KRT35 和 TCHHL1 功能基因与辽宁绒山羊羊绒细度的相关性和回归分析

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology

Journal of Genetic Engineering and Biotechnology Pub Date : 2024-11-06 DOI:10.1016/j.jgeb.2024.100434

Weihang Hong , Hua Ma , Lingjun Nie , Shuaitong Li , Lingchao Kong , Ran Duan , Qingyu Yuan , Qiying Zhan , Jinghan Wang , Yuyan Cong , Zeying Wang

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LCG cashmere has moderate fineness (the average fineness of cashmere of LCG population is about 16 µm).However, as a slightly coarse textile raw material, we hope to identify the key genes regulating cashmere fineness through PCR-seq and MLR, in order to reduce cashmere fineness.We collected and extracted DNA from the blood of Liaoning cashmere goats, designed primers, PCR amplification, and Statistical analysis. It was found that the the AA genotype of the G3667A locus of the <em>KRT35</em> gene, CT genotype of the T615C locus of the <em>TCHHL1</em> gene in bucks and the CC genotype of does, as well as CT genotype of the T615C locus of the <em>TCHHL1</em> gene in bucks and the CC genotype of does are dominant genotypes in cashmere fineness. The dominant haplotype combination with multiple factors and effects of cashmere fineness has been determined to be CTGG in bucks and TTGG in does. There was a significant linear regression relationship between the fineness of cashmere in LCG and the cashmere rate and cashmere quantity. There is a significant linear regression relationship between the fineness of LCG and the cashmere rate and cashmere quantity. CF = 0.001SQ-0.71CY + 20.784 (R<sup>2</sup> = 0.818) in buck and CF = 0.001SQ-0.767CY + 22.009 (R<sup>2</sup> = 0.863) in doe. 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引用次数: 0

摘要

辽宁绒山羊（LCG）是世界上产绒量最高的白绒山羊。它具有绒纤维长、净绒率高、绒细度适中、绒毛洁白、体型健壮、适应性强、遗传性能稳定、改良中低产绒山羊效果好等特点。被誉为 "中国国宝"。以 LCG 为父本，培育出了 5 个地方新品种，为中国绒山羊品种改良和育种做出了突出贡献。LCG山羊绒的细度适中（LCG种群山羊绒的平均细度约为16微米），但作为一种略粗的纺织原料，我们希望通过PCR-seq和MLR找出调控山羊绒细度的关键基因，以降低山羊绒的细度。结果发现，KRT35基因 G3667A位点的AA基因型、TCHHL1基因T615C位点的CT基因型（公山羊）和CC基因型（母山羊）以及TCHHL1基因T615C位点的CT基因型（公山羊）和CC基因型（母山羊）是羊绒细度的显性基因型。在多种因素和羊绒细度影响下，公鹿的显性单倍型组合被确定为 CTGG，母鹿的显性单倍型组合被确定为 TTGG。LCG 羊绒细度与羊绒率和羊绒量之间存在明显的线性回归关系。LCG 的细度与羊绒率和羊绒量之间存在明显的线性回归关系。公鹿的 CF = 0.001SQ-0.71CY + 20.784 (R2 = 0.818)，母鹿的 CF = 0.001SQ-0.767CY + 22.009 (R2 = 0.863)。结论KRT35 基因的 AA 基因型、公鹿 TCHHL1 基因的 CT 基因型和母鹿的 CC 基因型可用作分子标记，帮助选择羊绒细度。

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Correlation and regression analysis of KRT35 and TCHHL1 functional genes for cashmere fineness in Liaoning cashmere goats

Liaoning cashmere goat (LCG) is the world’s highest cashmere producing white cashmere goat. It has the characteristics of long cashmere fiber, high net cashmere rate, moderate cashmere fineness, white cashmere, strong size, strong adaptability, stable genetic performance, and good effect in improving middle and low production cashmere goat. It is known as “National treasure of China”. With LCG as the paternal parent, five new local breeds have been cultivated, which has made outstanding contributions to the improvement and breeding of Chinese cashmere goat breeds. LCG cashmere has moderate fineness (the average fineness of cashmere of LCG population is about 16 µm).However, as a slightly coarse textile raw material, we hope to identify the key genes regulating cashmere fineness through PCR-seq and MLR, in order to reduce cashmere fineness.We collected and extracted DNA from the blood of Liaoning cashmere goats, designed primers, PCR amplification, and Statistical analysis. It was found that the the AA genotype of the G3667A locus of the KRT35 gene, CT genotype of the T615C locus of the TCHHL1 gene in bucks and the CC genotype of does, as well as CT genotype of the T615C locus of the TCHHL1 gene in bucks and the CC genotype of does are dominant genotypes in cashmere fineness. The dominant haplotype combination with multiple factors and effects of cashmere fineness has been determined to be CTGG in bucks and TTGG in does. There was a significant linear regression relationship between the fineness of cashmere in LCG and the cashmere rate and cashmere quantity. There is a significant linear regression relationship between the fineness of LCG and the cashmere rate and cashmere quantity. CF = 0.001SQ-0.71CY + 20.784 (R² = 0.818) in buck and CF = 0.001SQ-0.767CY + 22.009 (R² = 0.863) in doe. Conclusion: The AA genotype of KRT35 gene, CT genotype of TCHHL1 gene in bucks and CC genotype of does can be used as molecular markers to assist in the selection of cashmere fineness.

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来源期刊

Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

5.70

自引率

5.70%

发文量

159

审稿时长

16 weeks

期刊介绍： Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts