基于电迁移的三液相膜萃取法辅助高效液相色谱分析牛排样品中的致癌杂环芳香胺

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-11-13 DOI:10.1016/j.foodchem.2024.142037

Fatemeh Barzegar, Samaneh Nabizadeh, Marzieh Kamankesh, Abdorreza Mohammadi, Nabi Shariatifar

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引用次数: 0

摘要

采用微波消解、三相中空纤维膜电萃取（HF-EME）和高效液相色谱（HPLC）相结合的方法测定牛排样品中的致癌杂环胺（HAAs）。所记录的优越性指标（LOD：1.4-1.6 纳克/克，LOQ：4.5-5.2 纳克/克，RSD：6.1-7.2 %，回收率：91-95 %，EF：107-112）强调了所建议技术的适当性。牛排样品中的 2-氨基-3-甲基咪唑并[4,5-f]喹啉（IQ）、2-氨基-3,8-二甲基咪唑并[4,5-f]喹喔啉（MeIQx）、2-氨基-3,4-二甲基咪唑并[4,5-f]喹啉（MeIQ）和 2-氨基-1-甲基-6-苯基咪唑并[4′5-b]吡啶（PhIP）的总含量为 93.46±4.93-174.52±10.91纳克/克、31.65±1.87-877.97±56.19纳克/克、10.08±1.01-535.73±18.93纳克/克和62.56±1.89-341.23±41.45纳克/克。羊排的 MeIQx 含量最高（877.97 ± 56.19 纳克/克），牛肉排的 MeIQx 含量最低（10.08 ± 1.07 纳克/克）。总之，这种新方法具有回收率高、富集因子合适、样品量和有机溶剂需求量极低、萃取时间短等特点，适用于分析复杂食品基质中含量微乎其微的 HAAs。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Three liquid phase membrane extraction method based on the electro-migration assisted by high-performance liquid chromatography for the analysis of carcinogenic heterocyclic aromatic amines in steak samples

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A combination of microwave digestion and three-phase hollow fiber electromembrane extraction (HF-EME) with high-performance liquid chromatography (HPLC) was designed to determine carcinogenic heterocyclic aromatic amines (HAAs) in steak samples. The recorded fingers of merit (LOD: 1.4–1.6 ng/g, LOQ: 4.5–5.2 ng/g, RSD: 6.1–7.2 %, recovery: 91–95 % and EF: 107–112) underlines the adequacy of the proposed technique. The total of 2-amino-3-methylimidazo[4,5-f] quinoline (IQ), 2-amino-3,8-dimethylimidazo [4,5-f] quinoxaline (MeIQx), 2-amino-3,4 dimethylimidazo[4,5-f] quinoline (MeIQ) and 2-amino-1-methyl-6-phenylimidazo[4′5-b] pyridine (PhIP) in steak samples were 93.46 ± 4.93–174.52 ± 10.91 ng/g, 31.65 ± 1.87–877.97 ± 56.19 ng/g, 10.08 ± 1.01–535.73 ± 18.93 ng/g, and 62.56 ± 1.89–341.23 ± 41.45 ng/g, respectively. Lamb steak contain highest amount of MeIQx (877.97 ± 56.19 ng/g) and beef steak showed lowest concentration of MeIQ (10.08 ± 1.07 ng/g). In conclusion, several interesting features such as great recovery, suitable enrichment factor, very low sample amount and organic solvent requirement and short extraction time make this novel method appropriate for the analysis of the negligible levels of HAAs in complex food matrices.

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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