用于超分辨率显微镜的纳米银增强型远场荧光波动技术

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2024-11-12 DOI:10.1021/acs.nanolett.4c05155

Guangyong Qin, Xin Guan, Jian Mao, Zhenzhen Feng, Wenxuan Yang, Shenming Wang, Zheng Zhen, Xintong Miao, Yifeng Cheng, Zhirui Wang, Xiaojuan Wang, Fang Huang, Hua He

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引用次数: 0

摘要

基于波动的超分辨显微技术可利用信号波动提高图像分辨率，但荧光团的固有波动限制了其时空分辨率。在这项工作中，我们揭示了一种通过纳米银薄膜产生的远场增强效应（FFE），它能显著增强距离达 10 μm 的荧光团的荧光波动。FFE 效应源于纳米银薄膜的散射光与成像介质中光热引起的折射率变化的干涉，从而在样品上产生周期性的辅助照明。这种现象促成了远场增强超分辨显微镜（FFE-SRM）的发展，这是一种与常用荧光团兼容的技术。与各种 SRM 方法（包括随机光学波动成像、超分辨率径向波动、均值偏移超分辨率和直接随机光学重建显微镜）相比，FFE-SRM 可将时间分辨率提高 10 倍，将空间分辨率提高约 2 倍。我们通过在活细胞成像中揭示线粒体动态、推进超分辨率成像和细胞过程探索，展示了 FFE-SRM 的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Nanosilver-Enhanced Far-Field Fluorescence Fluctuations for Super-Resolution Microscopy

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Nanosilver-Enhanced Far-Field Fluorescence Fluctuations for Super-Resolution Microscopy

Fluctuation-based super-resolution microscopy enhances image resolution using signal fluctuations, yet the inherent fluctuations of fluorophores limit its spatiotemporal resolution. In this work, we reveal a far-field enhancement (FFE) effect via a nanosilver film that significantly boosts fluorescence fluctuations of fluorophores positioned up to 10 μm away. The FFE effect arises from the interference of scattered light from the nanosilver film and photothermal-induced refractive index changes in the imaging medium, which create periodic auxiliary illumination on the sample. This phenomenon enabled the development of far-field enhanced super-resolution microscopy (FFE-SRM), a technique compatible with commonly used fluorophores. FFE-SRM improves temporal resolution up to 10-fold and enhances spatial resolution by about 2-fold over various SRM methods, including stochastic optical fluctuation imaging, super-resolution radial fluctuation, mean-shift super-resolution, and direct stochastic optical reconstruction microscopy. We demonstrated the potential of FFE-SRM by revealing mitochondrial dynamics in live-cell imaging, advancing super-resolution imaging, and cellular process exploration.

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.