{"title":"蝶呤印迹纳米薄膜装饰的 SPR 传感器：从人体血清中灵敏检测蝶呤","authors":"Burcu Eren Yüngeviş, Gözde Baydemir Peşint","doi":"10.1007/s00289-024-05516-2","DOIUrl":null,"url":null,"abstract":"<div><p>Neopterin (Np) is a valuable marker for monitoring infectious diseases and predicting prognosis. This study developed Np-imprinted surface plasmon resonance (SPR) sensors, specifically designed to detect Np in human serum. Characterization of the Np-imprinted (Np-MIP) and non-imprinted (NIP) SPR sensors was carried out using various techniques. The contact angle measurements revealed increased hydrophilicity of the gold sensor with the nanofilm covering, reducing the contact angles from 60.5° to 45.87° and 32.7° for NIP and Np-MIP SPR sensors, respectively. The nanofilm thickness was determined as approximately 14 nm through AFM studies. Real-time Np detection was successfully achieved with the Np-MIP SPR sensors at different concentrations. The selectivity of Np-MIP was confirmed by testing against pterin and glucose, with Np recognition being 11 and 8.25 times higher than those of pterin and glucose, respectively. Np detection from human serum yielded a detection limit of 3 ng/mL. Furthermore, the synthesized Np-MIP SPR sensors demonstrated reusability over 10 cycles without any loss in Np binding capacity. These findings indicate that Np-MIP SPR sensors possess selective detection capabilities for Np in aqueous solutions and human serum.</p></div>","PeriodicalId":737,"journal":{"name":"Polymer Bulletin","volume":"81 18","pages":"17233 - 17252"},"PeriodicalIF":3.1000,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Neopterin imprinted nanofilm decorated SPR sensors: Sensitive neopterin detection from human serum\",\"authors\":\"Burcu Eren Yüngeviş, Gözde Baydemir Peşint\",\"doi\":\"10.1007/s00289-024-05516-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Neopterin (Np) is a valuable marker for monitoring infectious diseases and predicting prognosis. This study developed Np-imprinted surface plasmon resonance (SPR) sensors, specifically designed to detect Np in human serum. Characterization of the Np-imprinted (Np-MIP) and non-imprinted (NIP) SPR sensors was carried out using various techniques. The contact angle measurements revealed increased hydrophilicity of the gold sensor with the nanofilm covering, reducing the contact angles from 60.5° to 45.87° and 32.7° for NIP and Np-MIP SPR sensors, respectively. The nanofilm thickness was determined as approximately 14 nm through AFM studies. Real-time Np detection was successfully achieved with the Np-MIP SPR sensors at different concentrations. The selectivity of Np-MIP was confirmed by testing against pterin and glucose, with Np recognition being 11 and 8.25 times higher than those of pterin and glucose, respectively. Np detection from human serum yielded a detection limit of 3 ng/mL. Furthermore, the synthesized Np-MIP SPR sensors demonstrated reusability over 10 cycles without any loss in Np binding capacity. These findings indicate that Np-MIP SPR sensors possess selective detection capabilities for Np in aqueous solutions and human serum.</p></div>\",\"PeriodicalId\":737,\"journal\":{\"name\":\"Polymer Bulletin\",\"volume\":\"81 18\",\"pages\":\"17233 - 17252\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2024-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polymer Bulletin\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00289-024-05516-2\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"POLYMER SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polymer Bulletin","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00289-024-05516-2","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
Neopterin imprinted nanofilm decorated SPR sensors: Sensitive neopterin detection from human serum
Neopterin (Np) is a valuable marker for monitoring infectious diseases and predicting prognosis. This study developed Np-imprinted surface plasmon resonance (SPR) sensors, specifically designed to detect Np in human serum. Characterization of the Np-imprinted (Np-MIP) and non-imprinted (NIP) SPR sensors was carried out using various techniques. The contact angle measurements revealed increased hydrophilicity of the gold sensor with the nanofilm covering, reducing the contact angles from 60.5° to 45.87° and 32.7° for NIP and Np-MIP SPR sensors, respectively. The nanofilm thickness was determined as approximately 14 nm through AFM studies. Real-time Np detection was successfully achieved with the Np-MIP SPR sensors at different concentrations. The selectivity of Np-MIP was confirmed by testing against pterin and glucose, with Np recognition being 11 and 8.25 times higher than those of pterin and glucose, respectively. Np detection from human serum yielded a detection limit of 3 ng/mL. Furthermore, the synthesized Np-MIP SPR sensors demonstrated reusability over 10 cycles without any loss in Np binding capacity. These findings indicate that Np-MIP SPR sensors possess selective detection capabilities for Np in aqueous solutions and human serum.
期刊介绍：
"Polymer Bulletin" is a comprehensive academic journal on polymer science founded in 1988. It was founded under the initiative of the late Mr. Wang Baoren, a famous Chinese chemist and educator. This journal is co-sponsored by the Chinese Chemical Society, the Institute of Chemistry, and the Chinese Academy of Sciences and is supervised by the China Association for Science and Technology. It is a core journal and is publicly distributed at home and abroad.
"Polymer Bulletin" is a monthly magazine with multiple columns, including a project application guide, outlook, review, research papers, highlight reviews, polymer education and teaching, information sharing, interviews, polymer science popularization, etc. The journal is included in the CSCD Chinese Science Citation Database. It serves as the source journal for Chinese scientific and technological paper statistics and the source journal of Peking University's "Overview of Chinese Core Journals."
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