从八种地衣中提取的多酚的细胞毒性潜力及其对癌细胞株的抗氧化活性

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Łukasz Furmanek , Natalia Żurek , Ireneusz Kapusta , Mark R.D. Seaward , Paweł Czarnota
{"title":"从八种地衣中提取的多酚的细胞毒性潜力及其对癌细胞株的抗氧化活性","authors":"Łukasz Furmanek ,&nbsp;Natalia Żurek ,&nbsp;Ireneusz Kapusta ,&nbsp;Mark R.D. Seaward ,&nbsp;Paweł Czarnota","doi":"10.1016/j.bcab.2024.103424","DOIUrl":null,"url":null,"abstract":"<div><div>This article presents the results of <em>in vitro</em> experiments on the cytotoxic potential of ethanol extracts from 8 lichen species, <em>Cetraria islandica</em>, <em>Cladonia arbuscula</em>, <em>C. digitata</em>, <em>C. gracilis</em>, <em>C. rangiferina</em>, <em>C. uncialis</em>, <em>Platismatia glauca</em> and <em>Pseudevernia furfuracea</em>, against 5 human cancer cell lines - MCF-7, Caco-2, SK-mel-28, U87MG and Jurkat.</div><div>The potential of the re-dissolved and then tested extracts was determined by means of TPC, free radical scavenging activity in ABTS<sup>•+</sup>, superoxide and hydroxyl tests, copper ion reduction activity, and chelating ability of ferrous ion. The highest TPC (114.29 mg GAE/g), ABTS<sup>•+</sup> (440.61 mmol TE/100 g) and hydroxyl (IC<sub>50</sub>: 0.71 mg/mL) radical scavenging activities tests was obtained for the <em>Pseudevernia furfuracea</em> extract, and for the superoxide radical scavenging activity test (IC<sub>50</sub>: 0.98 mg/mL), the highest potential was found for the extracts from <em>P. furfuracea</em> and <em>Cladonia digitata</em>, and for the copper ion reduction activity (88.15 mmol TE/100 g) and chelating ability of ferrous ion (IC<sub>50</sub>: 1.83 mg/mL) tests, the highest potential was shown for the extract from <em>C. digitata</em>. In the cytotoxicity tests, the strongest potential was shown by the <em>P. furfuracea</em> extract against the MCF-7 (IC<sub>50</sub>: 110.84 μg/mL), Caco-2 (IC<sub>50</sub>: 123.86 μg/mL) and U87MG (IC<sub>50</sub>: 107.43 μg/mL) cancer cell lines. A wound scratch test against the MCF-7 using <em>Pseudevernia furfuracea</em> extract showed a 50% reduction in tumor cell proliferation. The qualitative - 28 secondary metabolites from depside and depsidones classes - and quantitative profile of lichen extracts was analyzed by UPLC-Q-TOF-MS/MS.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The cytotoxic potential of polyphenols extracted from eight lichen species and their antioxidant activity against the cancer cell lines\",\"authors\":\"Łukasz Furmanek ,&nbsp;Natalia Żurek ,&nbsp;Ireneusz Kapusta ,&nbsp;Mark R.D. Seaward ,&nbsp;Paweł Czarnota\",\"doi\":\"10.1016/j.bcab.2024.103424\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This article presents the results of <em>in vitro</em> experiments on the cytotoxic potential of ethanol extracts from 8 lichen species, <em>Cetraria islandica</em>, <em>Cladonia arbuscula</em>, <em>C. digitata</em>, <em>C. gracilis</em>, <em>C. rangiferina</em>, <em>C. uncialis</em>, <em>Platismatia glauca</em> and <em>Pseudevernia furfuracea</em>, against 5 human cancer cell lines - MCF-7, Caco-2, SK-mel-28, U87MG and Jurkat.</div><div>The potential of the re-dissolved and then tested extracts was determined by means of TPC, free radical scavenging activity in ABTS<sup>•+</sup>, superoxide and hydroxyl tests, copper ion reduction activity, and chelating ability of ferrous ion. The highest TPC (114.29 mg GAE/g), ABTS<sup>•+</sup> (440.61 mmol TE/100 g) and hydroxyl (IC<sub>50</sub>: 0.71 mg/mL) radical scavenging activities tests was obtained for the <em>Pseudevernia furfuracea</em> extract, and for the superoxide radical scavenging activity test (IC<sub>50</sub>: 0.98 mg/mL), the highest potential was found for the extracts from <em>P. furfuracea</em> and <em>Cladonia digitata</em>, and for the copper ion reduction activity (88.15 mmol TE/100 g) and chelating ability of ferrous ion (IC<sub>50</sub>: 1.83 mg/mL) tests, the highest potential was shown for the extract from <em>C. digitata</em>. In the cytotoxicity tests, the strongest potential was shown by the <em>P. furfuracea</em> extract against the MCF-7 (IC<sub>50</sub>: 110.84 μg/mL), Caco-2 (IC<sub>50</sub>: 123.86 μg/mL) and U87MG (IC<sub>50</sub>: 107.43 μg/mL) cancer cell lines. A wound scratch test against the MCF-7 using <em>Pseudevernia furfuracea</em> extract showed a 50% reduction in tumor cell proliferation. The qualitative - 28 secondary metabolites from depside and depsidones classes - and quantitative profile of lichen extracts was analyzed by UPLC-Q-TOF-MS/MS.</div></div>\",\"PeriodicalId\":8774,\"journal\":{\"name\":\"Biocatalysis and agricultural biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-10-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biocatalysis and agricultural biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1878818124004080\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocatalysis and agricultural biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1878818124004080","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

