利用糖尿病小鼠模型观察中性粒细胞胞外陷阱在糖尿病肾病发展过程中的作用。

IF 2.7 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
You Hyun Jeon, Se-Hyun Oh, Soo-Jung Jung, Eun-Joo Oh, Jeong-Hoon Lim, Hee-Yeon Jung, Ji-Young Choi, Sun-Hee Park, Chan-Duck Kim, Yong-Lim Kim, Chang-Won Hong, Jang-Hee Cho
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引用次数: 0

摘要

背景:糖尿病肾病(DN)是糖尿病患者的一种进展性并发症,也是导致终末期肾病的最常见原因。众所周知,中性粒细胞胞外捕获物(NET)在肾脏疾病中发挥作用,因此本研究旨在利用糖尿病小鼠模型确定它们在糖尿病肾脏疾病发展中的作用:蛋白质和组织学分析表明,db/db小鼠和链脲佐菌素DN模型没有表达明显的NET相关蛋白、髓过氧化物酶、瓜氨酸组蛋白H3(citH3)、中性粒细胞弹性蛋白酶和淋巴细胞抗原6复合位点G6D(Ly6G)。然而,根据免疫组化图像,DN 模型中的炎症个体显示 citH3 和 Ly6G 在肾脏系统中高度沉积。在体外,NET 处理不会诱导肾小球内皮细胞和肾小管上皮细胞凋亡。通过使用 DNase 抑制 NET,细胞凋亡没有明显变化:结论:NET 相关蛋白仅在伴有肾小管间质炎症的 DN 模型中表达。我们的研究表明,NET 只在高血糖诱导炎症的小鼠中被诱导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Observation of neutrophil extracellular traps in the development of diabetic nephropathy using diabetic murine models.

Background: Diabetic nephropathy (DN) is a progressive complication among patients with diabetes and the most common cause of end-stage kidney disease. Neutrophil extracellular traps (NETs) are known to play a role in kidney disease, thus this study aimed to determine their role in the development of diabetic kidney disease using diabetic murine models.

Results: Protein and histological analyses revealed that db/db mice and streptozotocin DN models expressed no significant NET-related proteins, myeloperoxidase, citrullinated histone H3 (citH3), neutrophil elastase, and lymphocyte antigen 6 complex locus G6D (Ly6G). However, the inflamed individuals in the DN model showed that citH3 and Ly6G were highly deposited in the renal system based on immunohistochemistry images. In vitro, NET treatment did not induce apoptosis in glomerular endothelial and renal tubular epithelial cells. NET inhibition by DNase administration demonstrated no significant changes in cell apoptosis.

Conclusions: NET-related proteins were only expressed in the DN model with tubulointerstitial inflammation. Our study revealed that NETs are only induced in mice with hyperglycemia-induced inflammation.

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来源期刊
CiteScore
4.40
自引率
0.00%
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32
审稿时长
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