基于吸附在碳纳米管上的 PQQ 依赖性醇脱氢酶直接电子传递的高灵敏通量型无创酒精生物传感器†。

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Citra Dewi Rakhmania, Yoshi Izzuddin Azhar, Kenji Shida, Erika Shinchi, Taiki Adachi, Keisei Sowa, Yuki Kitazumi, Osamu Shirai and Masato Tominaga
{"title":"基于吸附在碳纳米管上的 PQQ 依赖性醇脱氢酶直接电子传递的高灵敏通量型无创酒精生物传感器†。","authors":"Citra Dewi Rakhmania, Yoshi Izzuddin Azhar, Kenji Shida, Erika Shinchi, Taiki Adachi, Keisei Sowa, Yuki Kitazumi, Osamu Shirai and Masato Tominaga","doi":"10.1039/D4SD00161C","DOIUrl":null,"url":null,"abstract":"<p >Ethanol gas excreted by human skin can be used to determine auto-brewery syndrome (drunken disease), blood alcohol levels, and/or a body state of alcoholism. Considering the limitations of continuous non-invasive alcohol gas monitoring based on the electrochemical method, which requires high sensitivity and selectivity, a CNF film sensor was developed. This sensor was developed by utilizing pyrroloquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) and multiwalled carbon nanotubes (MWCNTs) based on cellulose nanofiber (CNF) film platform. With a compact design, a PQQ-ADH/MWCNTs/CNF film sensor was built in a three-electrode system. This system could continuously detect ethanol gas with ultra-high sensitivity, a wide detection range (24 ppb–25 ppm), and high selectivity for ethanol. Finally, the CNF film sensor was used for ethanol gas monitoring in the human subject, and we were able to detect metabolism abnormalities of the subject by analyzing the declining slope (detoxification rate) of the ethanol gas concentration monitored. The CNF film sensor aims to gain valuable insights and enhance future standard health screening practices through non-invasive wearable daily monitoring sensors.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00161c?page=search","citationCount":"0","resultStr":"{\"title\":\"Highly sensitive flux-type non-invasive alcohol biosensor based on direct electron transfer of PQQ-dependent alcohol dehydrogenases adsorbed on carbon nanotubes†\",\"authors\":\"Citra Dewi Rakhmania, Yoshi Izzuddin Azhar, Kenji Shida, Erika Shinchi, Taiki Adachi, Keisei Sowa, Yuki Kitazumi, Osamu Shirai and Masato Tominaga\",\"doi\":\"10.1039/D4SD00161C\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Ethanol gas excreted by human skin can be used to determine auto-brewery syndrome (drunken disease), blood alcohol levels, and/or a body state of alcoholism. Considering the limitations of continuous non-invasive alcohol gas monitoring based on the electrochemical method, which requires high sensitivity and selectivity, a CNF film sensor was developed. This sensor was developed by utilizing pyrroloquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) and multiwalled carbon nanotubes (MWCNTs) based on cellulose nanofiber (CNF) film platform. With a compact design, a PQQ-ADH/MWCNTs/CNF film sensor was built in a three-electrode system. This system could continuously detect ethanol gas with ultra-high sensitivity, a wide detection range (24 ppb–25 ppm), and high selectivity for ethanol. Finally, the CNF film sensor was used for ethanol gas monitoring in the human subject, and we were able to detect metabolism abnormalities of the subject by analyzing the declining slope (detoxification rate) of the ethanol gas concentration monitored. The CNF film sensor aims to gain valuable insights and enhance future standard health screening practices through non-invasive wearable daily monitoring sensors.</p>\",\"PeriodicalId\":74786,\"journal\":{\"name\":\"Sensors & diagnostics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00161c?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sensors & diagnostics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/sd/d4sd00161c\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors & diagnostics","FirstCategoryId":"1085","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/sd/d4sd00161c","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

摘要

人体皮肤排出的乙醇气体可用于确定自酿综合征(醉酒病)、血液酒精含量和/或酗酒的身体状态。基于电化学方法的连续无创酒精气体监测需要高灵敏度和高选择性,考虑到这种方法的局限性,我们开发了一种 CNF 薄膜传感器。该传感器是在纤维素纳米纤维(CNF)薄膜平台上利用吡咯喹啉醌依赖性酒精脱氢酶(PQQ-ADH)和多壁碳纳米管(MWCNTs)开发的。PQQ-ADH/MWCNTs/CNF 薄膜传感器设计紧凑,采用三电极系统。该系统可连续检测乙醇气体,具有超高灵敏度、宽检测范围(24 ppb-25 ppm)和对乙醇的高选择性。最后,我们将 CNF 薄膜传感器用于人体乙醇气体监测,并通过分析监测到的乙醇气体浓度的下降斜率(解毒率)来检测人体的代谢异常。CNF 薄膜传感器旨在通过非侵入式可穿戴日常监测传感器获得有价值的见解,并加强未来的标准健康检查实践。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Highly sensitive flux-type non-invasive alcohol biosensor based on direct electron transfer of PQQ-dependent alcohol dehydrogenases adsorbed on carbon nanotubes†

Highly sensitive flux-type non-invasive alcohol biosensor based on direct electron transfer of PQQ-dependent alcohol dehydrogenases adsorbed on carbon nanotubes†

Ethanol gas excreted by human skin can be used to determine auto-brewery syndrome (drunken disease), blood alcohol levels, and/or a body state of alcoholism. Considering the limitations of continuous non-invasive alcohol gas monitoring based on the electrochemical method, which requires high sensitivity and selectivity, a CNF film sensor was developed. This sensor was developed by utilizing pyrroloquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) and multiwalled carbon nanotubes (MWCNTs) based on cellulose nanofiber (CNF) film platform. With a compact design, a PQQ-ADH/MWCNTs/CNF film sensor was built in a three-electrode system. This system could continuously detect ethanol gas with ultra-high sensitivity, a wide detection range (24 ppb–25 ppm), and high selectivity for ethanol. Finally, the CNF film sensor was used for ethanol gas monitoring in the human subject, and we were able to detect metabolism abnormalities of the subject by analyzing the declining slope (detoxification rate) of the ethanol gas concentration monitored. The CNF film sensor aims to gain valuable insights and enhance future standard health screening practices through non-invasive wearable daily monitoring sensors.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.30
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信