基于 SERS 的微流控生物筛选平台用于选择性检测 β 淀粉样肽

IF 3.7 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Langmuir Pub Date : 2024-11-08 DOI:10.1021/acs.langmuir.4c03042

Ankita Jaiswal, Shubham Mishra, Prabhat K. Dwivedi, Sandeep Verma

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引用次数: 0

摘要

本研究报告利用表面增强拉曼光谱（SERS）技术开发了一种微流控装置，用于高灵敏、高选择性地检测模拟脑脊液中的β淀粉样肽（Aβ1-42）。装置组合包括嘌呤配体（Pu）及其与银纳米粒子（AgNPs）的相互作用，以产生 SERS 热点。我们利用合成的嘌呤配体的低表面能和银纳米粒子的高表面能，对嘌呤配体进行功能化并形成了嘌呤-银纳米粒子 SERS 基底。我们采用光刻和软光刻相结合的方法，将新型聚二甲基硅氧烷（PDMS）微流体装置与 Pu-AgNPs 集成在一起，并通过热交联与另一层 PDMS 密封，从而产生了一个有效的 Aβ1-42 筛选平台。来自微流控装置的 SERS 光谱提供了几乎无噪声的测量结果，具有极佳的检测限值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

SERS-Based Microfluidic Bioscreening Platform for Selective Detection of β-Amyloid Peptides

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SERS-Based Microfluidic Bioscreening Platform for Selective Detection of β-Amyloid Peptides

This study reports development of a microfluidic device for highly sensitive and selective detection of a β-amyloid peptide (Aβ_1–42) in simulated cerebrospinal fluid, using surface-enhanced Raman spectroscopy (SERS). The device ensemble comprises a purine ligand (Pu) and its interaction with silver nanoparticles (AgNPs) to generate SERS hotspots. The low surface energy of the synthesized Pu ligand and high surface energy of AgNPs are utilized for the functionalization and formation of a Pu–AgNP SERS substrate. We have integrated a novel polydimethylsiloxane (PDMS) microfluidic device with Pu–AgNPs using a combination of photo- and soft lithography fabrication, sealed by thermal cross-linking with another layer of PDMS, to produce an effective screening platform for Aβ_1–42. The SERS spectrum from the microfluidic device affords almost noise-free measurements, with excellent limit-of-detection values.

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来源期刊

Langmuir 化学-材料科学：综合

CiteScore

6.50

自引率

10.30%

发文量

1464

审稿时长

2.1 months

期刊介绍： Langmuir is an interdisciplinary journal publishing articles in the following subject categories: Colloids: surfactants and self-assembly, dispersions, emulsions, foams Interfaces: adsorption, reactions, films, forces Biological Interfaces: biocolloids, biomolecular and biomimetic materials Materials: nano- and mesostructured materials, polymers, gels, liquid crystals Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do? Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*. This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).