Łukasz Furmanek , Natalia Żurek , Ireneusz Kapusta , Mark R.D. Seaward , Paweł Czarnota
{"title":"从八种地衣中提取的多酚的细胞毒性潜力及其对癌细胞株的抗氧化活性","authors":"Łukasz Furmanek , Natalia Żurek , Ireneusz Kapusta , Mark R.D. Seaward , Paweł Czarnota","doi":"10.1016/j.bcab.2024.103424","DOIUrl":null,"url":null,"abstract":"<div><div>This article presents the results of <em>in vitro</em> experiments on the cytotoxic potential of ethanol extracts from 8 lichen species, <em>Cetraria islandica</em>, <em>Cladonia arbuscula</em>, <em>C. digitata</em>, <em>C. gracilis</em>, <em>C. rangiferina</em>, <em>C. uncialis</em>, <em>Platismatia glauca</em> and <em>Pseudevernia furfuracea</em>, against 5 human cancer cell lines - MCF-7, Caco-2, SK-mel-28, U87MG and Jurkat.</div><div>The potential of the re-dissolved and then tested extracts was determined by means of TPC, free radical scavenging activity in ABTS<sup>•+</sup>, superoxide and hydroxyl tests, copper ion reduction activity, and chelating ability of ferrous ion. The highest TPC (114.29 mg GAE/g), ABTS<sup>•+</sup> (440.61 mmol TE/100 g) and hydroxyl (IC<sub>50</sub>: 0.71 mg/mL) radical scavenging activities tests was obtained for the <em>Pseudevernia furfuracea</em> extract, and for the superoxide radical scavenging activity test (IC<sub>50</sub>: 0.98 mg/mL), the highest potential was found for the extracts from <em>P. furfuracea</em> and <em>Cladonia digitata</em>, and for the copper ion reduction activity (88.15 mmol TE/100 g) and chelating ability of ferrous ion (IC<sub>50</sub>: 1.83 mg/mL) tests, the highest potential was shown for the extract from <em>C. digitata</em>. In the cytotoxicity tests, the strongest potential was shown by the <em>P. furfuracea</em> extract against the MCF-7 (IC<sub>50</sub>: 110.84 μg/mL), Caco-2 (IC<sub>50</sub>: 123.86 μg/mL) and U87MG (IC<sub>50</sub>: 107.43 μg/mL) cancer cell lines. A wound scratch test against the MCF-7 using <em>Pseudevernia furfuracea</em> extract showed a 50% reduction in tumor cell proliferation. The qualitative - 28 secondary metabolites from depside and depsidones classes - and quantitative profile of lichen extracts was analyzed by UPLC-Q-TOF-MS/MS.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103424"},"PeriodicalIF":3.4000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The cytotoxic potential of polyphenols extracted from eight lichen species and their antioxidant activity against the cancer cell lines\",\"authors\":\"Łukasz Furmanek , Natalia Żurek , Ireneusz Kapusta , Mark R.D. Seaward , Paweł Czarnota\",\"doi\":\"10.1016/j.bcab.2024.103424\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This article presents the results of <em>in vitro</em> experiments on the cytotoxic potential of ethanol extracts from 8 lichen species, <em>Cetraria islandica</em>, <em>Cladonia arbuscula</em>, <em>C. digitata</em>, <em>C. gracilis</em>, <em>C. rangiferina</em>, <em>C. uncialis</em>, <em>Platismatia glauca</em> and <em>Pseudevernia furfuracea</em>, against 5 human cancer cell lines - MCF-7, Caco-2, SK-mel-28, U87MG and Jurkat.</div><div>The potential of the re-dissolved and then tested extracts was determined by means of TPC, free radical scavenging activity in ABTS<sup>•+</sup>, superoxide and hydroxyl tests, copper ion reduction activity, and chelating ability of ferrous ion. The highest TPC (114.29 mg GAE/g), ABTS<sup>•+</sup> (440.61 mmol TE/100 g) and hydroxyl (IC<sub>50</sub>: 0.71 mg/mL) radical scavenging activities tests was obtained for the <em>Pseudevernia furfuracea</em> extract, and for the superoxide radical scavenging activity test (IC<sub>50</sub>: 0.98 mg/mL), the highest potential was found for the extracts from <em>P. furfuracea</em> and <em>Cladonia digitata</em>, and for the copper ion reduction activity (88.15 mmol TE/100 g) and chelating ability of ferrous ion (IC<sub>50</sub>: 1.83 mg/mL) tests, the highest potential was shown for the extract from <em>C. digitata</em>. In the cytotoxicity tests, the strongest potential was shown by the <em>P. furfuracea</em> extract against the MCF-7 (IC<sub>50</sub>: 110.84 μg/mL), Caco-2 (IC<sub>50</sub>: 123.86 μg/mL) and U87MG (IC<sub>50</sub>: 107.43 μg/mL) cancer cell lines. A wound scratch test against the MCF-7 using <em>Pseudevernia furfuracea</em> extract showed a 50% reduction in tumor cell proliferation. 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The cytotoxic potential of polyphenols extracted from eight lichen species and their antioxidant activity against the cancer cell lines
This article presents the results of in vitro experiments on the cytotoxic potential of ethanol extracts from 8 lichen species, Cetraria islandica, Cladonia arbuscula, C. digitata, C. gracilis, C. rangiferina, C. uncialis, Platismatia glauca and Pseudevernia furfuracea, against 5 human cancer cell lines - MCF-7, Caco-2, SK-mel-28, U87MG and Jurkat.
The potential of the re-dissolved and then tested extracts was determined by means of TPC, free radical scavenging activity in ABTS•+, superoxide and hydroxyl tests, copper ion reduction activity, and chelating ability of ferrous ion. The highest TPC (114.29 mg GAE/g), ABTS•+ (440.61 mmol TE/100 g) and hydroxyl (IC50: 0.71 mg/mL) radical scavenging activities tests was obtained for the Pseudevernia furfuracea extract, and for the superoxide radical scavenging activity test (IC50: 0.98 mg/mL), the highest potential was found for the extracts from P. furfuracea and Cladonia digitata, and for the copper ion reduction activity (88.15 mmol TE/100 g) and chelating ability of ferrous ion (IC50: 1.83 mg/mL) tests, the highest potential was shown for the extract from C. digitata. In the cytotoxicity tests, the strongest potential was shown by the P. furfuracea extract against the MCF-7 (IC50: 110.84 μg/mL), Caco-2 (IC50: 123.86 μg/mL) and U87MG (IC50: 107.43 μg/mL) cancer cell lines. A wound scratch test against the MCF-7 using Pseudevernia furfuracea extract showed a 50% reduction in tumor cell proliferation. The qualitative - 28 secondary metabolites from depside and depsidones classes - and quantitative profile of lichen extracts was analyzed by UPLC-Q-TOF-MS/MS.
期刊介绍:
Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.