ASpdb：根据实验和人工智能预测结构建立的人类蛋白质同工酶综合知识库

IF 16.6 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nucleic Acids Research Pub Date : 2024-11-12 DOI:10.1093/nar/gkae1018

Yuntao Yang, Himansu Kumar, Yuhan Xie, Zhao Li, Rongbin Li, Wenbo Chen, Chiamaka S Diala, Meer A Ali, Yi Xu, Albon Wu, Sayed-Rzgar Hosseini, Erfei Bi, Hongyu Zhao, Pora Kim, W Jim Zheng

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引用次数: 0

摘要

在真核生物中，替代剪接是一个关键的细胞过程，它能使单个基因产生多种具有不同功能的蛋白质异构体。为了更好地了解替代剪接对蛋白质结构、蛋白质-蛋白质相互作用和人类疾病的影响，我们开发了 ASpdb (https://biodataai.uth.edu/ASpdb/)，这是一个整合了实验测定的结构和 AlphaFold 2 预测的人类蛋白质同工酶模型的综合数据库。ASpdb 包括 3400 多种典型同工酶，每种酶都有实验测定的结构和预测的结构，以及 7200 种带有 AlphaFold 2 预测的替代同工酶。除了详细的剪接事件、三维结构、序列变化和功能注释外，ASpdb 还提供了独特的比较分析和同工酶结构变化的可视化。该资源对于推进替代剪接、结构生物学和疾病机制方面的研究非常有价值。

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ASpdb: an integrative knowledgebase of human protein isoforms from experimental and AI-predicted structures

Alternative splicing is a crucial cellular process in eukaryotes, enabling the generation of multiple protein isoforms with diverse functions from a single gene. To better understand the impact of alternative splicing on protein structures, protein–protein interaction and human diseases, we developed ASpdb (https://biodataai.uth.edu/ASpdb/), a comprehensive database integrating experimentally determined structures and AlphaFold 2-predicted models for human protein isoforms. ASpdb includes over 3400 canonical isoforms, each represented by both experimentally resolved and predicted structures, and >7200 alternative isoforms with AlphaFold 2 predictions. In addition to detailed splicing events, 3D structures, sequence variations and functional annotations, ASpdb uniquely offers comparative analyses and visualization of structural alterations among isoforms. This resource is invaluable for advancing research in alternative splicing, structural biology and disease mechanisms.

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来源期刊

Nucleic Acids Research 生物-生化与分子生物学

CiteScore

27.10

自引率

4.70%

发文量

1057

审稿时长

2 months

期刊介绍： Nucleic Acids Research (NAR) is a scientific journal that publishes research on various aspects of nucleic acids and proteins involved in nucleic acid metabolism and interactions. It covers areas such as chemistry and synthetic biology, computational biology, gene regulation, chromatin and epigenetics, genome integrity, repair and replication, genomics, molecular biology, nucleic acid enzymes, RNA, and structural biology. The journal also includes a Survey and Summary section for brief reviews. Additionally, each year, the first issue is dedicated to biological databases, and an issue in July focuses on web-based software resources for the biological community. Nucleic Acids Research is indexed by several services including Abstracts on Hygiene and Communicable Diseases, Animal Breeding Abstracts, Agricultural Engineering Abstracts, Agbiotech News and Information, BIOSIS Previews, CAB Abstracts, and EMBASE.