使用 2bRAD-M 对单侧结石形成者的男女肾盂尿生物群进行比较。

IF 4 2区 生物学 Q2 MICROBIOLOGY
Sen-Yuan Hong, Lin-Tao Miao, Yuan-Yuan Yang, Shao-Gang Wang
{"title":"使用 2bRAD-M 对单侧结石形成者的男女肾盂尿生物群进行比较。","authors":"Sen-Yuan Hong, Lin-Tao Miao, Yuan-Yuan Yang, Shao-Gang Wang","doi":"10.1186/s12866-024-03618-5","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Urolithiasis is a prevalent urological ailment characterized by increasing prevalence and recurrence rates, resulting in substantial social and economic burden. While men exhibit an incidence rate nearly twice that of women, this gender disparity is gradually diminishing. Nevertheless, the mechanisms underlying this condition remain incompletely elucidated. The identification of the urinary microbiome (urobiome) has provided a fresh perspective on urolithiasis. This study aimed to analyze the urobiome of unilateral stone formers in the renal pelvis and evaluate the variations in microbial diversity and community composition between males and females.</p><p><strong>Methods: </strong>Renal pelvis urine samples were obtained from a cohort of 21 male and 9 female patients and subsequently subjected to taxonomic and functional analysis using 2bRAD sequencing for Microbiome (2bRAD-M). The collected samples were categorized into four distinct groups, namely the stone side of males (SM), stone side of females (SF), non-stone side of males (NSM), and non-stone side of females (NSF).</p><p><strong>Results: </strong>Through the application of beta diversity analysis, dissimilarity was observed between NSM and NSF. Additionally, NSF exhibited a higher abundance of microbial populations, and a total of 29 distinct species were identified as differentially present between NSM and NSF using LEfSe. Lactobacillus iners, Atopobium deltae, Lawsonella clevelandensis, and Meyerozyma guilliermondii exhibited enrichment in the SF group compared to the SM group. Furthermore, we identified distinct species that differed between the SM and NSM groups, as well as the SF and NSF groups. Besides, we conducted COG annotation and KEGG pathway predictions, revealing significant differences in urobiome function across the different groups.</p><p><strong>Conclusion: </strong>Variations in microbial community composition and predicted functions were observed among the various groups. Future research could potentially leverage the urobiome to personalize urolithiasis treatment based on individual microbial characteristics, taking into account gender-specific differences.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"24 1","pages":"456"},"PeriodicalIF":4.0000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11539328/pdf/","citationCount":"0","resultStr":"{\"title\":\"A comparison of male and female renal pelvis urobiome of unilateral stone formers using 2bRAD-M.\",\"authors\":\"Sen-Yuan Hong, Lin-Tao Miao, Yuan-Yuan Yang, Shao-Gang Wang\",\"doi\":\"10.1186/s12866-024-03618-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Urolithiasis is a prevalent urological ailment characterized by increasing prevalence and recurrence rates, resulting in substantial social and economic burden. While men exhibit an incidence rate nearly twice that of women, this gender disparity is gradually diminishing. Nevertheless, the mechanisms underlying this condition remain incompletely elucidated. The identification of the urinary microbiome (urobiome) has provided a fresh perspective on urolithiasis. This study aimed to analyze the urobiome of unilateral stone formers in the renal pelvis and evaluate the variations in microbial diversity and community composition between males and females.</p><p><strong>Methods: </strong>Renal pelvis urine samples were obtained from a cohort of 21 male and 9 female patients and subsequently subjected to taxonomic and functional analysis using 2bRAD sequencing for Microbiome (2bRAD-M). The collected samples were categorized into four distinct groups, namely the stone side of males (SM), stone side of females (SF), non-stone side of males (NSM), and non-stone side of females (NSF).</p><p><strong>Results: </strong>Through the application of beta diversity analysis, dissimilarity was observed between NSM and NSF. Additionally, NSF exhibited a higher abundance of microbial populations, and a total of 29 distinct species were identified as differentially present between NSM and NSF using LEfSe. Lactobacillus iners, Atopobium deltae, Lawsonella clevelandensis, and Meyerozyma guilliermondii exhibited enrichment in the SF group compared to the SM group. Furthermore, we identified distinct species that differed between the SM and NSM groups, as well as the SF and NSF groups. Besides, we conducted COG annotation and KEGG pathway predictions, revealing significant differences in urobiome function across the different groups.</p><p><strong>Conclusion: </strong>Variations in microbial community composition and predicted functions were observed among the various groups. Future research could potentially leverage the urobiome to personalize urolithiasis treatment based on individual microbial characteristics, taking into account gender-specific differences.</p>\",\"PeriodicalId\":9233,\"journal\":{\"name\":\"BMC Microbiology\",\"volume\":\"24 1\",\"pages\":\"456\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-11-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11539328/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1186/s12866-024-03618-5\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s12866-024-03618-5","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:泌尿系结石是一种常见的泌尿系统疾病,其发病率和复发率不断上升,造成了巨大的社会和经济负担。虽然男性的发病率几乎是女性的两倍,但这种性别差异正在逐渐缩小。然而,这种疾病的发病机制仍未完全阐明。泌尿微生物组(urobiome)的鉴定为尿路结石病提供了一个全新的视角。本研究旨在分析单侧肾盂结石患者的尿液微生物组,并评估男性和女性之间微生物多样性和群落组成的差异:从21名男性患者和9名女性患者的肾盂尿液样本中获取样本,然后使用2bRAD微生物组测序技术(2bRAD-M)对样本进行分类和功能分析。采集的样本被分为四组,即男性结石侧(SM)、女性结石侧(SF)、男性非结石侧(NSM)和女性非结石侧(NSF):结果:通过贝塔多样性分析,观察到 NSM 和 NSF 之间存在差异。此外,NSF 表现出更高的微生物种群丰度,使用 LEfSe 共鉴定出 29 个不同物种在 NSM 和 NSF 之间存在差异。与 SM 组相比,SF 组中的 Iners 乳杆菌、Atopobium deltae、Lawsonella clevelandensis 和 Meyerozyma guilliermondii 表现出富集。此外,我们还发现了SM组与NSM组之间以及SF组与NSF组之间的不同物种。此外,我们还进行了COG注释和KEGG通路预测,发现不同组的尿生物群功能存在显著差异:结论:不同组别的微生物群落组成和预测功能存在差异。未来的研究有可能利用尿生物群,根据个体微生物的特征,并考虑性别差异,对尿路结石进行个性化治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A comparison of male and female renal pelvis urobiome of unilateral stone formers using 2bRAD-M.

