水稻黄矮病病原菌基因组编码的病毒效应子参与致病过程

IF 6.5 1区生物学 Q1 PLANT SCIENCES

Plant Physiology Pub Date : 2024-11-07 DOI:10.1093/plphys/kiae601

Shuai Zhang, Peng Gan1, Huiting Xie, Chuan Li, Tianxin Tang, Qiong Hu, Zhihong Zhu, Zhongkai Zhang, Jisen Zhang, Yongsheng Zhu, Qun Hu, Jie Hu, Hongxin Guan, Shanshan Zhao, Jianguo Wu

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引用次数: 0

摘要

类细菌植原体在植物和昆虫宿主之间交替繁殖，分泌的蛋白质会破坏宿主的发育。在本研究中，我们利用 PacBio HiFi 技术对与水稻黄矮病（RYD）相关的 "Candidatus Phytoplasma oryzae "菌株 HN2022 进行了全基因组测序。该菌株被归入 16Sr XI-B 亚群。通过 SignalP v5.0 对烟草和水稻（Oryza sativa L.）分泌蛋白的预测和后续表达分析，我们确定了关键的毒力效应蛋白 RY348 和 RY378。RY348 是分泌型翠菊黄化病病原体效应蛋白 54（SAP54）的同源物，能靶向降解 MADS-box 转录因子 MADS1 和 MADS15，导致花粉不育。同时，RY378 会影响绞股蓝内酯和辅助素信号通路，大幅增加分蘖。这些发现为植物与植物病原体之间的相互作用提供了启示。

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Virulence effectors encoded in the rice yellow dwarf phytoplasma genome participate in pathogenesis

Bacteria-like phytoplasmas alternate between plant and insect hosts, secreting proteins that disrupt host development. In this study, we sequenced the complete genome of ‘Candidatus Phytoplasma oryzae’ strain HN2022, associated with rice yellow dwarf (RYD) disease, using PacBio HiFi technology. The strain was classified within the 16Sr XI-B subgroup. Through SignalP v5.0 for prediction and subsequent expression analysis of secreted proteins in Nicotiana benthamiana and rice (Oryza sativa L.), we identified the key virulence effector proteins RY348 and RY378. RY348, a homologue of Secreted Aster Yellows Phytoplasma Effector 54 (SAP54), targets and degrades the MADS-box transcription factors MADS1 and MADS15, causing pollen sterility. Meanwhile, RY378 impacts the strigolactone and auxin signaling pathways, substantially increasing tillering. These findings offer insights into the interactions between plants and phytoplasmas.

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来源期刊

Plant Physiology 生物-植物科学

CiteScore

12.20

自引率

5.40%

发文量

535

审稿时长

2.3 months

期刊介绍： Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.