通过Nrf2/HO-1信号通路改善肉鸡的生长性能和抗氧化能力

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Zhijin Hu, Yibin Qiu, Jiahao Han, Fu Feng, Yunyun Chu, Zhangcheng Li, Liwen Jiang, Zhihong Sun, Lingang Wang, Chen Chen, Zhiru Tang
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引用次数: 0

摘要

本研究的重点是通过Nrf2/HO-1信号通路研究日粮中黄樟叶粉(ZBL)对肉鸡生长性能和抗氧化能力的影响。实验采用单因素完全随机设计,将440只健康的1日龄AA肉鸡随机分为4组:对照组(CON)饲喂以玉米-豆粕为主的日粮;CZ1组饲喂添加1%ZBL粉的基础日粮;CZ2组饲喂添加2%ZBL粉的基础日粮;CZ3组饲喂添加3%ZBL粉的基础日粮。实验分为开食期(1-21 天)和生长期(22-42 天)。初生期每组有五个重复,每个重复有 22 只鸡。生长期的分组与开产期相同,每个重复 20 只鸡。结果表明,在日粮中添加 ZBL 粉对 CZ1 和 CZ2 组肉鸡的生长性能和肠道形态结构没有不良影响(P > 0.05)。与对照组相比,添加 1%和 2%ZBL粉的日粮显著提高了肉鸡血清和肝组织中的 CAT、T-AOC 和 T-SOD 等抗氧化指标、提高了空肠黏膜中Nrf2、NQO1、HO-1、SOD1和CAT等核因子红细胞衍生-2/血红素加氧酶-1(Nrf2/HO-1)信号通路相关基因的表达,增强了空肠黏膜中ZO-1和OCLN基因的表达(P &;lt; 0.05).总之,在日常膳食中分别添加1%和2%的ZBL粉,可通过提高血清和肝脏中的抗氧化剂水平来增强机体的抗氧化能力,上调空肠黏膜中Nrf2/HO-1信号通路相关基因的表达,并有助于维持肠黏膜屏障的完整性。通过二次回归分析确定,日粮中 ZBL 粉的最佳添加量在第 1-21 天为 1.36%-1.60%,在第 22-42 天为 1.14%-1.50%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dietary Zanthoxylum bungeanum leaf powder improves growth performance and antioxidant capacity via Nrf2/HO-1 signaling pathway in broilers
This study focused on the effects of dietary Zanthoxylum bungeanum leaves (ZBL) powder on the growth performance and antioxidant capacity via the Nrf2/HO-1 signaling pathway in broilers. The experiment adopted a single-factor completely random design, and 440 healthy one-day-old AA broiler chicks were randomly divided into four groups: the control group (CON) was fed a corn-soybean meal-based diet, the CZ1 group was fed a basic diet with 1% ZBL powder, the CZ2 group fed a basic diet with 2% ZBL powder, and the CZ3 group fed a basic diet with 3% ZBL powder. The experiment was divided into the starter period (1-21 d) and the grower period (22-42 d). There were five replicates per group in the starter period, with 22 chickens per replicate. The same grouping as in the starter period was used in the grower period, with 20 chickens per replicate. The results showed that the addition of ZBL powder to the diet had no adverse effects on the growth performance and morphological structure of the intestine of broiler chickens in the CZ1 and CZ2 groups (P > 0.05). Compared with the control group, the diet with 1% and 2% ZBL powder significantly increased the antioxidant indicators such as CAT, T-AOC, and T-SOD in the serum and liver tissue of broiler chickens, it upregulated the related genes expression of nuclear factor erythroid derived-2/heme oxygenase-1 (Nrf2/HO-1) signaling pathway such as Nrf2, NQO1, HO-1, SOD1, and CAT in the jejunal mucosa and enhanced the expression of ZO-1 and OCLN genes in the jejunal mucosa (P < 0.05). In conclusion, dietary supplementation with 1% and 2% of ZBL powder into the daily diet can enhance the body's antioxidant capacity by elevating antioxidant levels in both serum and liver, upregulates the expression of genes associated with the Nrf2/HO-1 signaling pathway in the jejunal mucosa, and helps maintain the integrity of the intestinal mucosal barrier. The optimal addition level of ZBL powder in the diet was determined by quadratic regression analysis to be 1.36%-1.60% during days 1-21 and 1.14%-1.50% during days 22-42.
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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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