Background: Urolithiasis is a prevalent urological ailment characterized by increasing prevalence and recurrence rates, resulting in substantial social and economic burden. While men exhibit an incidence rate nearly twice that of women, this gender disparity is gradually diminishing. Nevertheless, the mechanisms underlying this condition remain incompletely elucidated. The identification of the urinary microbiome (urobiome) has provided a fresh perspective on urolithiasis. This study aimed to analyze the urobiome of unilateral stone formers in the renal pelvis and evaluate the variations in microbial diversity and community composition between males and females.

Methods: Renal pelvis urine samples were obtained from a cohort of 21 male and 9 female patients and subsequently subjected to taxonomic and functional analysis using 2bRAD sequencing for Microbiome (2bRAD-M). The collected samples were categorized into four distinct groups, namely the stone side of males (SM), stone side of females (SF), non-stone side of males (NSM), and non-stone side of females (NSF).

Results: Through the application of beta diversity analysis, dissimilarity was observed between NSM and NSF. Additionally, NSF exhibited a higher abundance of microbial populations, and a total of 29 distinct species were identified as differentially present between NSM and NSF using LEfSe. Lactobacillus iners, Atopobium deltae, Lawsonella clevelandensis, and Meyerozyma guilliermondii exhibited enrichment in the SF group compared to the SM group. Furthermore, we identified distinct species that differed between the SM and NSM groups, as well as the SF and NSF groups. Besides, we conducted COG annotation and KEGG pathway predictions, revealing significant differences in urobiome function across the different groups.

Conclusion: Variations in microbial community composition and predicted functions were observed among the various groups. Future research could potentially leverage the urobiome to personalize urolithiasis treatment based on individual microbial characteristics, taking into account gender-specific differences.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
BMC Microbiology
BMC Microbiology 生物-微生物学
CiteScore
7.20
自引率
0.00%
发文量
280
审稿时长
3 months
期刊介绍: BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信