ARCaDia：拥有潜伏弹头的 DNA 型靶向共价粘合剂的单轮筛选

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2024-11-08 DOI:10.1039/D4CC04594G

Masumi Taki, Masayasu Kuwahara, Chaohui Li, Naoko Tomoda, Naoyuki Miyashita, Tetsuo Kan and Jay Yang

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引用次数: 0

摘要

通过基于亲和力/反应性的合体脱氧核糖核酸（ARCaDia）共选，对潜伏弹头修饰的 DNA 文库进行单轮直接筛选，然后进行顶级 k-mer 分析，从而获得了目标蛋白质的共价结合剂。同时还确定了所选适配体上共轭弹头的最佳位置。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead†

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ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead†

A covalent binder for a target protein was obtained by a direct single-round screening of a latent-warhead-modified DNA library via affinity/reactivity-based co-selection of aptameric deoxyribonucleic acid (ARCaDia), followed by a top k-mer analysis. The optimal position of the conjugated warhead on the selected aptamer was simultaneously identified.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.