本文介绍了从 8 种地衣(Cetraria islandica、Cladonia arbuscula、C. digitata、C. gracilis、C. rangiferina、C. uncialis、Platismatia glauca 和 Pseudevernia furfuracea)中提取的乙醇提取物对 5 种人类癌细胞系(MCF-7、Caco-2、SK-mel-28、U87MG 和 Jurkat)的细胞毒性潜力的体外实验结果。通过 TPC、ABTS-+、超氧化物和羟基测试中的自由基清除活性、铜离子还原活性和亚铁离子螯合能力,测定了重新溶解后进行测试的提取物的潜力。在 TPC(114.29 毫克 GAE/克)、ABTS-+(440.61 毫摩尔 TE/100 克)和羟基(IC50:0.71 毫克/毫升)自由基清除活性测试中,糠假毛蕊花提取物的最高;在超氧自由基清除活性测试中(IC50:0.在铜离子还原活性(88.15 mmol TE/100 g)和亚铁离子螯合能力(IC50:1.83 mg/mL)测试中,C. digitata 提取物的潜力最大。在细胞毒性测试中,糠虾提取物对 MCF-7(IC50:110.84 μg/mL)、Caco-2(IC50:123.86 μg/mL)和 U87MG(IC50:107.43 μg/mL)癌细胞株的毒性最强。使用假 fevernia furfuracea 提取物对 MCF-7 进行的伤口划痕试验显示,肿瘤细胞的增殖减少了 50%。通过 UPLC-Q-TOF-MS/MS 分析了地衣提取物的定性(28 种去苷类和去苷酮类次生代谢物)和定量特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The cytotoxic potential of polyphenols extracted from eight lichen species and their antioxidant activity against the cancer cell lines

The cytotoxic potential of polyphenols extracted from eight lichen species and their antioxidant activity against the cancer cell lines
This article presents the results of in vitro experiments on the cytotoxic potential of ethanol extracts from 8 lichen species, Cetraria islandica, Cladonia arbuscula, C. digitata, C. gracilis, C. rangiferina, C. uncialis, Platismatia glauca and Pseudevernia furfuracea, against 5 human cancer cell lines - MCF-7, Caco-2, SK-mel-28, U87MG and Jurkat.
The potential of the re-dissolved and then tested extracts was determined by means of TPC, free radical scavenging activity in ABTS•+, superoxide and hydroxyl tests, copper ion reduction activity, and chelating ability of ferrous ion. The highest TPC (114.29 mg GAE/g), ABTS•+ (440.61 mmol TE/100 g) and hydroxyl (IC50: 0.71 mg/mL) radical scavenging activities tests was obtained for the Pseudevernia furfuracea extract, and for the superoxide radical scavenging activity test (IC50: 0.98 mg/mL), the highest potential was found for the extracts from P. furfuracea and Cladonia digitata, and for the copper ion reduction activity (88.15 mmol TE/100 g) and chelating ability of ferrous ion (IC50: 1.83 mg/mL) tests, the highest potential was shown for the extract from C. digitata. In the cytotoxicity tests, the strongest potential was shown by the P. furfuracea extract against the MCF-7 (IC50: 110.84 μg/mL), Caco-2 (IC50: 123.86 μg/mL) and U87MG (IC50: 107.43 μg/mL) cancer cell lines. A wound scratch test against the MCF-7 using Pseudevernia furfuracea extract showed a 50% reduction in tumor cell proliferation. The qualitative - 28 secondary metabolites from depside and depsidones classes - and quantitative profile of lichen extracts was analyzed by UPLC-Q-TOF-MS/MS.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Biocatalysis and agricultural biotechnology
Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
7.70
自引率
2.50%
发文量
308
审稿时长
48 days
期刊介绍: Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信