豆科植物中非根瘤菌和丛枝菌根真菌的分离与鉴定。

IF 4 2区 生物学 Q2 MICROBIOLOGY
Mohamed Hemida Abd-Alla, Nivien A Nafady, Amany A Hassan, Shymaa R Bashandy
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引用次数: 0

摘要

本研究调查了鹰嘴豆(Cicer arietinum L)、蚕豆(Vicia faba)和豇豆(Vigna unguiculata L. Walp)根瘤中的非根瘤菌,以及鹰嘴豆和蚕豆根瘤土壤中的丛枝菌根真菌。在检测的 34 个内生细菌种群中,有 31 个菌株根据结核试验被确定为非根瘤菌。对所有菌株进行了体外植物生长促进(PGP)活性评估。结果显示,大多数分离菌株表现出多种 PGP 活性,如固氮、产生吲哚-3-乙酸(IAA)和氨(NH3)、溶解磷酸盐和产生外多糖。筛选出最有效的 PGP 细菌进行 16S rRNA 分析。此外,共鉴定出 36 种本地丛枝菌根真菌(AMF)。Acaulospora (100%)和 Scutellospora (91.66%)分别是 Cicer arietinum L. 和 Vicia faba L. 植物中最普遍的菌属。Acaulospora 在这两种植物中的孢子密度和相对丰度也最高。此外,Cicer arietinum L.和 Vicia faba L.植株根部的菌丝、囊泡和假根(HVA)定殖也很显著。这项研究的结果为了解与豆科植物根瘤相关的非根瘤菌内生细菌和 AMF 的多样性提供了宝贵的信息。这些生物在 PGP 方面具有巨大潜力,可以通过与根瘤菌共同接种来提高其生物肥料效果。这种操作对促进可持续农业、改善作物生长和推进生物肥料技术至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and characterization of non-rhizobial bacteria and arbuscular mycorrhizal fungi from legumes.

This study investigates non-rhizobial endophytic bacteria in the root nodules of chickpea (Cicer arietinum L), faba bean (Vicia faba), and cowpea (Vigna unguiculata L. Walp), as well as arbuscular mycorrhizal fungi in the rhizospheric soil of chickpea and faba bean. Out of the 34 endophytic bacterial populations examined, 31 strains were identified as non-rhizobial based on nodulation tests. All strains were assessed for their plant growth-promoting (PGP) activities in vitro. The results revealed that most isolates exhibited multiple PGP activities, such as nitrogen fixation, indole-3-acetic acid (IAA) and ammonia (NH3) production, phosphate solubilization, and exopolysaccharide production. The most effective PGP bacteria were selected for 16S rRNA analysis. Additionally, a total of 36 species of native arbuscular mycorrhizal fungi (AMF) were identified. Acaulospora (100%) and Scutellospora (91.66%) were the most prevalent genera in Cicer arietinum L. and Vicia faba L. plants, respectively. Acaulospora also exhibited the highest spore density and relative abundance in both plants. Moreover, the root colonization of Cicer arietinum L. and Vicia faba L. plants by hyphae, vesicles, and arbuscules (HVA) was significant. The findings of this study provide valuable insights into non-rhizobial endophytic bacteria associated with legume root nodules and the diversity of AMF. These organisms have great potential for PGP and can be manipulated by co-inoculation with rhizobia to enhance their biofertilizer effectiveness. This manipulation is crucial for promoting sustainable agriculture, improving crop growth, and advancing biofertilizer technology.

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来源期刊
BMC Microbiology
BMC Microbiology 生物-微生物学
CiteScore
7.20
自引率
0.00%
发文量
280
审稿时长
3 months
期刊介绍: BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.
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