise improves physical and mental health; however, the differences between voluntary and forced exercise protocols are unclear. In addition, knowledge regarding the consequences of differences in testing timing, such as light and dark phases, in response to exercise type is limited. We investigated the effects of chronic forced and voluntary wheel running on the changes in brain monoamine levels (5-HT: serotonin, DA: dopamine, NA: noradrenaline), anxiety-like behaviours, and physiological stress responses in the light and dark phases.

Methods

Adult male Wistar rats were equally and randomly assigned to four groups: sedentary control, voluntary exercise (free running on a wheel, V-EX), voluntary limited exercise (wheel available only 1 h/day, VL-EX), and forced exercise (running on a motorised wheel, F-EX). Each group was further divided into dark- or light-experimental condition groups. After 4 weeks, the rats underwent an open-field test. The monoamines and their metabolite levels were measured in the major neural cell bodies and the projection areas related to behaviour, cognition, anxiety, and stress in the brain.

Results

Adrenal hypertrophy and elevated body temperature, except during the exercise period, were observed in the F-EX rats that exhibited anxiety-like behaviour. The levels of monoamines and their metabolites, particularly the 5-HTergic and DAergic systems, in specific areas, were significantly altered in the rats in the V-EX group compared to those in the VL-EX and other groups. These differences were observed only in the dark phase.

Conclusion

The results suggest that V-EX mainly stimulates the 5-HTergic and DAergic systems, while F-EX induces physiological stress and increases anxiety-like behaviour during the dark phase. This study highlights the importance of accounting for exercise types and light/dark phases in behavioural neuroscience experiments.

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来源期刊

Behavioural Brain Research 医学-行为科学

CiteScore

5.60

自引率

0.00%

发文量

383

审稿时长

61 days

期刊介绍： Behavioural Brain Research is an international, interdisciplinary journal dedicated to the publication of articles in the field of behavioural neuroscience, broadly defined. Contributions from the entire range of disciplines that comprise the neurosciences, behavioural sciences or cognitive sciences are appropriate, as long as the goal is to delineate the neural mechanisms underlying behaviour. Thus, studies may range from neurophysiological, neuroanatomical, neurochemical or neuropharmacological analysis of brain-behaviour relations, including the use of molecular genetic or behavioural genetic approaches, to studies that involve the use of brain imaging techniques, to neuroethological studies. Reports of original research, of major methodological advances, or of novel conceptual approaches are all encouraged. The journal will also consider critical reviews on selected topics.