开发一种新型环保超声辅助提取法，用于定量检测野生蘑菇中的色氨酸及其有益特性

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-11-12 DOI:10.1016/j.foodchem.2024.142006

Alejandro R. López, Elena Ortega-Caneda, Estrella Espada-Bellido, Nuria Chinchilla, Miguel Palma, María José Aliaño-González, Gerardo Fernández Barbero, Ceferino Carrera

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引用次数: 0

摘要

食物中的生物活性化合物可影响细胞和生理功能，从而为健康带来益处。色氨酸是一种必需氨基酸，也是褪黑激素和血清素等神经递质的前体，可调节情绪和昼夜节律。然而，对蘑菇中色氨酸的定量研究却很少。我们优化了一种生态友好型超声辅助萃取（UAE）方法，利用带二极管阵列和荧光检测器（UHPLC-DAD-Fl）的超高效液相色谱法对色氨酸进行定量。测定了 26 种乳菇属和牛肝菌属野生蘑菇样品中的色氨酸含量。其浓度范围为 0.042 mg/g 至 0.742 mg/g。使用 DPPH（17.7 % - 71.6 %）和 ABTS（7.2 % - 24.9 %）方法评估了提取物的抗氧化能力，而乙酰胆碱酯酶（AChE）抑制活性介于 13.1 % 到 49.8 % 之间。研究结果令人鼓舞。层次聚类分析显示色氨酸浓度、蘑菇种类、地点和提取物特性之间存在相关性，突出了色氨酸在这些蘑菇的保健功效中的关键作用。

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Development of a new eco-friendly ultrasound-assisted extraction method to quantify tryptophan in wild mushrooms and determination of its beneficial properties

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Development of a new eco-friendly ultrasound-assisted extraction method to quantify tryptophan in wild mushrooms and determination of its beneficial properties

Bioactive compounds in food offer health benefits by influencing cellular and physiological functions. Tryptophan, an essential amino acid and precursor to neurotransmitters like melatonin and serotonin, regulates mood and circadian rhythms. However, its quantification in mushrooms is scarce studied. An eco-friendly ultrasound-assisted extraction (UAE) method has been optimized to quantify tryptophan using ultra high-performance liquid chromatography with a diode array and fluorescence detector (UHPLC-DAD-Fl). Tryptophan levels were determined in 26 wild mushroom samples of the genus Lactarius and Boletus. The concentrations ranged from 0.042 mg/g to 0.742 mg/g. The extracts' health benefits were assessed for antioxidant capacity using DPPH (17.7 % - 71.6 %) and ABTS (7.2 % - 24.9 %) methods, while acetylcholinesterase (AChE) inhibitory activity ranged from 13.1 % to 49.8 %. Promising results were obtained. Hierarchical cluster analysis demonstrated a correlation between tryptophan concentration, mushroom species, location, and extract properties, highlighting tryptophan's crucial role in these mushrooms' health benefits.

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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