{"title":"PEG600 诱导磷虾油基纳米乳液系统:三相行为和细胞毒性评估","authors":"Anshika Sharma, Arshad Saifi, Anoop Kumar","doi":"10.1186/s43094-024-00720-3","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Endogenous substances of krill oil (KO) are lipophilic in nature and have clinical significance viz. DHA/EPA, phospholipids and astaxanthin. To improve the nanodispersibility of endogenous substances of KO, a self-nanoemulsifying system (SNE) was developed.</p><h3>Results</h3><p>Ternary phase behaviour of KO was explored in ethanol (ET); propylene glycol, (PG); and PEG600 using Tween80 and Tween20 as surfactants. PEG600 induced the self-nanoemulsification of KO and yielded one phase region (OPR); dilution lines (KO/Smix fraction containing PEG600) traversed across OPR, produced a fully dilutable nanoemulsion system. PEG600-based nanoformulations (NFs) of KO underwent phase transformation via percolation behaviour in nanostructure domains (86–207 nm). PEG600 induced ternary phase behaviour of KO as revealed from rheological data (higher eta values), refractive index (nonlinear) and conductivity (bimodal) patterns. Induced phase transformation could be an interaction between aqueous phase and KO/Tween20 in PEG600 environment; generating highly viscous domains of low electrical conductivity. NFs offered antioxidant activity over corresponding coarse systems (<i>p</i> < 0.01) as measured using DPPH method. Optimized NFs (F4 and F6) inhibited the growth of skin cancer cell line (A431) in the range of 100–500 × dilutions.</p><h3>Conclusion</h3><p>Phase behaviour of KO was induced by PEG600, transforming the dilution pattern via generation of one phase region; however, ethanol and propylene glycol as co-solvents did not. PEG600-based NFs of KO possessed antioxidant as well as cytotoxic to skin cancer cell lines (A431).</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00720-3","citationCount":"0","resultStr":"{\"title\":\"PEG600 induced krill oil-based nanoemulsion system: ternary phase behaviour and cytotoxicity assessment\",\"authors\":\"Anshika Sharma, Arshad Saifi, Anoop Kumar\",\"doi\":\"10.1186/s43094-024-00720-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Endogenous substances of krill oil (KO) are lipophilic in nature and have clinical significance viz. DHA/EPA, phospholipids and astaxanthin. To improve the nanodispersibility of endogenous substances of KO, a self-nanoemulsifying system (SNE) was developed.</p><h3>Results</h3><p>Ternary phase behaviour of KO was explored in ethanol (ET); propylene glycol, (PG); and PEG600 using Tween80 and Tween20 as surfactants. PEG600 induced the self-nanoemulsification of KO and yielded one phase region (OPR); dilution lines (KO/Smix fraction containing PEG600) traversed across OPR, produced a fully dilutable nanoemulsion system. PEG600-based nanoformulations (NFs) of KO underwent phase transformation via percolation behaviour in nanostructure domains (86–207 nm). PEG600 induced ternary phase behaviour of KO as revealed from rheological data (higher eta values), refractive index (nonlinear) and conductivity (bimodal) patterns. Induced phase transformation could be an interaction between aqueous phase and KO/Tween20 in PEG600 environment; generating highly viscous domains of low electrical conductivity. NFs offered antioxidant activity over corresponding coarse systems (<i>p</i> < 0.01) as measured using DPPH method. Optimized NFs (F4 and F6) inhibited the growth of skin cancer cell line (A431) in the range of 100–500 × dilutions.</p><h3>Conclusion</h3><p>Phase behaviour of KO was induced by PEG600, transforming the dilution pattern via generation of one phase region; however, ethanol and propylene glycol as co-solvents did not. 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引用次数: 0
摘要
背景磷虾油(KO)的内源性物质具有亲脂性,并具有临床意义,如 DHA/EPA、磷脂和虾青素。为了提高 KO 内源物质的纳米分散性,我们开发了一种自纳米乳化系统 (SNE)。结果以 Tween80 和 Tween20 为表面活性剂,研究了 KO 在乙醇 (ET)、丙二醇 (PG) 和 PEG600 中的三相行为。PEG600 诱导了 KO 的自纳米乳化,并产生了一个相区(OPR);稀释线(KO/含 PEG600 的混合馏分)穿过 OPR,产生了一个完全可稀释的纳米乳液体系。以 PEG600 为基础的 KO 纳米制剂(NFs)在纳米结构域(86-207 纳米)中通过渗流行为发生相变。流变学数据(更高的等值)、折射率(非线性)和电导率(双峰)模式显示,PEG600 诱导了 KO 的三元相行为。诱导相变可能是水相与 PEG600 环境中的 KO/Tween20 之间的相互作用;产生了低导电率的高粘度域。用 DPPH 法测量,NFs 的抗氧化活性高于相应的粗糙体系(p < 0.01)。结论 PEG600 可诱导 KO 的相行为,通过生成一个相区来改变稀释模式;而作为助溶剂的乙醇和丙二醇则不会。基于 PEG600 的 KO NFs 具有抗氧化性,并对皮肤癌细胞株 (A431) 具有细胞毒性。
Endogenous substances of krill oil (KO) are lipophilic in nature and have clinical significance viz. DHA/EPA, phospholipids and astaxanthin. To improve the nanodispersibility of endogenous substances of KO, a self-nanoemulsifying system (SNE) was developed.
Results
Ternary phase behaviour of KO was explored in ethanol (ET); propylene glycol, (PG); and PEG600 using Tween80 and Tween20 as surfactants. PEG600 induced the self-nanoemulsification of KO and yielded one phase region (OPR); dilution lines (KO/Smix fraction containing PEG600) traversed across OPR, produced a fully dilutable nanoemulsion system. PEG600-based nanoformulations (NFs) of KO underwent phase transformation via percolation behaviour in nanostructure domains (86–207 nm). PEG600 induced ternary phase behaviour of KO as revealed from rheological data (higher eta values), refractive index (nonlinear) and conductivity (bimodal) patterns. Induced phase transformation could be an interaction between aqueous phase and KO/Tween20 in PEG600 environment; generating highly viscous domains of low electrical conductivity. NFs offered antioxidant activity over corresponding coarse systems (p < 0.01) as measured using DPPH method. Optimized NFs (F4 and F6) inhibited the growth of skin cancer cell line (A431) in the range of 100–500 × dilutions.
Conclusion
Phase behaviour of KO was induced by PEG600, transforming the dilution pattern via generation of one phase region; however, ethanol and propylene glycol as co-solvents did not. PEG600-based NFs of KO possessed antioxidant as well as cytotoxic to skin cancer cell lines (A431).
期刊介绍:
Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.